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| Registros recuperados: 28 | |
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| Nascimento,Diego S.; Valasques Junior,Gildomar; Fernandes,Pedro; Ribeiro,Geise C.A.; Lima,Danyo M.; Góes-Neto,Aristóteles; Oliveira,Rodrigo Q.; Figueiredo-Ribeiro,Rita de Cassia L.; Assis,Sandra A. de. |
| Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulin; Inulinase; Yeasts; Semi-arid; Fungal endophyte. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014 |
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| Amarante,Marla Karine; Ariza,Carolina Batista; Oliveira,Carlos Eduardo Coral de; Gualtieri,Karina de Almeida; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara. |
| ABSTRACT Inulin is an effective prebiotic and its potential in modulating systemic immunity have been proposed. A subpopulation of T cells, named T regulatory cells (Tregs), expressing the Forkhead boxP3 transcription factor are key mediators of peripheral tolerance and suppress undesirable immune responses. These Tregs can be induced by cytokine transforming growth factor beta (TGF-β) and interleukin 10 (IL-10). This work aimed to evaluate inulin effects on human peripheral blood mononuclear cells (PBMC) in vitro. PBMC were incubated with inulin, and the expression of TGF-(1, FOXP3 and IL-10 was analyzed. Increased supernatant IL-10 levels were observed in PBMC of inulin-treated group (p=0.03). Moreover, FOXP3 gene expression was 7.6 fold higher in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulin; FOXP3; TGF-β1; IL-10; PBMC. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100417 |
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| Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens. |
| Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003 |
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| De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha; Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner. |
| ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587 |
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| Hao,Zhikui; Cai,Yujie; Liao,Xiangru; Zhang,Xiaoli; Fang,Zhiyou; Zhang,Dabing. |
| The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Medium optimization; Response surface method; Inulin; Chitinolyticbacter meiyuanensis SYBC-H1. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019 |
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| Samanta,A.K.; Jayapal,Natasha; Senani,S.; Kolte,A.P.; Sridhar,Manpal. |
| In recent years, there has been a growing appreciation on the relevance of gastrointestinal microflora in both ruminants and non-ruminants owing to revelation of their role in several physiological functions including digestion, nutrient utilization, pathogen exclusion, gastrointestinal development, immunity system, gut gene expression and quality of animal products. The ban imposed on the use of antibiotics and hormones in feed has compelled animal researchers in finding an alternative which could overcome the issues of conventional feed additives. Though the concept of prebiotic was evolved keeping in mind the gastrointestinal flora of human beings, presently animal researchers are exploring the efficiency of prebiotic (inulin) for modulating the gut... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prebiotic; Inulin; Mechanism of action; Livestock. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100001 |
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| VILLALVA,Fernando Josué; CRAVERO BRUNERI,Andrea Paula; VINDEROLA,Gabriel; GONÇALVEZ DE OLIVEIRA,Enzo; PAZ,Noelia Fernanda; RAMÓN,Adriana Noemí. |
| Abstract Today’s population increasingly demands and consumes healthy products. For this reason, the food industry has been developing and marketing food with added bioactive components. The aim of this work was to formulate a peach ice cream reduced in calories with an added probiotic (Bifidobacterium lactis Bb-12) and prebiotics (inulin), and to evaluate its sensory quality and acceptability as potential symbiotic food. The moisture content was 76.47%; 7.14% protein; 0.15% fat; 6.37%; carbohydrates; 9.87% inulin; 1.22% ash; 0.201% calcium, 0.155% phosphorus and 0.168% sodium. On the first and 21th day of storage counts of B. lactis Bb – 12 was 4 x 108 CFU/mL and 1.5 x 107 CFU/mL, respectively. It was possible to formulate a peach ice cream reduced in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ice cream; Inulin; Probiotic; Prebiotic; Symbiotic. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300456 |
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| CHAIYASUT,Chaiyavat; PATTANANANDECHA,Thanawat; SIRILUN,Sasithorn; SUWANNALERT,Prasit; PEERAJAN,Sartjin; SIVAMARUTHI,Bhagavathi Sundaram. |
| Abstract Host microbiome and metabolome are associated with the incidence of colorectal cancer (CC), one of the major health problems in developed countries. The pro and prebiotic supplementation helps to improve the host health. Inulin is one such prebiotic used for the enhancement of naïve probiotic bacterial population. This paper explains the impact of inulin (PRE) extracted from Jerusalem artichoke (JA), Lactobacillus plantarum HII11 (PRO), and synbiotic (SYN; inulin + L. plantarum HII11) preparation on Azoxymethane mediated CC induced rat model with respect to changes in microbial load, microbial enzymes, and preneoplastic aberrant crypt foci. The results suggested that the PRE and SYN supplementation effectively reduced the selected pathogenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulin; L. plantarum; Probiotic; Prebiotic; Synbiotic. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000200328 |
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| Paz,Noelia Fernanda; Oliveira,Enzo Gonçalvez de; Kairuz,Martha Susana Nuñez de; Ramón,Adriana Noemí. |
| Combining prebiotics and probiotic microorganisms improve quality in the formulation of foods. In this paper, the characteristics of goat milk and symbiotic yogurt were studied. Raw goat milk was analyzed and the skimming process was optimized. For the formulation of a potentially non-fat symbiotic yogurt made with skimmed goat milk, inulin, gelatin, sugar, and Streptococcus salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei subsp. rhamnoshus. Chemical characteristics, acceptability, and viability of lactic acid bacteria and probiotic culture were assessed. The protein and fat content of the raw milk was 2.90 and 3.56 g/100 mL, respectively. The optimum skimming process was obtained at 9,800 rpm and 4 °C for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat milk; Yogurt; Symbiotic; L. casei; Inulin. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300029 |
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| Registros recuperados: 28 | |
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