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Registros recuperados: 27 | |
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Wenzel Rodrigues, Inajá Marchizeli; Batista Filho, Antonio; Giordano, Isabella Barichello; Denadae, Bárbara Evelin; Fernandes, João Batista; Forim, Moacir Rossi. |
Stability during storage is a limiting factor in the development and use of bioinsecticides. This study aims to evaluate the compatibility of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana with components used in encapsulated formulations and to evaluate shelf-life in formulations with B. bassiana. Sodium alginate and maltodextrin, hemicellulose, and dimethyl sulfoxide solvents were evaluated. Compatibility was evaluated by mixing the components with PDA, and the parameters evaluated were vegetative growth, sporulation and viability. The formulations of fungi were prepared in various concentrations of sodium alginate and then added drop-wise to calcium chloride. To evaluate the stability, formulations were stored at 26.5, 4.0, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial control; Formulations; Entomopathogens; Encapsulation; Shelf-life; Microbiology.. |
Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32903 |
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Haas-Costa,Jucelaine; Alves,Luis Francisco Angeli; Daros,Alaxsandra Aparecida. |
Beauveria bassiana, an important insect pathogen was used to evaluate its effect on the feeding, behavior, histology of the digestory system and anatomy of Gallus domesticus. The fungus (strain Unioeste 4) was administrated orally treated with chicken food. Chicken feces were collected, and the birds observed for 28 days. Also, were evaluated the weight of the birds, as well as any sign of intoxication or pathological modification. Tissue samples were withdrawn to test lesions with the optic microscope. There was 100% of survival of the birds, and no behavior alteration or external lesion was found. The test group presented the highest weight gain (1383.9±54.4g). Viable conidia were observed in the feces until 24 h after feeding the fungus, indicating that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Microbial control; Non-target organisms; Birds. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200027 |
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Zhou,Xiang. |
Pandora neoaphidis overwintering had been investigated by monitoring its prevalence in Myzus persicae populations in open fields. Cabbage plants in field plots were weekly taken after mycosis initiation, to count and examine the living and dead aphids infected by P. neoaphidis. Based on the field data, infection levels (I) varied with field temperature (T), relative humidity (RH) and aphid count (numbers of living aphids per plant, N) over days (D), fitting well to the modified logistic equation I=0.91/[1+exp(8.5+(2.0H T H RH-20.2NI0)D)] (r²=0.897), where H T indicated daily hours of low temperature (<4°C), H RH daily hours of high air humidity (>90% RH) and I0 primary infection level. The model demonstrated the abiotic and biotic factors... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Entomopathogenic fungi; Aphid; Epizootic modeling; Entomophthorales; Microbial control. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100038 |
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Pupin,Antonio Marcos; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico; Roberts,Donald Wilson. |
Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var. majus were similar in percentages of total lipids. Tukey test for average of lipid content in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p= 1%) with the highest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipids; Fatty acids; Metarhizium anisopliae; Entomopathogenic fungi; Microbial control. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200012 |
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Destéfano,Ricardo Henry Rodrigues; Bechara,Ivanira José; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico. |
The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales) strain E9, isolated from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae), against larvae, prepupae and pupae stage and emergent adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on autoclaved (AS) and non-autoclaved (NAS) soil from typical citrus orchards in the State of São Paulo, Southeastern region of Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10) for AS and 2.52 x 10(10) for NAS. The lethal concentration... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial control; Metarhizium anisopliae; Anastrepha fraterculus; Fruitfly; Bioassay. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100018 |
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Francisco,Edimara Aparecida; Mochi,Dinalva Alves; Correia,Antônia do Carmo Barcelos; Monteiro,Antonio Carlos. |
This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beauveria bassiana; Lecanicillium lecanii; Paecilomyces fumosoroseus; Germination; Microbial control. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043 |
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Lopes,RB; Alves,SB. |
Microbial insecticides for cockroach control, such as those containing entomopathogenic fungi, may be an alternative to reduce contamination by chemicals in housing and food storage environments. Virulence of isolate ESALQ1037 belonging to the Metarhizium anisopliae complex against nymphs and adults of Blattella germanica (L.), and its infectivity following exposure of insects to a contaminated surface or to M. anisopliae-bait were determined under laboratory conditions. Estimated LD50 15 d following topical inoculation was 2.69 x 10(5) conidia per adult, whereas for nymphs the maximum mortality was lower than 50%. Baits amended with M. anisopliae conidia had no repellent effect on targets; adult mortality was inferior to 25%, and nymphs were not... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: German cockroach; Microbial control; Entomopathogenic fungi. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000300010 |
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BATISTA FILHO,ANTONIO; ALMEIDA,JOSÉ E.M.; LAMAS,CLÓVIS. |
The compatibility of entomopathogenic microorganisms with thiamethoxam and other insecticides was studied in vitro and under field conditions. The microorganisms tested were: Aschersonia aleyrodis, Bacillus thuringiensis, Baculovirus anticarsia (NPVAg), Beauveria bassiana, Hirsutella thompsonii, Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, Sporothrix insectorum and Verticillium lecanii. Two concentrations of each product were tested in the laboratory, based on the maximum and minimum recommended rates for application in the field. The products were added to specific culture medium for growth of the entomopathogens. Reproductive and vegetative growth were evaluated for fungi, and colony forming units (CFU) were evaluated for bacteria.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Insecticide; Microbial control; Compatibility. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000300017 |
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Mochi,Dinalva A.; Monteiro,Antonio C.; De Bortoli,Sergio A.; Dória,Háyda O.S.; Barbosa,José C.. |
This research intended to investigate if the presence of pesticides in the soil could affect the pathogenicity of Metarhizium anisopliae Metsch. (Sorokin) for Ceratitis capitata (Wied.) and assess the effect of conidia application as suspension or dry conidia. The fungicides chlorothalonyl and tebuconazol, the acaricide abamectin, the insecticide trichlorfon, and the herbicide ametrin were applied at the manufacturer-recommended doses. Soil samples were placed in glass flasks and were given the fungus as conidial suspension or dry. After pesticide application, 20 3rd-instar larvae were placed in the soil. The flasks were sealed with voile fabric and incubated at 27 ± 0.5ºC for nine days, until adult emergence; incubation continued for four more days at... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Fruit fly. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000300014 |
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Rohde,Cristhiane; Moino Jr,Alcides; Silva,Marco A T da; Carvalho,Fabiano D; Ferreira,Cleidson S. |
The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is considered one of the main pests that affect fruit production in the world. This insect spends part of its life cycle in the soil, making it a target for entomopathogenic nematodes. This work aimed at evaluating the influence of soil temperature and moisture on the infectivity of Heterorhabditis sp. RSC01 and Steinernema carpocapsae ALL to third-instars of C. capitata, and to compare the efficiency of these isolates at five different soil temperatures (19, 22, 25, 28, and 31°C) and three levels of relative soil moisture (100, 75, and 50% of field capacity). Ten C. capitata larvae were transferred to plastic jars (12 cm × 6 cm) containing 100 g soil, followed by the application of an aqueous... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial control; Fruit fly; Abiotic factor. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400022 |
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Neves,Pedro M.O.J.; Alves,Sérgio B.. |
The objectives of this study were to observe the external development of Metarhizium anisopliae and Beauveria bassiana on workers and soldiers of Cornitermes cumulans (Kollar) and to establish comparisons between the insect fixation techniques known as critical point and desiccator. Termite workers and soldiers inoculated with B. bassiana (447) and M. anisopliae (1037) were utilized. After inoculation, the insects were left at 25±0.5°C. In order to make observations, samples from both castes were removed at 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168h after inoculation. The external development of M. anisopliae and B. bassiana conidia on the termite C. cumulans showed that M. anisopliae and B. bassiana conidial germination occurred on several regions of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Microbial control; Insect pathogen; Termite. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000100010 |
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Kasburg,CR; Alves,LFA; Oliveira,DGP; Rohde,C. |
ABSTRACT Poultry red mite Dermanyssus gallinae is a cosmopolitan and hematophagous species commonly found in layer houses around the world. Poultry mite infestations may cause anemia, stress, low body weight and egg production, and mortality. Mite control is typically based on chemical products, but they are not effective and leave residues in eggs; therefore, alternative control methods, such as entomopathogenic fungi, need to researched. This study aimed at evaluating, in the laboratory, the activity of Brazilian isolates of entomopathogenic fungi against D. gallinae. The mites were collected from a commercial layer house and were sprayed with conidial suspensions (1 × 108 conidia/mL) of five isolates of Beauveria bassiana and Metarhizium anisopliae. All... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematophagous mites; Microbial control; Ectoparasites; Laying hens. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300457 |
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Registros recuperados: 27 | |
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