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| Registros recuperados: 62 | |
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| SIMÃO,ANDERSON A.; MARQUES,TAMARA R.; MARCUSSI,SILVANA; CORRÊA,ANGELITA D.. |
| ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicinal plants; Obesity; Α-amylase; Α-glycosidase; Lipase; Trypsin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502155 |
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| Ferreira,V.P.A.; Ferreira,W.M.; E.O.S.,Saliba; Teixeira,A.O.. |
| Analisou-se a atividade enzimática in vitro de uma lipase em diferentes condições de temperatura (simulando o processo de peletização) e pH (acidez gástrica) e na presença de íons minerais e metálicos. Para avaliar a estabilidade térmica, a enzima foi incubada a 80(0)C por quatro tempos (0, 5, 10 e 15 minutos). O efeito do pH foi estudado submetendo-se a enzima a quatro valores de pH (7,3, 2,0, 3,2 e 5,0). Para avaliar o efeito da lipase na presença de íons minerais e metálicos, incubou-se a enzima em diferentes soluções contendo cálcio, cobre, cobalto, manganês e ferro. Frente às diferentes condições, a lipase conservou atividade parcial, mas não pôde ser considerada uma enzima ideal em função do efeito inibidor promovido pelo pH ácido e pelas soluções... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atividade; Lipase; Íons; PH; Temperatura. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300011 |
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| Cançado,S.V.; Baião,N.C.. |
| Os objetivos deste trabalho foram determinar o efeito de três períodos de jejum (zero, 24 e 48 horas) entre o nascimento e o alojamento de pintos e da adição de óleo à ração sobre o crescimento alométrico dos órgãos digestivos e estudar a atividade da lipase no pâncreas e intestino delgado. Foram utilizados 720 pintos de corte machos, criados de um a 15 dias de alojamento. As avaliações do crescimento dos órgãos digestivos foram feitas nos dias zero, três, seis, nove, 12 e 15 de idade. As avaliações da concentração de lipase no pâncreas foram feitas nos dias zero, três, seis, nove, 12 e 15, e no intestino delgado nos dias zero, três e seis. O delineamento experimental foi em arranjo fatorial 3 x 2 (três períodos de jejum e dois tipos de ração). O... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pintos de corte; Trato gastrintestinal; Lipase; Jejum. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600011 |
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| Santos,M.V.; Oliveira,C.A.F.; Augusto,L.F.B.; Aquino,A.A.. |
| Estimou-se a origem da atividade lipolítica do leite com contagens de células somáticas (CCS) ajustadas para diferentes níveis. A partir de lotes de leite cru com baixa (100.000 cel/ml) e alta (1.000.000 cel/ml) CCS, formaram-se três tratamentos: A) leite com baixa CCS; B) leite com alta CCS; C) leite originalmente com baixa CCS e com adição de células somáticas. O leite foi submetido à pasteurização e armazenado por 21 dias sob refrigeração a 6ºC. Durante o período de armazenamento, foram coletadas amostras nos dias 1, 7, 14 e 21 para avaliação da concentração de ácidos graxos livres (AGL). Não foi observado efeito de tratamento sobre a concentração de AGL, durante todo o período de armazenamento e nem em cada dia de coleta isoladamente, cujas médias de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite; Células somáticas; Atividade enzimática; Lipase. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400002 |
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| Katoch,Meenu; Salgotra,Aseem; Singh,Gurpreet. |
| This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacopa monnieri; Antimicrobial; ITS 5.8S rDNA; Lipase; Amylase; Endophyte. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500714 |
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| Sugahara,Vanessa Hitomi; Varéa,Geni da Silva. |
| Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842 |
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| Kumari,Annapurna; Mahapatra,Paramita; Banerjee,Rintu. |
| Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Central composite design; Enterobacter aerogenes; Lipase; Optimization; Parameter; Response Surface Methodology. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005 |
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| Zhang,Qiaoyan; Qian,Junqing; Ma,Lingzhi. |
| This work aimed to obtain an ideal mutant strain with higher lipase yield using hydrazine hydrate (HZH) as a novel additive to treat Flavobacterium sp. strain YY25 by supercritical CO2. The survival rate and the positive mutation rate of the tested strain were strongly dependent on the dose of HZH. The treatment by 0.5% HZH in supercritical CO2 (8 MPa, 35°C) for 30 min provided 58.3% of positive mutation rate and an expected mutant strain with about 76.7% increase in lipase yield compared with the wild strain. Possible mutagenesis mechanisms were further explored. The analysis on pH drop of the treated seed liquid was performed to better understand the interaction. Results proved that the induced mutation with enhanced yield of lipase could be achieved by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Supercritical CO2; Mutation; PH Drop; Lipase; Flavobacterium sp. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400003 |
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| Sooch,Balwinder Singh; Kauldhar,Baljinder Singh. |
| The aim of the present work was to study the influence of multiple bioprocess parameters for the maximum production of lipase from Pseudomonas sp. BWS-5. The culture reached the stationary phase of growth after 36h of incubation when the maximum lipase production was obtained at flask level. The different media components such as carbon sources, nitrogen sources, trace elements and process parameters such as the pH of the medium, temperature and time of incubation, agitation/stationary conditions, etc. were optimized at flask level and at bioreactor level. The maximum enzyme production of 298 IU/mL was obtained with the use of simple medium with pH 6.5 containing glucose (1 %, w/v), peptone (3 %, w/v) and KCl (0.05 %, w/v) after 30h of incubation at 37°C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipase; Pseudomonas; Medium Optimization. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500002 |
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| Iqbal,Syeda Abeer; Rehman,Abdul. |
| ABSTRACTA lipase producing bacterium was isolated from oil contaminated effluents of various industries from Sheikhupura Road, Pakistan, and, on the basis of biochemical and 16S rRNA ribotyping, was identified asBacillus subtilis. The optimum temperature and pH for the growth of the culture were 37ºC and 7.0, respectively.B. subtilis I-4 had a lag phase of 4 h in LB medium while this phase prolonged to 6 h in oil containing medium. The optimum temperature and pH for the enzyme activity were 50ºC and 7.0, respectively. Maximum lipase activity was found in the presence of Ca ions. Olive oil and Tween 80 induced lipase gene in the bacterium while concentration of oil greater than 2% retarded the growth of the organism. In addition to lipaseB. subtilis I-4... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilisI-4; Lipase; Alkane hydroxylase. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500789 |
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| Rajendran,Aravindan; Palanisamy,Anbumathi; Thangavelu,Viruthagiri. |
| Lipases are one of the most important industrial biocatalyst which catalyzes the hydrolysis of lipids. It can also reverse the reaction at minimum water activity. Because of this pliable nature, it is widely exploited to catalyze the diverse bioconversion reactions, such as hydrolysis, esterification, interesterification, alcoholysis, acidolysis and aminolysis. The property to synthesize the esters from the fatty acids and glycerol promotes its use in various ester synthesis. The esters synthesized by lipase finds applications in numerous fields such as biodiesel production, resolution of the recemic drugs, fat and lipid modification, flavour synthesis, synthesis of enantiopure pharmaceuticals and nutraceuticals. It plays a crucial role in the food... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipase; Food applications; Ester; Esterification; Synthesis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100026 |
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| de Melo,T.S.; Lima,P.R.; Carvalho,K.M.M.B.; Fontenele,T.M.; Solon,F.R.N.; Tomé,A.R.; de Lemos,T.L.G.; da Cruz Fonseca,S.G.; Santos,F.A.; Rao,V.S.; de Queiroz,M.G.R.. |
| Previous studies have reported on the glucose and lipid-lowering effects of ferulic acid (FA) but its anti-obesity potential has not yet been firmly established. This study investigated the possible anti-obesitogenic effects of FA in mice fed a high-fat diet (HFD) for 15 weeks. To assess the antiobesity potential of FA, 32 male Swiss mice, weighing 20–25 g (n=6–8 per group) were fed a normal diet (ND) or HFD, treated orally or not with either FA (10 mg/kg) or sibutramine (10 mg/kg) for 15 weeks and at the end of this period, the body weights of animals, visceral fat accumulation, plasma levels of glucose and insulin hormone, amylase and lipase activities, the satiety hormones ghrelin and leptin, and tumor necrosis factor-α (TNF-α) and monocyte... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ferulic acid; Anti-obesity; High-fat diet; Leptin; Lipase; Tumor necrosis factor-α. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100603 |
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| Colla,Luciane Maria; Primaz,Andreiza Lazzarotto; Benedetti,Silvia; Loss,Raquel Aparecida; Lima,Marieli de; Reinehr,Christian Oliveira; Bertolin,Telma Elita; Costa,Jorge Alberto Vieira. |
| Abstract A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agricultural residues; Lipase; Optimization; Submerged bioprocess. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200461 |
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| Fregolente,Patricia B.L.; Fregolente,Leonardo V.; Maciel,Maria R.W.; Carvalho,Patricia O.. |
| Gamma-linolenic acid (GLA, 18:3, cis- 6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gamma linolenic acid; Borage oil; Lipase; Geotrichum candidum. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400004 |
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| Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo. |
| Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen. have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase, polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009 |
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| Registros recuperados: 62 | |
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