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| Registros recuperados: 25 | |
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| Martino-Roth,M.G.; Viégas,J.; Amaral,M.; Oliveira,L.; Ferreira,F.L.S.; Erdtmann,B.. |
| In this study, the micronuclei test (MNT) was applied in exfoliated cells of buccal mucosa, in order to evaluate the genotoxic risk associated with occupational exposure of mechanics, storage battery renovation workers, and car painters. For each individual, 3000 exfoliated buccal cells were analyzed. There was a significantly higher frequency of micronucleated cells (MNC) in the exposed workers than in controls. Smoking and drinking habits, age, and working time did not represent significant factors in terms of increasing the production of micronuclei (MN), when the control and the exposed groups were compared. These results allowed to conclude that the studied individuals belong to a risk group and should periodically undergo biological monitoring and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micronucleus test; Occupational exposure. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400021 |
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| Salvagni,Jaqueli; Ternus,Raquel Zeni; Fuentefria,Alexandre Meneghello. |
| The aim of this study was to assess the use of pesticides on farms located in the Lambedor River watershed in Guatambu, State of Santa Catarina, as well as to determine, by micronucleus testing, the risk of genotoxic impact. Samples from locally collected Cyprinus carpio, Hypostomus punctatus, Rhamdia quelen and Oreochromis niloticus gave evidence of a mean increase in micronuclei frequency from 6.21 to 13.78 in 1,000 erythrocytes, a clear indication of the genotoxic potenciality of pesticide residues in regional dams, and their significant contribution to local environmental contamination. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pesticides; Environment; Micronucleus test. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100021 |
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| Carvalho,Natália Cassettari de; Corrêa-Angeloni,Maria Júlia Frydberg; Leffa,Daniela Dimer; Moreira,Jeverson; Nicolau,Vanessa; Amaral,Patrícia de Aguiar; Rossatto,Ângela Erna; Andrade,Vanessa Moraes de. |
| Melissa officinalis (L.) (Lamiaceae), a plant known as the lemon balm, is native to the east Mediterranean region and west Asia. Also found in tropical countries, such as Brazil, where it is popularly known as "erva-cidreira" or "melissa", it is widely used in aqueous- or alcoholic-extract form in the treatment of various disorders. The aim was to investigate in vivo its antigenotoxicity and antimutagenicity, as well as its genotoxic/mutagenic potential through comet and micronucleus assaying. CF-1 male mice were treated with ethanolic (Mo-EE) (250 or 500 mg/kg) or aqueous (Mo-AE) (100 mg/kg) solutions of an M. officinalis extract for 2 weeks, prior to treatment with saline or Methyl methanesulfonate (MMS) doses by intraperitoneal injection. Irrespective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melissa officinalis; Comet assay; Micronucleus test; Genotoxicity; Antigenotoxicity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200021 |
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| Silva,Fernanda R. da; Erdtmann,Bernardo; Dalpiaz,Tiago; Nunes,Emilene; Ferraz,Alexandre; Martins,Tales L.C.; Dias,Johny F.; Rosa,Darlan P. da; Porawskie,Marilene; Bona,Silvia; Silva,Juliana da. |
| Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; Genotoxicity; Micronucleus test; Helix aspersa; Nicotiana tabacum; Tobacco leaves. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200018 |
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| Kvitko,Katia; Bandinelli,Eliane; Henriques,João A.P.; Heuser,Vanina D.; Rohr,Paula; Silva,Fernanda R. da; Schneider,Naye Balzan; Fernandes,Simone; Ancines,Camile; Silva,Juliana da. |
| Our mutagenesis group has been studying with important economic drivers of our state, such as agriculture, the footwear and leather industry and open-cast coal mining. Working conditions in these sectors have potentially harmful to humans. The aim of these studies is to determine the health risk of workers by biomonitoring subjects exposed to genotoxic agents. The main results of our studies with vineyard farmers we observed a high rate of MN and DNA damage in individuals exposed to pesticides (p < 0.001). In addition, some effects of genetic polymorphisms in the modulation of MN results were observed in this group. Tobacco farmers were also evaluated at different crop times. The results showed a significant increase in the Damage index and frequency in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Occupational exposure; Comet assay; Micronucleus test; Pesticide; Coal dust. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600022 |
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| Campana,Marcela Alejandra; Panzeri,Ana María; Moreno,Víctor Jorge; Dulout,Fernando Noel. |
| Pyrethroid lambda-cyhalothrin genotoxicity was evaluated using the micronucleus test in Rana catesbeiana tadpoles. The effects of concentration and exposure time on the micronuclei frequency were studied in blood smears obtained from tadpoles exposed to four concentrations (0.02, 0.1, 0.2 and 0.4 mg/L) of the compound for 24, 48, 72 and 96 h and 8, 15, 20 and 30 days. As a positive control, tadpoles were exposed to cyclophosphamide (5 mg/L). The micronucleated cell frequency was expressed per 1,000 cells. R. catesbeiana tadpoles exposed to increasing concentrations of lambda-cyhalothrin showed an increase in the micronuclei frequency in peripheral blood. Tadpoles exposed to cyclophosphamide (CP) also showed a significant increase in micronucleated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Micronucleus test; Lambda-cyhalothrin; Tadpoles. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100016 |
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| Oliveira,Rodrigo Juliano; da Cruz Leite Santos,Naiara; Pesarini,João Renato; de Oliveira,Beatriz Carneiro; Berno,Claudia Rodrigues; de Araújo,Flávio Henrique Souza; da Silveira,Ingridhy Ostaciana Maia Freitas; Nascimento,Raquel Oliveira; Brochado Antoniolli-Silva,Andréia Conceição Milan; Duenhas Monreal,Antônio Carlos; Beatriz,Adilson; de Lima,Dênis Pires; da Silva Gomes,Roberto. |
| Abstract The increased incidence of cancer and its high treatment costs have encouraged the search for new compounds to be used in adjuvant therapies for this disease. This study discloses the synthesis of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid (IR-01) and evaluates not only the action of this compound on genetic integrity, increase in splenic phagocytosis and induction of cell death but also its effects in combination with the commercial chemotherapeutic agents doxorubicin, cisplatin and cyclophosphamide. IR-01 was designed and synthesized based on two multifunctionalyzed structural fragments: 4-aminoantipyrine, an active dipyrone metabolite, described as an antioxidant and anti-inflammatory agent; and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Splenic phagocytosis; Comet assay; Micronucleus test; Cell death; Chemoprevention. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100154 |
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| Mendonça,Leonardo Meneghin; Machado,Carla da Silva; Teixeira,Cristiane Cardoso Correia; Freitas,Luis Alexandre Pedro de; Bianchi,Maria Lourdes Pires; Antunes,Lusânia Maria Greggi. |
| Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcuma longa; Antigenotoxicity; Micronucleus test; DNA damage; Comet assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490 |
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| Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E.. |
| Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643 |
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| Espósito,Anderson Victorino; Pereira,Danielli Maria Vieira; Rocha,Leandro Machado; Carvalho,José Carlos Tavares; Maistro,Edson Luis. |
| Plants of the genus Hypericum, long used in folk medicine, contain active compounds which present, anti-septic, diuretic, digestive, expectorant, vermifugal, anti-depressive and other properties. The possible clastogenic effect of a H. brasiliense extract was tested in vivo on the bone marrow cells of Wistar rats. The extract was administered by gavage at doses of 50, 150 and 300 mg/kg body weight. Experimental and control animals were submitted to euthanasia 24 h after the treatment for micronucleus (MN) and chromosome preparations. H. brasiliense extract did not induce statistically significant increases in the average numbers of MN or chromosome aberrations in the test systems employed. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hypericum brasiliense; Micronucleus test; Chromosome aberrations. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100026 |
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| Della Torre,A; Albuquerque,LBL; Farrapo,NM; Oshima-Franco,Y; Santos,MG; Tavares,RVS; Rodas,ACD; Dal Belo,CA; Cardoso,CRP; Varanda,EA; Groppo,FC; Lopes,PS. |
| Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ames test; Micronucleus test; Mutagenicity; Plant extract; Plathymenia reticulata. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 |
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| ALVES,JODEL S.; SILVA,FERNANDA R. DA; SILVA,GABRIELI F. DA; SALVADOR,MIRIAN; KVITKO,KÁTIA; ROHR,PAULA; SANTOS,CARLA E.I. DOS; DIAS,JOHNNY F.; HENRIQUES,JOÃO A.P.; SILVA,JULIANA DA. |
| ABSTRACT Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profile was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pesticides; Comet assay; Micronucleus test; Superoxide dismutase activity; PON1 gene. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100349 |
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| SPERA,KAMILLE D.; FIGUEIREDO,PATRÍCIA A.; SANTOS,PAMELA C.E; BARBOSA,FERNANDO C.; ALVES,CAIO P.; DOKKEDAL,ANNE LÍGIA; SALDANHA,LUIZ LEONARDO; SILVA,LUCIANA P.; FIGUEIREDO,CARLOS ROGÉRIO; FERREIRA,PAULO CESAR; SILVA,REGILDO M.G. DA. |
| Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: B16F10 melanoma cells; Micronucleus test; Chromosomal aberration; DPPH radical scavenging; HPLC-DAD. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617 |
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| Silva,Carolina R.; Vieira,Pabline M.; Santos,Suzana C.; Chen-Chen,Lee. |
| Duguetia furfuracea (St. Hil.) Benth & Hook f. (1862), popularly known as "sofre-do-rim-quem-quer" and "araticum-seco", is a shrub of the Annonaceae family that occurs in several regions of Brazil. In folk medicine the infusion of its leaves and twigs is used to treat rheumatism and renal colic, whereas the seed powder is mixed with water to treat pediculosis. Previous studies have described biological activities of this plant with cytotoxic, antitumoral, trypanocidal, leishmanicidal, antiplasmodial and antiprotozoal effects. In the present work, genotoxicity and cytotoxicity of Duguetia furfuracea lyophilized leaf extract were evaluated using the prophage λ induction test (SOS-Inductest) and mouse bone marrow micronucleus test. Our results showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Duguetia furfuracea; Genotoxicity; Micronucleus test; SOS-Inductest. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100015 |
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| Registros recuperados: 25 | |
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