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| Registros recuperados: 36 | |
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| Silveira, Márcio José da; UEM; Teixeira, Gustavo Monteiro; UNESP/Botucatu; Oliveira, Edson Fontes de; UEM. |
| Laboratórios de Zoologia em Instituições de Pesquisa e Ensino têm demonstrado grande demanda por peças anatômicas para utilização em aulas práticas. No entanto, embora a bibliografia voltada para este tema não seja escassa, são raras as análises das relações custo/benefício de tais processos levando em consideração aspectos técnicos, como tempo de preparo, qualidade das peças e recursos materiais e humanos. Além de técnicas usualmente utilizadas, novos produtos de baixo custo, não-citados na literatura e encontrados facilmente no mercado, também foram testados. O principal objetivo deste trabalho foi a elaboração e organização de informações sobre técnicas alternativas de maceração de peças ósseas para coleções didáticas, realizando análises comparativas... |
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Palavras-chave: Maceração; Clarificação de esqueletos; Peróxido de hidrogênio; Suco de mamão maceration; Skeleton clarification; Hydrogen peroxide; Papaya juice. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5876 |
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| Yoon,Jung Mi; Koppula,Sushruta; Huh,Se Jong; Hur,Sun Jin; Kim,Chan Gil. |
| BACKGROUND: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 μg/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. METHODS: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. RESULTS AND CONCLUSION: In the present findings we showed that low concentration of DC (<2.0 μg/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells... |
| Tipo: Journal article |
Palavras-chave: Apoptosis; Caspase; Cisplatin; FasL; Hydrogen peroxide; Tetracycline. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100038 |
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| Matsuguma,Luciana Shizue; Lacerda,Luiz Gustavo; Schnitzler,Egon; Carvalho Filho,Marco Aurélio da Silva; Franco,Célia Maria Landi; Demiate,Ivo Mottin. |
| Two commercial varieties of Peruvian carrot ('Amarela de Carandaí' and 'Senador Amaral') were processed into flour, starch and bagasse and chemically evaluated. The starch was extracted, modified with H2O2 and characterized by the physicochemical methods. By using the methylene blue dyeing, the granules of the modified starches showed intense blue color. The carboxyl content, the reducing power and the amount of the water liberated from the pastes after the freeze-thawing were higher for the oxidized starches and their pastes were clearer than those of the native starches of the two varieties from the two production areas. The RVA viscoamylography showed that the modified starches had lower viscosities with differences between the varieties. In the thermal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peruvian carrot varieties; Starch; Hydrogen peroxide; Technological properties. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300022 |
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| Estivallet,Hidê Virgínia; Rodrigues,Maria Inês; Marroni,Norma Possa. |
| This work investigated the frog gastric mucosa response to hydrogen peroxide and ethanol induced injury. Acid and mucus secretion were estimated "in vitro" in control animals with intragastric absolute ethanol (1ml/30min. or 2ml/60min.) and hydrogen peroxide. The gastric mucosa morphological conditions were assessed "in vivo", concerning lesion area, pH and mucus. Ethanol (1ml/30min.) was observed to cause hyperemia, cell damage, rupture, edema, erosions, necrosis in gastric mucosa and significant increase in acid secretion. Absolute ethanol (2ml/60min.) caused a decrease in acid secretion due to alcalinization and an increase of mucus and pH. Intragastric hydrogem peroxide provoked gastric unwrinkling and hyperemia, acid secretions were not increased,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gastric acid secretion; Mucus; Ethanol; Hydrogen peroxide; Frog. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100007 |
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| Arruda,Maria Sueli Parreira de; Correa,Mara Adriana; Venturini,James; Félix,Maira Camila; Rosis,Ana Maria Bormio De; Galhiane,Mário Sérgio; Chierice,Gilberto Orivaldo; Costa,Maria Renata Sales Nogueira. |
| The aim of the present study was to examine the effect of a diet rich in synthetic PEtn on the metabolism macrophages of tumor-bearing mice. The results demonstrated that PEtn increased the animals' survival time. In addition, the treated animals released smaller amounts of hydrogen peroxide (H2O2) and nitric oxide (NO) than the non-treated animals, particularly after day 14. From the results it could be concluded that H2O2 and NO were important in the modulation of neoplastic growth, and pointed to a promising role of PEtn in the control of human neoplasms. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phosphoethanolamine; Ehrlich tumor; Nitric oxide; Hydrogen peroxide; Lipids. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600016 |
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| Branco,K.M.G.R.; Nardi,R.M.D.; Moreira,J.L.S.; Nunes,A.C.; Farias,L.M.; Nicoli,J.R.; Carvalho,M.A.R.. |
| Lactobacilli isolated from the vaginal tract of women with and without bacterial vaginosis (BV) were identified and characterized for the production of antagonists. Bacterial samples were isolated from healthy women (N = 16), from patients with clinical complaints but without BV (N = 30), and from patients with BV (N = 32). Identification was performed using amplified ribosomal DNA restriction analysis. Production of antagonistic compounds was evaluated by the double-layer diffusion technique using Gram-positive (N = 9) and Gram-negative bacteria (N = 6) as well as yeast (N = 5) as indicator strains. Of a total of 147 isolates, 133 were identified as pertaining to the genus Lactobacillus. Lactobacillus crispatus was the species most frequently recovered,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus; Bacterial vaginosis; Antagonism; Hydrogen peroxide; Identification. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400003 |
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| Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.. |
| Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398 |
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| Silveira,E.M.S.; Kroth,A.; Santos,M.C.Q.; Silva,T.C.B.; Silveira,D.; Riffel,A.P.K.; Scheid,T.; Trapp,M.; Partata,W.A.. |
| The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Superoxide anion generation; Hydrogen peroxide; Total thiol content; Lipid hydroperoxide levels; Total antioxidant capacity; Superoxide dismutase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700608 |
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| Riffel,A.P.K.; Santos,M.C.Q.; de Souza,J.A.; Scheid,T.; Horst,A.; Kolberg,C.; Belló-Klein,A.; Partata,W.A.. |
| Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Total thiol; Superoxide anion generation; Nitric oxide; Lipid hydroperoxides; Hydrogen peroxide; Total antioxidant capacity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400612 |
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| Belló-Klein,A.; Oliveira,A.R.; Miranda,M.F.S.; Irigoyen,M.C.; Homem-de-Bittencourt Jr.,P.I.; Llesuy,S.; Belló,A.A.. |
| Hydrogen peroxide (H2O2) perfused into the aorta of the isolated rat heart induces a positive inotropic effect, with cardiac arrhythmia such as extrasystolic potentiation or cardiac contractures, depending on the dose. The last effect is similar to the "stone heart" observed in reperfusion injury and may be ascribed to lipoperoxidation (LPO) of the membrane lipids, to protein damage, to reduction of the ATP level, to enzymatic alterations and to cardioactive compounds liberated by LPO. These effects may result in calcium overload of the cardiac fibers and contracture ("stone heart"). Hearts from male Wistar rats (300-350 g) were perfused at 31oC with Tyrode, 0.2 mM trolox C, 256 mM H2O2 or trolox C + H2O2. Cardiac contractures (baseline elevation of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trolox C; "stone heart"; Free radicals; Hydrogen peroxide. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100013 |
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| Zhang,Hongbin; Yuan,Bo; Huang,Hanfei; Qu,Siming; Yang,Shikun; Zeng,Zhong. |
| Nuclear factor erythroid-related factor 2 (Nrf2) has been implicated in several detoxifying and antioxidant defense processes. Nrf2-mediated heme oxygenase-1 (HO-1) expression was demonstrated to play a key role against oxidative stress. Gastrodin (GSTD) is a well-known active compound isolated from the roots of Rhizoma gastrodiae, a plant used in ancient Chinese traditional medicine. The aim of this work was to investigate whether GSTD could alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells (LSECs). In LSECs exposed to 1 mM H2O2, treatment with GSTD (1, 10, or 50 µM) resulted in higher cell viability than the untreated control. Treated cells maintained a higher Bcl2/Bax ratio and suppressed caspase-9 expression compared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gastrodin; Liver sinusoidal endothelial cells; Heme oxygenase 1; Oxidative stress; Hydrogen peroxide. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000611 |
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| Rao,F.; Deng,C.Y.; Zhang,Q.H.; Xue,Y.M.; Xiao,D.Z.; Kuang,S.J.; Lin,Q.X.; Shan,Z.X.; Liu,X.Y.; Zhu,J.N.; Yu,X.Y.; Wu,S.L.. |
| Macrophage migration inhibitory factor (MIF), a pleiotropic cytokine, plays an important role in the pathogenesis of atrial fibrillation; however, the upstream regulation of MIF in atrial myocytes remains unclear. In the present study, we investigated whether and how MIF is regulated in response to the renin-angiotensin system and oxidative stress in atrium myocytes (HL-1 cells). MIF protein and mRNA levels in HL-1 cells were assayed using immunofluorescence, real-time PCR, and Western blot. The result indicated that MIF was expressed in the cytoplasm of HL-1 cells. Hydrogen peroxide (H2O2), but not angiotensin II, stimulated MIF expression in HL-1 cells. H2O2-induced MIF protein and gene levels increased in a dose-dependent manner and were completely... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Macrophage migration inhibitory factor; HL-1 cells; Hydrogen peroxide; Atrial fibrillation; Protein kinases. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900746 |
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| Registros recuperados: 36 | |
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