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| Registros recuperados: 29 | |
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| Silva,Adinan Alves da; Oliveira,Juraci Alves de; Campos,Fernanda Vidal de; Ribeiro,Cleberson; Farnese,Fernanda dos Santos. |
| ABSTRACT In many plant species, tolerance to toxic metals is highly dependent on glutathione, an essential metabolite for cellular detoxification. We evaluated the responses of glutathione metabolism to arsenite (AsIII) in Salvinia molesta, an aquatic fern that has unexplored phytoremediation potential. Plants were exposed to different AsIII concentrations in nutrient solution for 24 h. AsIII caused cell membrane damage to submerged leaves, indicating oxidative stress. There was an increase in the glutathione content and ϒ-glutamylcysteine synthetase enzyme activity in the submerged and floating leaves. The glutathione peroxidase and glutathione sulfotransferase enzymes also showed increased activity in both plant parts, whereas glutathione reductase only... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arsenic; Glutathione; Macrophytes; Oxidative stress; Phytoremediation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400657 |
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| Stojiljković,Vesnać; Pejić,SneAna; Kasapović,Jelena; Gavrilović,Ljubicać; Stojiljković,Stanimirć; Nikolić,Draganć; Pajović,SneAna B.. |
| The celiac disease is an autoimmune gastrointestinal disorder caused by gluten from wheat, rye or barley. In genetically predisposed persons, gluten induces the immune-mediated inflammation of small intestinal mucosa. Histological lesions include intraepithelial lymphocytosis, crypt hypertrophy and villous atrophy, resulting in malabsorption of micro- and macronutrients. The only treatment for celiac patients is a permanent gluten-free diet (GFD). Reactive oxygen species (ROS) and oxidative stress are strongly associated with the celiac disease. Glutathione (GSH) is a main detoxifier of endogenous and exogenous ROS in the intestine. In order to explain the role of glutathione redox cycle in celiac patients, we examined the activities of GSH-related... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant enzymes; Celiac disease; Glutathione. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100018 |
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| Martins,V.E.D.; Pinto,S.C.C.; Chaves,R.M.; Barros Filho,A.K.D.; Laskoski,L.M.; Souza,F.A.. |
| ABSTRACT This study aimed to evaluate the addition of Vitamin C, reduced Glutathione and trolox on sperm characteristics of pork refrigerated semen. Six pigs were collected through the technique of gloved hand (10 ejaculates/animals). The semen was diluted in MR-A®. After the previous evaluations, the treatments were added: Control group: diluent only; Vitamin C Group: 200μM/mL Vitamin C; Trolox Group: 200μM/mL Trolox; Glutathione group: 2.5mM/ml Reduced glutathione. The semen was stored in thermal boxes and placed inside the refrigerator at 15oC and evaluated at D0, 12, 48, 72 hours. After 30 hours of incubation, each treatment was divided into two equal fractions and the same concentration of antioxidants was added in one of the parts. The results show... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; Sperm; Trolox; Vitamin C. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100145 |
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| MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO. |
| Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,... |
| Tipo: Journal article |
Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007 |
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| Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte. |
| The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016 |
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| Treitinger,Aricio; Spada,Celso; Masokawa,Ivete Yoshico; Verdi,Júlio César Vidal; Silveira,Mariette Van Der Sander; Luis,Magali Chaves; Reis,Marcellus; Ferreira,Silvia Inês Alejandra Cordova de Pires; Abdalla,Dulcinéia Saes Parra. |
| N-acetyl-L-cysteine (NAC) has been proposed as an additional therapeutic agent for AIDS patients because it reduces human immunodeficiency virus type 1 (HIV-1) replication in stimulated CD4+ lymphocytes, and it ameliorates immunological reactivity. In a randomized, 180-day, double-blind, placebo-controlled trial performed with HIV-infected patients classified as A2 and A3 according to the criteria of the Center for Disease Control and Prevention, we investigated the effects of oral administration of NAC on HIV-infected patients undergoing their first anti-retroviral therapy; viral load, CD4+ lymphocyte, lymphocyte viability and apoptosis, and TNF-alpha and IL-8 levels were determined. Sixteen patients who received anti-retroviral therapy plus a placebo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: N-acetylcysteine; Apoptosis; Viability; HIV; Glutathione; TNF-alpha; IL-8. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500005 |
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| De Angelis,K.L.D.; Cestari,I.A.; Barp,J.; Dall'Ago,P.; Fernandes,T.G.; Homem de Bittencourt,P.I.; Belló-Klein,A.; Belló,A.A.; Llesuy,S.; Irigoyen,M.C.. |
| The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Streptozotocin-diabetes; Latissimus dorsi muscle; Oxidative stress; Catalase; Glutathione. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100016 |
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| Coimbra,C.G.; Junqueira,V.B.C.. |
| Abnormal riboflavin status in the absence of a dietary deficiency was detected in 31 consecutive outpatients with Parkinson's disease (PD), while the classical determinants of homocysteine levels (B6, folic acid, and B12) were usually within normal limits. In contrast, only 3 of 10 consecutive outpatients with dementia without previous stroke had abnormal riboflavin status. The data for 12 patients who did not complete 6 months of therapy or did not comply with the proposed treatment paradigm were excluded from analysis. Nineteen PD patients (8 males and 11 females, mean age ± SD = 66.2 ± 8.6 years; 3, 3, 2, 5, and 6 patients in Hoehn and Yahr stages I to V) received riboflavin orally (30 mg every 8 h) plus their usual symptomatic medications and all red... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parkinson's disease; Riboflavin; Flavin-adenine dinucleotide; Glutathione; Iron; Hemin. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000019 |
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| Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S.. |
| The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004 |
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| Ferraz,H.B.; Quagliato,E.A.B.; Rieder,C.R.M.; Silva,D.J.; Teive,H.A.G.; Barbosa,E.R.; Cardoso,F.; Limongi,J.C.P.; Bezerra,J.M.F.; Andrade,L.A.F.; Allam,N.; Prado,R.C.P.; Tomas,V.. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Parkinson's disease; Riboflavin; FAD; Glutathione; Iron; Hemin. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900002 |
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| Romão,P.R.T.; Tovar,J.; Fonseca,S.G.; Moraes,R.H.; Cruz,A.K.; Hothersall,J.S.; Noronha-Dutra,A.A.; Ferreira,S.H.; Cunha,F.Q.. |
| Glutathione is the major intracellular antioxidant thiol protecting mammalian cells against oxidative stress induced by oxygen- and nitrogen-derived reactive species. In trypanosomes and leishmanias, trypanothione plays a central role in parasite protection against mammalian host defence systems by recycling trypanothione disulphide by the enzyme trypanothione reductase. Although Kinetoplastida parasites lack glutathione reductase, they maintain significant levels of glutathione. The aim of this study was to use Leishmania donovani trypanothione reductase gene mutant clones and different Leishmania species to examine the role of these two individual thiol systems in the protection mechanism against S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Glutathione; Trypanothione; Trypanothione reductase; Nitric oxide; Free radicals. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300006 |
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| Manfredini,V.; Roehrs,R.; Peralba,M.C.R.; Henriques,J.A.P.; Saffi,J.; Ramos,A.L.L.P.; Benfato,M.S.. |
| Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Hydrogen peroxide; Saccharomyces cerevisiae; Reactive oxygen species. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200001 |
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| Öztürk,L.K.; Furuncuoğlu,H.; Atala,M.H.; Uluköylü,O.; Akyüz,S.; Yarat,A.. |
| The aim of the present study was to evaluate the relationship between salivary oxidative stress and dental-oral health. Healthy young adults, matched for gender and age, with (N = 21, 10 men, mean age: 20.3 ± 1 years) and without (N = 16, 8 men, mean age: 21.2 ± 1.8 years) caries were included in this study. The World Health Organization (WHO) caries diagnostic criteria were used for determining the decayed, missing, filled teeth (DMFT) index. The oral hygiene and gingival status were assessed using the simplified oral hygiene index and gingival index, respectively. Unstimulated salivary total protein, glutathione (GSH), lipid peroxidation and total sialic acid levels, carbonic anhydrase activity, and salivary buffering capacity were determined by standard... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Glutathione; Lipid peroxidation; Sialic acid; Carbonic anhydrase; Dental-oral health. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100003 |
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| Ozen,Tevfik; Yildirim,Kemal; Toka,Mehmet. |
| ABSTRACT In this work, the potential chemopreventive activities of Elaeagnus umbellata fruit aqueous (EUFA) and leaf aqueous (EULA) extracts focusing on the modulatory influence of xenobiotic metabolizing enzymes (XMEs), antioxidant enzymes, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), lactate dehydrogenase (LDH) activity, lipid peroxidation (LP), sulfhydryl groups were investigated in the hepatic and extrahepatic organs of Swiss albino mice (50 and 100 mg/kg body wt given orally for 14 days) and compared with BHA (0.75 % in diet). The modulatory and chemopreventive properties of two different doses EUFA and EULA were observed for cytochrome P450, cytochrome b5, sulfhydryl groups, NADPH-cytochrome P450 reductase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Elaeagnus umbellata Thunb; Chemopreventive properties; Xenobiotic metabolizing enzymes; Antioxidant enzymes; Lipid peroxidation; Glutathione; Lactate dehydrogenase; Glucose-6-phosphate dehydrogenase; 6-Phosphogluconate dehydrogenase.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300625 |
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| Registros recuperados: 29 | |
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