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| Registros recuperados: 27 | |
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| Zepeta-Flores,Nahum; Valverde,Mahara; Lopez-Saavedra,Alejandro; Rojas,Emilio. |
| Abstract The importance of glutathione (GSH) in alternative cellular roles to the canonically proposed, were analyzed in a model unable to synthesize GSH. Gene expression analysis shows that the regulation of the actin cytoskeleton pathway is strongly impacted by the absence of GSH. To test this hypothesis, we evaluate the effect of GSH depletion via buthionine sulfoximine (5 and 12.5 mM) in human neuroblastoma MSN cells. In the present study, 70% of GSH reduction did not induce reactive oxygen species, lipoperoxidation, or cytotoxicity, which enabled us to evaluate the effect of glutathione in the absence of oxidative stress. The cells with decreasing GSH levels acquired morphology changes that depended on the actin cytoskeleton and not on tubulin. We... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; BSO; Thymosin β4; Gelsolin; Profiling. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300475 |
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| Sitja Bobadilla, A; Pena Llopis, S; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J. |
| Partial or total replacement of fish meal by a mixture of plant protein (PP) sources (corn gluten, wheat gluten, extruded peas, rapeseed meal and sweet white lupin) balanced with indispensable amino acids was examined in juvenile gilthead sea bream over the course of a 6-month growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, and PP100). The possible influence of diets on growth performance, plasma metabolites, gut integrity, liver structure, anti-oxidant and immune status was evaluated. Final body weight was progressively decreased with PP inclusion, but in PP50 and PP75-fed fish, feed efficiency (FE) was significantly improved and specific growth rates remained... |
| Tipo: Text |
Palavras-chave: Gilthead sea bream; Cholesterol; Glutathione; Histopathology; Myeloperoxidase; Complement; Lysozyme; Respiratory burst; Immune response; Growth; Plant proteins; Fish meal. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3595.pdf |
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| Gru, Cecile; Sarradin, Pierre-marie; Legoff, Hélène; Narcon, Sandrine; Caprais, Jean-claude; Lallier, Francois. |
| This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to... |
| Tipo: Text |
Palavras-chave: Sulfide; Sulfite; Thiosulfate; Cysteine; Glutathione; Hydrothermal seawater; Riftia pachyptila; Biological fluids. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00186/29763/28203.pdf |
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| El-Shahat,Abd El-Rahman; Gabr,Attia; Meki,Abdel-Raheim; Mehana,El-Saed. |
| Cadmium (Cd), is an environmental and industrial pollutant that affects the male reproductive system. Cd induces its effect by affecting tissue antioxidant enzyme systems. Green tea extract (GTE) is an antioxidant and free radicals scavenger and has a chelating property. The purpose of this study was to investigate the protective effect of GTE against testes damage induced by Cd. Four groups of male rats, were utilized as following: Controls, GTE treated, Cd treated and Cd + GTE, treated rats at the same doses. The rats received GTE and or Cd orally in drinking water. After 5 weeks, the animals were sacrificed and testes were removed for microscopic and Biochemical evaluation. The levels of lipid peroxides (LPO) and glutathione (GSH) were detected in the... |
| Tipo: Journal article |
Palavras-chave: Rat Testes; Cadmium toxicity; Green tea extract; Lipid peroxides; Glutathione. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300020 |
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| Andrioli,N.B; Chaufan,G; Coalova,I; Ríos de Molina,M.C; Mudry,M.D. |
| Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 μM, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 μM concentrations by using the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Genotoxicity; Glutathione; HEp-2 cell line. |
| Ano: 2017 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000300002 |
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| Hassan,AMA; Mostafa,EM. |
| Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages were 78% and 60%, respectively at 1 ppm Se in comparison with the control. At higher Se concentrations (>5 ppm),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Selenium; Azolla caroliniana; Ascorbate; Glutathione; Antioxidant enzymes; Glutathione peroxidase. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200014 |
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| MOHAMED,N.; TAHA,R.M.; RAZAK,U.N.A.A.. |
| ABSTRACT: In this study, the micropropagation of Impatiens balsamina was established from stem and shoot explants. The effects of GA3 and glutathione on the morphogenesis of this species were also investigated, in order to induce in vitro flowering. It was found that the optimum in vitro plant regeneration was achieved on MS medium supplemented with 1.0 mg L-1 GA3 and in vitro flowering was also obtained from the same medium after 4 weeks of culture. To understand cellular behavior during in vitro flowering, Mitotic Index (MI), chromosome counts, measurement of mean cell and nuclear areas, DNA measurements and ploidy levels were analyzed from in vivo plants, in vitro grown plants and plantlets that flowered in vitro. The chromosome count was the same for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Regeneration; Gibberellic acid; Glutathione; Flowering response; Cellular behavior and acclimatization. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100236 |
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| Wang,D; Wang,D; Pu,L; Wei,G. |
| ABSTRACT The aim of this study was to assess the effect of selenium-enriched Candida utilis with high contents of organic selenium (Se) and glutathione (GSH) on growth performance, antioxidant capacity and immune function of broiler chickens. A total of 100 healthy 7-day-old male broiler chickens were randomly divided into 5 groups, and fed diets supplemented with (a) Na2SeO3, (b) C. utilis, (c) Se-enriched Saccharomyces cerevisiae, (d) Se-enriched C. utilis, and (e) the control without any supplements. The experiment lasted for 6 weeks and parameters were recorded on day 42. No significant differences in average daily gain were found among the 5 groups. However, Se-enriched C. utilis supplemented in the diet increased activities of glutathione peroxidase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant capacity; Broiler chickens; Glutathione; Immune function; Selenium-enriched Candida utilis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200314 |
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| Silva,Adinan Alves da; Oliveira,Juraci Alves de; Campos,Fernanda Vidal de; Ribeiro,Cleberson; Farnese,Fernanda dos Santos. |
| ABSTRACT In many plant species, tolerance to toxic metals is highly dependent on glutathione, an essential metabolite for cellular detoxification. We evaluated the responses of glutathione metabolism to arsenite (AsIII) in Salvinia molesta, an aquatic fern that has unexplored phytoremediation potential. Plants were exposed to different AsIII concentrations in nutrient solution for 24 h. AsIII caused cell membrane damage to submerged leaves, indicating oxidative stress. There was an increase in the glutathione content and ϒ-glutamylcysteine synthetase enzyme activity in the submerged and floating leaves. The glutathione peroxidase and glutathione sulfotransferase enzymes also showed increased activity in both plant parts, whereas glutathione reductase only... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arsenic; Glutathione; Macrophytes; Oxidative stress; Phytoremediation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400657 |
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| Stojiljković,Vesnać; Pejić,SneAna; Kasapović,Jelena; Gavrilović,Ljubicać; Stojiljković,Stanimirć; Nikolić,Draganć; Pajović,SneAna B.. |
| The celiac disease is an autoimmune gastrointestinal disorder caused by gluten from wheat, rye or barley. In genetically predisposed persons, gluten induces the immune-mediated inflammation of small intestinal mucosa. Histological lesions include intraepithelial lymphocytosis, crypt hypertrophy and villous atrophy, resulting in malabsorption of micro- and macronutrients. The only treatment for celiac patients is a permanent gluten-free diet (GFD). Reactive oxygen species (ROS) and oxidative stress are strongly associated with the celiac disease. Glutathione (GSH) is a main detoxifier of endogenous and exogenous ROS in the intestine. In order to explain the role of glutathione redox cycle in celiac patients, we examined the activities of GSH-related... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant enzymes; Celiac disease; Glutathione. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100018 |
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| Martins,V.E.D.; Pinto,S.C.C.; Chaves,R.M.; Barros Filho,A.K.D.; Laskoski,L.M.; Souza,F.A.. |
| ABSTRACT This study aimed to evaluate the addition of Vitamin C, reduced Glutathione and trolox on sperm characteristics of pork refrigerated semen. Six pigs were collected through the technique of gloved hand (10 ejaculates/animals). The semen was diluted in MR-A®. After the previous evaluations, the treatments were added: Control group: diluent only; Vitamin C Group: 200μM/mL Vitamin C; Trolox Group: 200μM/mL Trolox; Glutathione group: 2.5mM/ml Reduced glutathione. The semen was stored in thermal boxes and placed inside the refrigerator at 15oC and evaluated at D0, 12, 48, 72 hours. After 30 hours of incubation, each treatment was divided into two equal fractions and the same concentration of antioxidants was added in one of the parts. The results show... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; Sperm; Trolox; Vitamin C. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100145 |
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| MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO. |
| Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,... |
| Tipo: Journal article |
Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007 |
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| Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte. |
| The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016 |
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| Treitinger,Aricio; Spada,Celso; Masokawa,Ivete Yoshico; Verdi,Júlio César Vidal; Silveira,Mariette Van Der Sander; Luis,Magali Chaves; Reis,Marcellus; Ferreira,Silvia Inês Alejandra Cordova de Pires; Abdalla,Dulcinéia Saes Parra. |
| N-acetyl-L-cysteine (NAC) has been proposed as an additional therapeutic agent for AIDS patients because it reduces human immunodeficiency virus type 1 (HIV-1) replication in stimulated CD4+ lymphocytes, and it ameliorates immunological reactivity. In a randomized, 180-day, double-blind, placebo-controlled trial performed with HIV-infected patients classified as A2 and A3 according to the criteria of the Center for Disease Control and Prevention, we investigated the effects of oral administration of NAC on HIV-infected patients undergoing their first anti-retroviral therapy; viral load, CD4+ lymphocyte, lymphocyte viability and apoptosis, and TNF-alpha and IL-8 levels were determined. Sixteen patients who received anti-retroviral therapy plus a placebo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: N-acetylcysteine; Apoptosis; Viability; HIV; Glutathione; TNF-alpha; IL-8. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500005 |
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| Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S.. |
| The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004 |
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| De Angelis,K.L.D.; Cestari,I.A.; Barp,J.; Dall'Ago,P.; Fernandes,T.G.; Homem de Bittencourt,P.I.; Belló-Klein,A.; Belló,A.A.; Llesuy,S.; Irigoyen,M.C.. |
| The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Streptozotocin-diabetes; Latissimus dorsi muscle; Oxidative stress; Catalase; Glutathione. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100016 |
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| Registros recuperados: 27 | |
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