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Registros recuperados: 210 | |
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GALLEGO RÚA,Adriana María; HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía. |
In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Histology; Reserve accumulation; Recalcitrance; Somatic embryogenesis. |
Ano: 2016 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200002 |
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Maciel, Simone Alencar; Universidade Federal do Acre; Fermino Junior, Paulo Cesar Poeta; Universidade Federal do Acre; Silva, Ricardo Alexandre; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Scherwinski-Pereira, Jonny Everson; Embrapa Recursos Genéticos e Biotecnologia. |
The objective of this study was to morpho-anatomically characterize nodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 µM) and BAP (0, 5, 10 µM). After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 µM). After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 µM), where the presence of multi-granular structures were observed.... |
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Palavras-chave: Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos 5.00.00.00-4 Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos Ciências Agrárias. |
Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3248 |
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Figueiredo Júnior, Jalceyr Pessoa; Costa, Fernando Guilherme Perazzo; Guerra, Ricardo Romão; Santana, Marcelo Helder Medeiros; Lima, Matheus Ramalho de; Pinheiro, Sarah Gomes. |
An experiment was carried out to evaluate the requirement of digestible methionine for growing pullets at growth phase (7 to 12 weeks of age). A completely randomized design was distributed in five treatments, six replicates, and 15 pullets per experimental unit. 450 Dekalb White pullets from the 7th weeks of age, with an average initial weight of 313.14 ± 12.49 g were used. Dietary treatments consisted in five diets supplemented with DL-Methionine which resulted in five levels of digestible methionine (0.266, 0.294, 0.322, 0.350, and 0.378 %). Performance, serological blood, histopathology and histomorphometry data were evaluated. Quadratic responses were observed for final live weight (p < 0.0143), weight gain (p < 0.0073), feed conversion... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic profile Essential amino acid Histology Morphometry. enzymatic profile; Essential amino acid; Histology; Morphometry.. |
Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47222 |
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Cruz, Zilma Maria Almeida; Universidade Vila Velha; Castro, Rodrigo Roque Lesqueves de; Universidade Vila Velha; Canal, Adriana; Azevedo Júnior, Romildo Rocha; Universidade Vila Velha; Silva, Ary Gomes da; Universidade Vila Velha; Ramos, Alessandro; Universidade Vila Velha. |
The effects of polluted water at three sites in the Marinho River, Brazil, on Oreochromis niloticus (Nile tilápia) were investigated using histological, hematological and biochemical approaches. Fish exposed to the impacted water demonstrated that histological changes in gills were accompanied by nuclear and micronuclei abnormalities in cells. The activity of liver and plasma biomarkers (alkaline phosphatase (ALP), acid phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver glutathione S-transferase (GST)) showed an expressive change due to the. The results were also correlated with the highest levels of Cu+2, Zn+2 and Mn+2 in the water. The data of this study evidenced the importance of using a set of biomarkers to... |
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Palavras-chave: Aminotransferases; Phosphatases; Glutathione-S-transferase; Histology; Cell abnormality; Tilapia. |
Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13207 |
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Machado-Santos, Clarice; Universidade Federal da Bahia.; Teixeira, Monalisa Jales; Médica Veterinária Autônoma; Sales, Armando; Universidade Federal Rural do Rio de Janeiro; Abidu-Figueiredo, Marcelo; Universidade Federal Rural do Rio de Janeiro. |
The broad-snouted caiman, Caiman latirostris, is widely distributed in countries of South America. In Brazil it is considered an endangered species because of natural habitat destruction and illegal hunting. In reptiles, the thyroid gland plays an integral part in ecdysis, reproduction, tail regeneration, growth, endocrine function and metabolism rate. The aim of the present study was to characterize the thyroid gland morphology of C. latirostris, based on histochemical and immunohistochemical techniques. The thyroid glands were fixed in Bouin's fluid and serial cross sections were stained with hematoxylin-eosin, Mallory’s trichrome, periodic acid-Schiff (PAS) and Alcian blue (AB pH 1.5 and 2.5). The immunohistochemical technique for 5-HT-IR cells was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências biológicas Thyroid; Histology; Reptiles; 5-HT Histologia. |
Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444 |
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REIS,TATIANE C.; SOARES,MARCUS A.; SANTOS,JOSÉ B. DOS; SANTOS,CONCEIÇÃO A. DOS; SERRÃO,JOSE EDUARDO; ZANUNCIO,JOSÉ C.; FERREIRA,EVANDER A.. |
Abstract Herbicides can impact non-target metabolic pathways in natural enemies and lead to the reduction of these populations in the field. Behavioral characteristics, morphology and histology of reproductive structures and reproduction of females of Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae) were evaluated under the effect of the herbicides atrazine, nicosulfuron and the mixture of both. The number of mature oocytes per ovary was lower in females exposed to the herbicides atrazine, nicosulfuron and the mixture of both. Herbicides did not affect the longevity and mortality of P. nigrispinus, therefore, they are selective for this predator. On the other hand, herbicides can cause sublethal effects by affecting the reproduction of predators. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Behavior; Histology; Morphology; Reproduction; Herbicides. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703625 |
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BECEGATO,ESTELLA Z.; ANDRADE,JANAINA P.; GUIMARÃES,JULIANA P.; VERGARA-PARENTE,JOCIERY E.; MIGLINO,MARIA ANGELICA; SILVA,FERNANDA M. DE OLIVEIRA E. |
The reproductive morphology of cetaceans is poorly studied and, despite the large number of strandings, reports on this subject are scarce due to access to carcasses mostly in an advanced state of decomposition. The present study aimed to describe histological characteristics of the female genital tract ofSotalia guianensis, in order to assist in future studies on the reproductive biology of these animals. Females of different ages, from stranding events on beaches in northeastern Brazil, were used. Fragments of all organs were collected and processed for light and scanning electron microscopy. Histological analyses showed that these structures were similar to those found in terrestrial mammals, with some peculiarities, such as the presence of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Female genital system; Anatomy; Histology; Guiana dolphin. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401727 |
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SANTOS,CAROLYNE D.S.; BALBO,SANDRA L.; GUIMARÃES,ANA T.B.; SAGAE,SARA C.; NEGRETTI,FÁBIO; GRASSIOLLI,SABRINA. |
ABSTRACT Here, we evaluated whether the exposure of rats to a cafeteria diet pre- and/or post-weaning, alters histological characteristics in the White Adipose Tissue (WAT), Brown Adipose Tissue (BAT), and liver of adult male offspring. Female Wistar rats were divided into Control (CTL; fed on standard rodent chow) and Cafeteria (CAF; fed with the cafeteria diet throughout life, including pregnancy and lactation). After birth, only male offspring (F1) were maintained and received the CTL or CAF diets; originating four experimental groups: CTL-CTLF1; CTL-CAFF1; CAF-CTLF1; CAF-CAFF1. Data of biometrics, metabolic parameters, liver, BAT and WAT histology were assessed and integrated using the Principal Component Analysis (PCA). According to PCA analysis worse... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Obesity; Dams; Offspring; Histology. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602887 |
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AZEVEDO,JORGE F. DE; HERMES-ULIANA,CATCHIA; LIMA,DIRLENE P.; SANT'ANA,DÉBORA M.G.; ALVES,GILBERTO; ARAÚJO,EDUARDO J.A.. |
This study sought to morphometrically analyze the jejunal wall of protein-malnourished rats administered a probiotic supplement. The sample consisted of recently weaned Wistar rats (Rattus norvegicus) distributed among four groups: animals given a commercial diet (G1, n = 4); animals given the same ration as G1 plus a probiotic supplement (G2, n = 4); animals given a 4% protein diet (G3, n = 4); and animals given the same ration as G3 plus a probiotic supplement (G4, n = 4). After 12 weeks, part of the jejunum was harvested and subjected to routine histological processing. Transverse sections with a thickness of 3 µm were stained with HE, and histochemical techniques were used to assay for glycoconjugates, including staining with periodic acid-Schiff (PAS)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bowel; Digestive system; Histology; Morphometry; Protein-energy malnutrition; Small intestine. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301303 |
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SILVA,PAULO R.D. DA; STEFENON,VALDIR M.. |
The plant species occurring in the savanna region of the Cerrado biome in Brazil present typical morphological and physiological adaptations to a dry climate with seasonal occurrence of wildfires. In this study, the histological features of the root system, the main sites of synthesis and storage of starch and the initial phases of the bud development were characterized inJacaranda ulei. The anatomical features observed in the root system of J. ulei are related to the needs of the species to survive in the Cerrado. The histochemical analyses demonstrated high synthesis of glucose and glycoprotein after the third day of in vitro culture, in the proximal cells of the cortical parenchyma of the exoderm. Meristematic primordia were observed in the ninth day... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado Biome; Histology; Savanna; Wildfire. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000100271 |
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Lima,Dirlene P.; Azevedo,Jorge F. de; Hermes-Uliana,Catchia; Alves,Gilberto; Sant'ana,Débora M.G.; Araújo,Eduardo J.A.. |
The objective of this study was to analyze morphometrically the colon wall strata of malnourished rats supplemented with probiotics. Sixteen recently weaned Wistar rats (Rattus norvegicus) were distributed into four groups: animals that received commercial chow (G1, n = 4); animals that received the same feed as G1 and were supplemented with probiotics (G2, n = 4); animals that received chow with 4% of proteins (G3, n = 4); animals that received the same feed as G3 and were supplemented with probiotics (G4, n = 4). After 12 weeks, the proximal colon was collected and submitted to histological processing. Three-µm cuts were stained with H.E., Periodic Acid Schifff (P.A.S.) + diasthasis solution and Alcian Blue (A.B.) pH 2.5 and pH 1.0. The morphometric... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Histology; Large intestine; Malnourishment; Morphometry; Probiotics. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300015 |
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PAULINI,FERNANDA; CHAVES,SACHA B.; RÔLO,JOSÉ LUIZ J.P.; AZEVEDO,RICARDO B. DE; LUCCI,CAROLINA M.. |
ABSTRACT Visualization and clear understanding of the ovarian structures are important in determining the stage of oestrus, helping to diagnose several pathologies and supporting advances in reproductive technologies. In this research, computerized microtomography (microCT) was used to explore and characterize the ovarian structure of seven mammalian species. Ovaries of rats, female dog, queens, cows, mares, sows and a female donkey were used. After microCT scanning, the same samples were prepared for histologic evaluation, used here as a validation criterion. It was possible to distinguish regions of the cortex and medulla, visualize the morphology and distribution of blood vessels, clearly observe corpus luteum and antral follicles, and visualize oocytes... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovarian follicle; Blood vessels; Histology; Tomography; Ovary. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502131 |
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Registros recuperados: 210 | |
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