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| Registros recuperados: 65 | |
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| PASSAMANI,PAULO Z.; CARVALHO,CARLOS R.; SOARES,FERNANDA A.F.. |
| ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cultures were used to obtain metaphases from male and female individuals. The chromosomes were amplified via PRINS, and subcentromeric fragments of the X chromosome were microdissected using microneedles coupled to a phase contrast microscope. The fragments were amplified by DOP-PCR and labeled with tetramethyl-rhodamine-5-dUTP. The probes were used in fluorescent in situ hybridization (FISH) procedure to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DOP-PCR; FISH; Micromanipulation; PRINS. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100041 |
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| Vieira-Pinto,M.; Oliveira,M.; Bernardo,F.; Martins,C.. |
| This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P<0.001). From positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Salmonella; FISH; ELFA. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600006 |
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| Fradkin,Maia; Greizerstein,Eduardo J; Ferrari,María R; Poggio,Lidia. |
| "Trigopiros" derive from crosses between different species of Triticum L. and Thinopyrum Löve. These synthetic amphiploids are designed with the aim to obtain cereals similar to wheat but which are perennial, resistant to diseases and to the salinity of the soils. Moreover, they allow the transfer of the agronomic attributes of Thinopyrum to wheat. "Trigopiro" Don Noé INTA, which is currently grown in Argentina, presents valuable agronomic traits as well as a high content of seed proteins. In the present work, the use of classical cytogenetic techniques allowed us to confirm that the chromosome number of "Trigopiro" Don Noé is 2n=56. The application of in situ hybridization (FISH-GISH) allowed us to postulate its genomic composition for the first time.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trigopiro; FISH; GISH. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722014000400011 |
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| Lorscheider,Carla Andreia; Oliveira,Jordana Inácio Nascimento; Dulz,Thais Aparecida; Nogaroto,Viviane; Martins-Santos,Isabel Cristina; Vicari,Marcelo Ricardo. |
| ABSTRACT The karyotypes of three armored catfish species (Loricariidae) from the Iguaçu river, southern of the Brazil, were compared using different techniques: C-banding, Ag-NOR and fluorescence in situ hybridization (FISH), which used 5S and 18S rDNAs and total Cot-1 fraction as probes. Hypostomus commersoni and Hypostomus derbyi presented 2n = 68 chromosomes, with karyotype formulae 12m+12sm+14st+30a and 12m+12sm+10st+34a, respectively; whereas Hypostomus myersi presented 2n = 74 chromosomes and 12m+16sm+12st+34a. The chromosomal localization of the Ag-NORs, 5S and 18S rDNAs differed in number of sites and chromosomal localization among the studied species. The total Cot-1 probe permitted the visualization of the repetitive DNA fraction in karyotypes of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Armored catfish; FISH; Iguaçu river; Karyotype evolution. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100435 |
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| Pinheiro,R.F.; Chauffaille,M.L.L.F.. |
| Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Myelodysplastic syndrome; FISH; G-banding. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100018 |
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| Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J.. |
| Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006 |
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| Tabatabaei,Meisam; Zakaria,Mohd Rafein; Rahim,Raha Abdul; Wright,André-Denis G.; Shirai,Yoshihito; Abdullah,Norhani; Sakai,Kenji; Ikeno,Shinya; Mori,Masatsugu; Kazunori,Nakamura; Sulaiman,Alawi; Hassan,Mohd Ali. |
| 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens,... |
| Tipo: Journal article |
Palavras-chave: Anaerobic digestion; DGGE; FISH; Methanogens; POME. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012 |
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| Nirchio,Mauro; Oliveira,Claudio; Ferreira,Irani Alves; Granado,Angel; Ron,Ernesto. |
| The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 18S rDNA; Ag-NORs; C-band; FISH; Fish cytogenetics; Sex chromosomes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100007 |
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| Veiga,Luciana CS; Bérgamo,Nádia A; Kowalski,Luiz Paulo; Rogatto,Silvia R. |
| Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: FISH; Chromosomal aberrations; Head and neck cancer; Chromosome 17. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003 |
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| Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo. |
| Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010 |
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| Anjos,Allison; Loreto,Vilma; Souza,Maria José de. |
| In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006 |
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| Registros recuperados: 65 | |
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