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| Registros recuperados: 13 | |
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| REYES,EUGENIO; BARAHONA,SALVADOR; FISCHMAN,OLGA; NIKLITSCHEK,MAURICIO; BAEZA,MARCELO; CIFUENTES,VÍCTOR. |
| In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the... |
| Tipo: Journal article |
Palavras-chave: Pichia anomala; 5.8S rDNA; RAPD; Internal transcribed spacer; Antifungal drugs. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004 |
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| Silva-Pinhati,A.C.O.; Bacci Jr.,M.; Hinkle,G.; Sogin,M.L.; Pagnocca,F.C.; Martins,V.G.; Bueno,O.C.; Hebling,M.J.A.. |
| Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atta; Acromyrmex; Internal transcribed spacer; Leaf-cutting ants; Leucoagaricus; Leucoagaricus gongylophorus. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000004 |
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| Feng,Tu; Liu,Shuang; He,Xing-jin. |
| Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight... |
| Tipo: Journal article |
Palavras-chave: Angelica sinensis; Internal transcribed spacer; Molecular authentication; Specific primers; Traditional Chinese medicine. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100009 |
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| Vivas,Caio Vinicius; Moraes,Ramiris César Souza; Alves-Araújo,Anderson; Alves,Marccus; Mariano-Neto,Eduardo; van den Berg,Cássio; Gaiott,Fernanda Amato. |
| The Atlantic Forest is a phytogeographic domain with a high rate of endemism and large species diversity. The Sapotaceae is a botanical family for which species identification in the Atlantic Forest is difficult. An approach that facilitates species identification in the Sapotaceae is urgently needed because this family includes threatened species and valuable timber species. In this context, DNA barcoding could provide an important tool for identifying species in the Atlantic Forest. In this work, we evaluated four plant barcode markers (matK, rbcL, trnH-psbA and the nuclear ribosomal internal transcribed spacer region -ITS) in 80 samples from 26 species of Sapotaceae that occur in the Atlantic Forest. ITS yielded the highest average interspecific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Internal transcribed spacer; Taxonomy; Tree species; Tropical forest. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500009 |
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| Escobedo-fregoso, C.; Arzul, Isabelle; Carrasco, Noelia; Gutierrez-rivera, J. N.; Llera-herrera, Raul; Vazquez-juarez, R.. |
| Prevalence of the protozoan Perkinsus spp. in the gills of the pleasure oyster Crassostrea corteziensis from two estuaries in Nayarit, Mexico, was measured. The protozoan was identified by PCR amplification of the internal transcribed spacer (ITS) region of the rDNA of Perkinsus spp. The pathogen was found in 92% of oysters from Boca de Camichín and 77% of oysters from Pozo Chino. ITS sequences characterized from C. corteziensis showed 96–100% similarity to Perkinsus marinus. The most frequent ITS sequence, (GenBank JQ266236) had 100% identity with the ITS locus of P. marinus from New Jersey, Maryland, South Carolina, and Texas, and the second most frequent observed sequence (GenBank JQ266240) was 100% identical to ITS sequences of P. marinus from New... |
| Tipo: Text |
Palavras-chave: Crassostrea corteziensis; Perkinsus marinus; Internal transcribed spacer; Non-internal transcribed spacer; Aquatic disease. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00137/24811/22892.pdf |
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| Shinn, A.P.; Banks, B.A.; Tange, N.; Bron, J.E.; Sommerville, C.; Aoki, T.; Wootten, R.. |
| Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon ( Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Sea louse; Copepod; Crustacea; Atlantic salmon; Sequencing; 18S ribosomal DNA; Internal transcribed spacer; Population genetics. |
| Ano: 2000 |
URL: http://www.repository.naturalis.nl/record/534343 |
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| Hyde, K.D.; Chomnunti, P.; Crous, P.W.; Groenewald, J.Z.; Damm, U.; Ko Ko, T.W.; Shivas, R.G.; Summerell, B.A.; Tan, Y.P.. |
| Several filamentous oomycete species of the genus Halophytophthora have recently been described from marine environments, mostly from subtropical and tropical ecosystems. During a survey of oomycetes from leaf litter of Spartina alterniflora in salt marshes of southeastern Georgia, isolates of four taxa were recovered that bore similarity to some members of Halophytophthora but were highly divergent from isolates of Halophytophthora s.str. based on a combined sequence analysis of two nuclear loci. In phylogenetic analyses, these isolates were placed basal to a monophyletic group comprised of Pythium of the Pythiaceae and the Peronosporaceae. Sequence and morphology of these taxa diverged from the type species Halophytophthora vesicula, which was placed... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Internal transcribed spacer; Nuclear ribosomal large subunit (nrLSU); Peronosporales; Phylogeny; Pythiaceae. |
| Ano: 2010 |
URL: http://www.repository.naturalis.nl/record/532294 |
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| Wagner, L.; Stielow, B.; Hoffmann, K.; Petkovits, T.; Papp, T.; Vágvölgyi, C.; Hoog, G.S. de; Verkley, G.; Voigt, K.. |
| The basal fungal order Mortierellales constitutes one of the largest orders in the basal lineages. This group consists of one family and six genera. Most species are saprobic soil inhabiting fungi with the ability of diverse biotransformations or the accumulation of unsaturated fatty acids, making them attractive for biotechnological applications. Only few studies exist aiming at the revelation of the evolutionary relationships of this interesting fungal group. This study includes the largest dataset of LSU and ITS sequences for more than 400 specimens containing 63 type or reference strains. Based on a LSU phylogram, fungal groups were defined and evaluated using ITS sequences and morphological features. Traditional morphology-based classification schemes... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Internal transcribed spacer; Large subunit ribosomal DNA; Taxonomic revision; Zygomycetes; Zygomycota. |
| Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/531904 |
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| Registros recuperados: 13 | |
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