SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Moreira,Adônis; Fageria,Nand Kumar.

Alfalfa is an important forage crop with high nutritive value, although highly susceptible to soil acidity. Liming is one of the most efficient and prevailing practices to correct soil acidity and improve alfalfa yield. The objective of this study was to evaluate response to liming of alfalfa grown in a greenhouse on a Typic Quartzipsamment soil. The treatments consisted of four lime rates (0, 3.8, 6.6 and 10.3 Mg ha-1) and two cuts. Alfalfa dry matter increased quadratically with increasing lime rates. In general, dry matter yield was maximized by a lime rate of 8.0 Mg ha-1. Except for the control, the dry matter nutrient contents in the treatments were adequate. The positive linear correlation between root and nodule dry matter with lime rates indicated...

Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Palavras-chave: Medicago sativa L.; Shoot dry matter; Nodulation; Soil acidity; Nutrient uptake.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400022

Chagas,Eduardo; Araújo,Adelson Paulo; Alves,Bruno José Rodrigues; Teixeira,Marcelo Grandi.

Seeds with a high concentration of P or Mo can improve the growth and N accumulation of the common bean (Phaseolus vulgaris L.), but the effect of enriched seeds on biological N2 fixation has not been established yet. This study aimed to evaluate the effect of seeds enriched with P and Mo on growth and biological N2 fixation of the common bean by the 15N isotope dilution technique. An experiment was carried out in pots in a 2 x 3 x 2 x 2 factorial design in randomized blocks with four replications, comprising two levels of soil applied P (0 and 80 mg kg-1), three N sources (without N, inoculated with rhizobia, and mineral N), two seed P concentrations (low and high), and two seed Mo concentrations (low and high). Non-nodulating bean and sorghum were used...

Rhizobial Inoculation and Molybdenum Fertilization in Peanut Crops Grown in a No Tillage System After 20 Years of Pasture

Palavras-chave: Molybdenum; Nodulation; Phaseolus vulgaris.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000400009

Crusciol,Carlos Alexandre Costa; Ferrari Neto,Jayme; Mui,Tsai Siu; Franzluebbers,Alan Joseph; Costa,Cláudio Hideo Martins da; Castro,Gustavo Spadotti Amaral; Ribeiro,Lívia Cristina; Costa,Nídia Raquel.

ABSTRACT: Peanut (Arachis hypogea) is an important legume grain consumed by humans and utilized for effective nutrient cycling in a diverse cropping system. Areas that have been cultivated with perennial pasture for decades may have nutritional deficiencies and lack a sufficient population of atmospheric nitrogen-fixing bacteria. Molybdenum is an essential micronutrient that is part of the enzyme nitrogenase contained within symbiotic Bradyrhizobium bacteria, which are responsible for fixing nitrogen in legumes. Our objective was to evaluate the effects of application of Mo at different rates and a rhizobial inoculant on peanut growth characteristics. The experiment was conducted in the 2009/2010 growing season in a no-tillage cropping system following...

Effect of fertilizer-N application and seed coating with rhizobial inoculants on soybean yield in eastern Paraguay

Palavras-chave: Arachis hypogaea; Urochloa brizantha; Symbiotic fixation; Nodulation; Nitrogenase activity.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832019000100500

Kubota,Aki; Hoshiba,Kent; Bordon,Jorge.

Nitrogen removal in soybean grains at harvest may exceed biological N2 fixation, particularly if grain yields are as high as typically achieved on "Terra Rossa" soils of Eastern Paraguay. Applying N fertilizer or coating seeds with rhizobial inoculants that enhance nodulation may represent a way of balancing the N budget. However, the effects of such treatments appear to be highly site-specific. The objective of this study was to examine the effects of N application (N) and rhizobial inoculation (I) on nodulation, N accumulation and soybean yields in Eastern Paraguay. Field experiments were conducted in two consecutive soybean seasons. Dry conditions in the first year delayed sowing and reduced plant number m-2 and pod number plant-1. Grain yields were...

Seeds with high molybdenum concentration improved growth and nitrogen acquisition of rhizobium-inoculated and nitrogen-fertilized common bean plants

Palavras-chave: Glycine max; Inoculum; Nitrogen; Nodulation; Rhizobium.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000400027

Almeida,Fernanda Fátima Delgado; Araújo,Adelson Paulo; Alves,Bruno José Rodrigues.

Seeds of common bean (Phaseolus vulgaris) with high molybdenum (Mo) concentration can supply Mo plant demands, but to date no studies have concomitantly evaluated the effects of Mo-enriched seeds on plants inoculated with rhizobia or treated with N fertilizer. This work evaluated the effects of seed Mo on growth and N acquisition of bean plants fertilized either by symbiotic N or mineral N, by measuring the activities of nitrogenase and nitrate reductase and the contribution of biological N2 fixation at different growth stages. Seeds enriched or not with Mo were sown with two N sources (inoculated with rhizobia or fertilized with N), in pots with 10 kg of soil. In experiment 1, an additional treatment consisted of Mo-enriched seeds with Mo applied to the...

Land use and vegetation cover on native symbionts and interactions with cowpea

Palavras-chave: Phaseolus vulgaris; Nodulation; Biological N2 fixation; 15N isotope dilution; Ontogeny.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000200008

AGRIAMBI

Rocha,Beatriz C. F.; Santos,Enésia O. da S.; Santos,José G. D.; Takako,Adriana K.; Castro,Fabio J..

ABSTRACT Arbuscular mycorrhizal fungi and rhizobia are important components of agroecosystems and they respond to human interference. The objective of this study was to investigate native communities of those microorganisms in soil collected under the native forest, four pastures (Brachiaria brizantha, Panicum maximum, Arachis pintoi and Stylosanthes guianensis) and a fallow soil after maize cultivation, in interaction with cowpea (Vigna unguculata). The cowpea grew in a greenhouse until flowering. They were randomly distributed depending on soil, in five replications. The lowest mycorrhizal fungi sporulation and mycorrhizal root colonization occurred under the Panicum and forest soil. In the soils under forest and Stylosanthes, the cowpea did not exhibit...

Water restriction and physiological traits in soybean genotypes contrasting for nitrogen fixation drought tolerance

Palavras-chave: Arbuscular mycorrhiza; Rhizobia; Legal Amazon; Nodulation.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000200116

Scientia Agricola

Cerezini,Paula; Fagotti,Dáfila dos Santos Lima; Pípolo,Antonio Eduardo; Hungria,Mariangela; Nogueira,Marco Antonio.

ABSTRACT Biological nitrogen fixation (BNF) is essential to the economic viability of the soybean [Glycine max (L.) Merrill] crop in Brazil, but drought may impair the BNF processes. We evaluated physiological traits of nitrogen fixation drought-tolerant (NFDT) (R01-581F, R01-416F and R02-1325) and drought-susceptible (CD 215 and BRS 317) genotypes of soybean subjected to drought or regular water supply between 45 and 55 days after emergence, in an experiment under greenhouse conditions in pots containing non-sterile soil. R01-581F had more stable photosynthetic and transpiration rates, and higher intercellular CO2 levels under drought. Drought reduced the chlorophyll concentration in all genotypes, but with less intensity in R01-581F and R02-1325. The...

Sesbania virgata stimulates the occurrence of its microsymbiont in soils but does not inhibit microsymbionts of other species

Palavras-chave: Glycine max; Abiotic stress; Nodulation; Total soluble sugars; Ureides.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200110

Scientia Agricola

Florentino,Ligiane Aparecida; Guimarães,Ana Paula; Rufini,Márcia; Silva,Krisle da; Moreira,Fátima Maria de Souza.

The legume species Sesbania virgata establishes a specific and efficient symbiosis with Azorhizobium doebereinerae. Previous studies have shown that A. doebereinerae occurrence correlates to the presence of S. virgata. This work aimed to evaluate the occurrence of A. doebereinerae and of other nitrogen-fixing Leguminosae-nodulating bacteria (NFLNB) in soil samples collected adjacent to and 10 m away from the stems of five S. virgata plants in pasture areas. Symbiotic characteristics of isolates from these NFLNB populations were also studied. S. virgata and the four promiscuous legume species Leucaena leucocephala, Macroptilium atropurpureum, Phaseolus vulgaris and Vigna unguiculata were inoculated with soil samples to trap A. doebereinerae and other NFLNB....

Caracterização de rizóbios (Bradyrhizobium japonicum) e produtividade da soja

Palavras-chave: Azorhizobium; Cupriavidus; Leguminosae; Nodulation; Nitrogen-fixing species.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500012

Scientia Agricola

LIMA,S.C.; LOPES,E.S.; LEMOS,E.G.M..

Fez-se um estudo de campo sobre a competição e a dinâmica de ocupação de sítios de nodulação de estirpes de Bradyrhizobium japonicum naturalizadas ou inoculadas em sementes. O ensaio foi instalado em Latossolo Vermelho Escuro utilizando blocos ao acaso, com 8 repetições dos seguintes tratamentos: A) controle sem inoculante; B) controle sem inoculante, com adubação nitrogenada; C) inoculante com cerca de 107 rizóbios/g (8g/kg de semente); D) inoculação com cerca de 1010 rizóbios/g (8g/kg de semente). Os inoculantes foram preparados com as estirpes recomendadas na ocasião, SMS - 314 (= SEMIA 587) e SMS - 463 (= SEMIA 5019 = 29W). A identificação das estirpes formadoras dos nódulos foi realizada em amostras coletadas aos 10, 42 e 70 dias após a germinação....

Production and disease resistance of elite black bean lines previously selected using mineral nitrogen fertilization cultivated with natural versus artificial nitrogen supplementation.

Palavras-chave: Soja; Nodulação; Bactéria; Nodulation.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000300003

So many rhizobial partners, so little nitrogen fixed: The intriguing symbiotic promiscuity of common bean (Phaseolus vulgaris L.).

DIAS, P. A. S.; MELO, P. G. S.; MELO, L. C.; SOUZA, T. L. P. O.; FARIA, L. C. de; FERREIRA, E. P. B.; PEREIRA, H. S..

Nitrogen (N) is the most important soil nutrient for common beans; the main sources are mineral N fertilizers and symbiotic N fixation (SNF). The common bean (Phaseolus vulgaris) is a N fixing species, but breeding programs have neglected this fact. We investigates black seeded elite lines grown with mineral N fertilization or using inoculation with rhizobia to select lines with broad adaptability, high yield stability, and high agronomic performance. Fifteen black seeded common bean elite lines and cultivars, all of them selected under mineral N fertilization, were evaluated in major bean crop regions in the Brazilian states of Goiás, Paraná and Distrito Federal in different growing seasons and years, totaling 13 environments (combination of...

Tipo: Artigo de periódico

Palavras-chave: Symbiotic N fixation; Feijão; Phaseolus Vulgaris; Nodulação; Rhizobium; Fixação de Nitrogênio; Melhoramento Genético Vegetal; Beans; Plant breeding; Nitrogen fixation; Nodulation.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122030

Rhizobial characterization in revegetated areas after bauxite mining.

MOURA, F. T.; RIBEIRO, R. A.; HELENE, L. C. F.; NOGUEIRA, M. A.; HUNGRIA, M..

Tipo: Artigo de periódico

Palavras-chave: Fixação de Nitrogênio; Nodulação; Feijão; Beans; Nitrogen fixation; Nodulation.

Ano: 2022

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150683

Compatibilidade do tratamento de sementes de feijão-caupi com fungicidas e inoculação com estirpes de Bradyrhizobium.

BORGES, W. L.; PRIN, Y.; DUCOUSSO, M.; LE ROUX, C.; FARIA, S. M. de.

Little is known regarding how the increased diversity of nitrogen-fixing bacteria contributesto the productivity and diversity of plants in complex communities. However, some authorshave shown that the presence of a diverse group of nodulating bacteria is required for dif-ferent plant species to coexist. A better understanding of the plant symbiotic organismdiversity role in natural ecosystems can be extremely useful to define recovery strategies ofenvironments that were degraded by human activities. This study used ARDRA, BOX-PCRfingerprinting and sequencing of the 16S rDNA gene to assess the diversity of root nodulenitrogen-fixing bacteria in former bauxite mining areas that were replanted in 1981, 1985,1993, 1998, 2004 and 2006 and in a native forest....

Tipo: Artigo de periódico

Palavras-chave: Symbiotic nitrogen fixation.; Fixação simbiótica de nitrogênio; Nodulação; Simbiose; Nodulation; Symbiosis..

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1043239

Capacidade de genótipos de feijoeiro de fixar N2 atmosférico.

SILVA NETO, M. L. da; SMIDERLE, O. J.; SILVA, K. da; FERNANDES JUNIOR, P. I.; XAVIER, G. R.; ZILLI, J. E..

O objetivo deste trabalho foi avaliar a compatibilidade do tratamento de sementes com fungicidas e a inoculação com estirpes de Bradyrhizobium em feijão-caupi. Em laboratório, avaliou-se a sobrevivência de células nas sementes da cultivar BRS Guariba, tratadas ou não com fungicidas (fludioxonil, carbendazim, carbendazim + thiram e carboxin + thiram) e inoculadas ou não com Bradyrhizobium (estirpes UFLA3‑84, BR 3267, INPA3‑11B e BR 3262). Em casa de vegetação, conduziu-se experimento em vasos de Leonard, com os mesmos tratamentos. Foram avaliados: massa de matéria seca da parte aérea, além de número e massa de nódulos 25 dias após a emergência das plantas. No campo, dois experimentos foram conduzidos, tendo-se utilizado a estirpe BR...

Tipo: Artigo de periódico

Palavras-chave: Fixação biológica de nitrogênio; Biological nitrogen fixation.; Feijão; Doença; Semente; Nodulação; Vigna Unguiculata.; Nodulation; Bradyrhizobium..

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/953437

Efeito de doses de inoculante turfoso na fixacao biologica do nitrogenio pela cultura da soja.

PEREIRA, P. A. A.; ARAUJO, R. S.; ROCHA, R. E. M. D.; STEINMETZ, S..

Foi conduzido em campo, programa de tiragem para avaliar a potencialidade de diversos genótipos de feijoeiro (Phaseolus vulgaris L.) para fixação de N2 atmosférico. Observou-se uma ampla variabilidade no peso da matéria seca dos nódulos e na atividade da ntrogenase (N2-ase) dos genótipos estudados. A atividade media da nitrogenase (N2-ase) foi maior na floração do que no enchimento dos grãos das plantas de feijão, o que indicou uma senescência dos nódulos nesta fase fisiológica. Mais de 40% dos genótipos estudados apresentaram uma massa nodular superior a 92,5 mg de peso da matéria seca dos nódulos/planta, e alguns alcançaram 200 mg deste peso, podendo ser comparados a massa nodular de outras leguminosas tidas como eficientes na simbiose com bactérias do...

Tipo: Artigo de periódico

Palavras-chave: Cerrado; Feijão; Genótipo; Nitrogênio; Nodulação; Phaseolus Vulgaris; Fixação de Nitrogênio; Beans; Nodulation; Nitrogen fixation.

Ano: 1984

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/205104

BRANDAO JUNIOR, O.; HUNGRIA, M..

RESUMO: A inoculação e a reinoculação da soja [Glycine max (L.) Merrill] são essenciais para garantir a maximização do processo de fixação biológica do N2. A maioria dos inoculantes comercializados para a soja no País utiliza, como veículo, a turfa, mas existem controvérsias sobre a dose a ser recomendada, especialmente em condições desfavoráveis ou em áreas já inoculadas. Assim, para a região central do Brasil, tem sido recomendado usar 1.000 g de inoculante/50 kg de sementes, com solução açucarada como adesivo. Neste estudo, porém, constatou-se que a dose máxima de turfa aderida às sementes está entre 500 g e 600 g de inoculante/50 kg. O uso de doses superiores resultará, portanto, em acúmulo na semeadora, dificultando o plantio. Em condições de casa de...

Tipo: Separatas

Palavras-chave: Inoculation.; Inoculação; Nodulação; Semente; Tratamento; Soja.; Bradyrhizobium; Nodulation; Seed treatment..

Ano: 2000

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/444842

Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR.

Horizontal transfer of symbiosis genes within and between rhizobial genera: occurrence and importance.

BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO, F. C.; HUNGRIA, M..

Resumo O objetivo deste trabalho foi avaliar a expressão, por RT-qPCR, dos genes de nodulação nodC e nodW e do gene nopP da estirpe CPAC 15, que provavelmente atuam na infecção das raízes da soja. Foram realizados dois experimentos. No primeiro, a expressão dos genes foi avaliada nas células após a incubação com genisteína por 15 min, 1, 4 e 8 horas. Os resultados revelaram que os três genes apresentaram maior expressão imediatamente após o contato com o indutor (15 min). No segundo experimento, a bactéria foi cultivada na presença de indutores (genisteína ou exsudatos de sementes de soja) por 48 horas. A expressão dos três genes foi maior na presença de genisteína, com valores de expressão para nodC, nodW e nopP superiores ao controle. Os resultados...

Tipo: Artigo de periódico

Palavras-chave: Genes de nodulação; Sistema de secreção tipo III; Nodulation genes; Fixação de nirogênio.; Soja; Flavonóide; Nodulação.; Soybeans; Flavonoids; Gene expression; Nitrogen fixation; Nodulation; Type III secretion system..

Ano: 2009

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/631360