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| Registros recuperados: 121 | |
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| Resende,Cristiano Ferrara de; Braga,Virgínia Fernandes; Silva,Cristiane Jovelina da; Pereira,Paula da Fonseca; Ribeiro,Cleberson; Salimena,Fátima Regina Gonçalves; Peixoto,Paulo Henrique Pereira. |
| This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Biodiversity conservation; Espinhaço Mountain Range. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200005 |
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| Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette. |
| Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001 |
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| Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gonçalves, Letícia de Menezes; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá. |
| No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture... |
| Tipo: Laboratory analysis |
Palavras-chave: Activated charcoal; Graphite; Orchids; Micropropagation. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257 |
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| Colombo, Larissa Abgariani; UEL; Assis, Adriane Marinho de; UEL; Faria, Ricardo Tadeu de; UEL; Roberto, Sergio Ruffo; UEL. |
| O objetivo deste trabalho foi estabelecer um protocolo para a multiplicação in vitro de Bastão-do-imperador (Etlingera elatior). Brotos axilares foram inoculados em meio MS contendo as seguintes concentrações de reguladores de crescimento durante a fase de isolamento: testemunha; BAP 2,25 mg L-1; BAP 2,25 mg L-1 + ANA 0,93 mgL-1; BAP 2,25 mg L-1 + AIA 0,87 mg L-1; BAP 4,95 mg L-1; BAP 4,95 mg L-1 + ANA 0,93 mg L-1; BAP 4,95 mg L-1 + AIA 0,87 mg L-1. As brotações provenientes desta fase foram subcultivadas em um novo meio de cultura MS: testemunha; BAP 2,25 mg L-1; BAP 3,37 mg L-1; BAP 4,50 mg L-1; BAP 2,25 mg L-1 + ANA 1,12 mg L-1; BAP 3,37 mg L-1 + ANA 2,25 mg L-1; BAP 4,50 mg L-1 + ANA 3,37 mg L-1 e avaliou-se a taxa de multiplicação dos brotos por... |
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Palavras-chave: 5.01.03.04-0 Zingiberaceae; Micropropagação; Reguladores de crescimento Produção de mudas Zingiberaceae; Micropropagation; Plant growth regulators. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4830 |
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| Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV. |
| Este trabalho teve como objetivo avaliar a resposta rizogênica in vitro de ápices caulinares de plantas jovens de mamoeiro ‘Tainung 01’, em diferentes tempos de permanência em meios de indução e regeneração. O meio de indução empregado foi o MS, suplementado com complementos orgânicos constituídos de sacarose, mio-inositol, tiamina, piridoxina, ácido nicotínico, além de AIB. O meio de regeneração diferiu do meio de indução por ser desprovido de AIB. Os tratamentos consistiram em tempos variáveis de permanência dos explantes nos meios de indução e de regeneração (indução/regeneração): 0/25; 5/20; 10/15; 15/10; 20/5 e 25/0 dias. O delineamento experimental foi inteiramente casualisado, com seis tratamentos e quatro repetições, e cada unidade experimental foi... |
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Palavras-chave: 5.01.03.04-0 Produção de Mudas Carica papaya; Auxina; Micropropagação; Cultura de tecidos Carica papaya; Auxin; Micropropagation; Tissue culture. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/863 |
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| Oliveira, Janiffe Peres de; Scherwinski-Pereira, Jonny Everson. |
| The objective of this work was to characterize the biochemically systemic bacterial isolated from banana plants, to evaluate the bacterial sensitivity to antibiotics, and to determine the phytotoxicity of banana shoots during in vitro proliferation. Systemic bacteria belonging to the Klebsiella and Aeromonas genera were isolated from the “Maravilha” (FHIA 01 AAAB), “Preciosa” (PV 4285 AAAB) and “Thap Maeo” (AAB) varieties and were then characterized. Tests of shoot sensitivity to antibiotics were performed, and the minimum inhibitory concentration (MIC) and phytotoxic effects of selected antibiotics to plants were determined. Among the 20 antibiotics evaluated, the strains showed sensitivity to cefaclor, cefalexin, cefalotin, nalidixic acid,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências agrárias Musa spp.; Micropropagação; Contaminação; Controle microbiano. Produção de mudas Musa spp.; Micropropagation; Contamination; Endophytic microorganisms; Antimicrobial control. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/27689 |
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| Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, Rosete. |
| Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliaceae; Bromeliad; Germination; Micropropagation; Seedling. Bromeliaceae bromeliad germination micropropagation seedling.. |
| Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42448 |
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| Coutinho, Ozimar de Lima; UFRR; Rego, Maílson Monteiro do; UFPB; Rego, Elizanilda Ramalho do; UFPB; Kitamura, Mario Couquiti; UFRR; Marques, Luciano Façanha; UFPB; Farias Filho, Leonildo de Paula; UFRR. |
| A murcha bacteriana, causada por Ralstonia solanacearum, é a principal doença vascular de plantas em todo o mundo. O agente causal é uma bactéria que ocorre em todas as regiões do Brasil, predominando em condições de umidade e temperatura elevadas, fatores propícios ao desenvolvimento do patógeno. Este agente causal é de difícil controle, pois sobrevive nos mais diversos hospedeiros, especialmente Solanaceas, além de outras famílias de importância alimentar, condimentar e medicinal. O uso de espécies do gênero Solanum na enxertia convencional e minienxertia em tomateiro Lycopersicon esculentum é limitada em função da incompatibilidade. Neste trabalho, utilizou-se o método da microenxertia para obtenção de plantas resistentes a doenças como a murcha... |
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Palavras-chave: 5.01.00.00-9 Agronomia Solanaceae; Murcha bacteriana; Ralstonia solanacearum; Micropropagação; Cultura in vitro 5.01.03.05-9; Solanaceae; Bacterial wilt; Ralstonia solanacearum; Micropropagation; In vitro culture. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/968 |
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| Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran. |
| In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM... |
| Tipo: Laboratory analysis |
Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 |
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| Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV. |
| Este trabalho teve como objetivo avaliar níveis de AIB no enraizamento in vitro de microestacas de mamoeiro (Carica papaya L. ‘Tainung 01’). Ápices caulinares de plantas com 120 dias de idade foram estabelecidos e avaliados em meio MS, suplementado com complementos orgânicos constituídos de sacarose a 87,64 mM, mio-inositol a 555,06 µM, tiamina-HCl a 0,30 µM, piridoxina-HCl a 2,43 µM, ácido nicotínico a 4,06 µM e 0; 0,74; 1,48; 2,96 e 5,92 µM de AIB, em delineamento inteiramente casualizado, com quatro repetições e cinco tubos de ensaio por parcela. Após 30 dias, avaliou-se a percentagem de enraizamento, o crescimento das microestacas, o comprimento da maior folha e a massa de calo. Observou-se que o maior percentual estimado de enraizamento ocorreu com a... |
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Palavras-chave: 5.01.00.00-9 Agronomia Carica papaya; Auxina; Micropropagação; Cultura de tecidos Fitotecnia Carica papaya; Auxin; Micropropagation; Tissue culture. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/866 |
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| Maciel, Simone Alencar; Universidade Federal do Acre; Fermino Junior, Paulo Cesar Poeta; Universidade Federal do Acre; Silva, Ricardo Alexandre; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Scherwinski-Pereira, Jonny Everson; Embrapa Recursos Genéticos e Biotecnologia. |
| The objective of this study was to morpho-anatomically characterize nodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 µM) and BAP (0, 5, 10 µM). After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 µM). After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 µM), where the presence of multi-granular structures were observed.... |
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Palavras-chave: Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos 5.00.00.00-4 Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos Ciências Agrárias. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3248 |
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| Simão, Mariela Justiniano; Faria, Daniele Vidal; Werner, Elias Terra; Soares, Taís Cristina Bastos; Gontijo, Andreia Barcelos Passos Lima. |
| Bromeliads are an important group for the maintenance of the Atlantic Forest, with many threatened species due to exacerbated extraction and destruction of their natural habitats. Considering the need of developing protocols for the conservation of these species, the aim of this work was to evaluate the effect of different growth regulators in the in vitro induction of shoots of Billbergia euphemiae. Leaf explants were excised from seedlings derived from in vitro germination and grown on MS medium supplemented with NAA (0, 1 or 2 μM) and BA (0, 2, 4 or 6 μM) combinations. The evaluation of the number of shoots per explant, shoot length, number of leaves per shoot and longest leaf length average was carried out after 30 and 60 days of culture. The best in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.06.00-8 BA; Bromeliad; Micropropagation; NAA; Organogenesis Botânica Aplicada. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31694 |
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| Pavei, Ana Flávia; Fraga, Hugo Pacheco de Freitas; Vieira, Leila do Nascimento; Guerra, Miguel Pedro. |
| The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.00.00-0 Botânica in vitro culture; Micropropagation; Somatic embryo conversion; Feijoa. 2.03.06.00-8 Botânica Aplicada. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257 |
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| Haygert-Lencina,Kelen; Antônio-Bisognin,Dilson; Kielse,Paula; Pimentel,Nathalia. |
| Abtract Apuleia leiocarpa is an endangered tree species with high quality timber. Propagation by seed is difficult due to irregular fruiting and water-impermeable tegument, so asexual propagation is a promising alternative. This study aimed to evaluate in vitro and ex vitro rooting of A. leiocarpa and acclimatization of micropropagated plantlets. The hypothesis was that type of explant, indolebutyric acid (IBA) concentration and substrate influence the induction of adventitious roots in apuleia. For in vitro rooting, nodal segments and micro-cuttings were grown in Wood Plant Medium (WPM) with 0, 4.9, 9.8, 14.7 and 19.6 μM IBA. Compositions of commercial substrate + vermiculite + coarse sand and commercial substrate + vermiculite, in equal proportions, were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apuleia leiocarpa; Indolebutyric acid; Micropropagation; Micro-cuttings; Substrate composition. |
| Ano: 2017 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952017000800909 |
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| MARTINS,ADALVAN D.; MARTINS,JOÃO PAULO R.; BATISTA,LUCAS A.; DIAS,GABRIELEN M.G.; ALMEIDA,MIRIELLE O.; PASQUAL,MOACIR; SANTOS,HELOÍSA O. DOS. |
| Abstract The use of silicon in Billbergia zebrina cultivation in vitro is an alternative for optimizing micropropagation of this important ornamental plant species. The objective of the present study was to evaluate the growth and anatomical and physiological alterations in Billbergia zebrina (Herbert) Lindley plants as a function of different sources and concentrations of silicon during in vitro cultivation and acclimatization. The experimental design was completely randomized, with a double factorial arrangement and an additional control treatment (2 x 3 + 1). The first factor was relative to calcium silicate and sodium silicate added to the Murashige & Skoog culture medium; the second factor was related to its concentrations, 0.5, 1.0, and 2.0 mg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliad; Micropropagation; Plant anatomy; Plant physiology; Silicon. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703449 |
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| Registros recuperados: 121 | |
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