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| Registros recuperados: 40 | |
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| Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.. |
| An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to... |
| Tipo: Text |
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf |
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| Butot, Sophie; Le Guyader, Soizick; Krol, Joanna; Putallaz, Thierry; Amoroso, Richard; Sanchez, Gloria. |
| Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 NoV samples, including different subtypes of NoV genogroup I and II. The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized kits evaluated failed to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3,... |
| Tipo: Text |
Palavras-chave: Norovirus; Real-time RT-PCR; Commercial kit. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11760/8473.pdf |
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| Zidane, Mohamed; Le Guyader, Soizick; Pommepuy, Monique. |
| During the winter 2000/2001, several outbreaks associated with the consumption of shellfish were declared in France. Following these events, a close cooperation was organized between administrations and laboratories (DGAL, DPMA, InVS, AFSSA, Ifremer, CHU-Dijon and CNC) to gather competences and to manage the situation. This report synthesizes the information collected during these outbreaks. From the outset, the epidemiologic investigations directed the research towards viral contamination and shellfish as contamination source. Several outbreaks were investigated. Oysters and stools samples from ill patients were analysed by RT-PCR for viruses (enterovirus, rotavirus, norovirus and astrovirus). The genic amplification was done by using the same primers in... |
| Tipo: Text |
Palavras-chave: Épidémie; Gastro-entérites; Huîtres; Contamination virale; RT-PCR; Norovirus; TIAC. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00068/17941/15476.pdf |
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| Le Guyader, Soizick; Garry, Pascal; Ollivier, Joanna; Piquet, Jean-come. |
| The shellfish safety has long been considered a public health problem. The implementation of a regulation based on a bacterial indicator of faecal contamination has significantly reduced outbreaks of bacterial origin. However, epidemics of viral gastroenteritis due to the presence of noroviruses from human sewage persist. We demonstrated few years ago that oysters are not just a passive filter but are able to select some viral strains, via the presence of specific ligands, very similar to ligands observed in humans for these same virus. Ongoing developments, in conjunction with the application of metagenomic tools, will allow us to better understand the fate of some human pathogens after their release into the coastal environment. |
| Tipo: Text |
Palavras-chave: Shellfish; Microbiology; Oysters; Norovirus. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71141/69457.pdf |
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| A review of the biology, epidemiology, diagnosis and public health importance of foodborne viruses was performed. Data needs to support a risk assessment were also identified. In addition possible control options and their anticipated impact to prevent or reduce the number of foodborne viral human infections were identified, including the scientific reasons for and against the establishment of food safety criteria and process hygiene criteria for viruses for certain food categories. Food may be contaminated by virus during all stages of the food supply chain, and transmission can occur by consumption of food contaminated during the production process (primary production, or during further processing), or contaminated by infected food handlers. Transmission... |
| Tipo: Text |
Palavras-chave: Food borne viruses; Norovirus; Hepatitis; Microbiological criteria; Molluscs; Fresh produce. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00066/17734/15255.pdf |
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| Strubbia, Sofia; Schaeffer, Julien; Oude Munnink, Bas B.; Besnard, Alban; Phan, My V. T.; Nieuwenhuijse, David F.; De Graaf, Miranda; Schapendonk, Claudia M. E.; Wacrenier, Candice; Cotten, Matthew; Koopmans, Marion P. G.; Le Guyader, Soizick. |
| Metagenomic sequencing is a promising method to determine the virus diversity in environmental samples such as sewage or shellfish. However, to identify the short RNA genomes of human enteric viruses among the large diversity of nucleic acids present in such complex matrices, method optimization is still needed. This work presents methodological developments focused on norovirus, a small ssRNA non-enveloped virus known as the major cause of human gastroenteritis worldwide and frequently present in human excreta and sewage. Different elution protocols were applied and Illumina MiSeq technology were used to study norovirus diversity. A double approach, agnostic deep sequencing and a capture-based approach (VirCapSeq-VERT) was used to identify norovirus in... |
| Tipo: Text |
Palavras-chave: Norovirus; Sewage; Oysters; Metagenomic sequencing; Metavirome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00586/69765/67656.pdf |
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| Desdouits, Marion; Wacrenier, Candice; Ollivier, Joanna; Schaeffer, Julien; Le Guyader, Soizick. |
| Human noroviruses (NoV) cause epidemics of acute gastroenteritis (AGE) worldwide and can be transmitted through consumption of contaminated foods. Fresh products such as shellfish can be contaminated by human sewage during production, which results in the presence of multiple virus strains, at very low concentrations. Here, we tested a targeted metagenomics approach by deep-sequencing PCR amplicons of the capsid (VP1) and polymerase (RdRp) viral genes, on a set of artificial samples and on shellfish samples associated to AGE outbreaks, to evaluate its advantages and limitations in the identification of strains from the NoV genogroup (G) II. Using artificial samples, the method allowed the sequencing of most strains, but not all, and displayed variability... |
| Tipo: Text |
Palavras-chave: Norovirus; Foodborne virus; Metagenomics; Amplicon deep sequencing; Viral diversity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00646/75831/76818.pdf |
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| Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Cochennec-laureau, Nathalie; Le Guyader, Soizick. |
| Les norovirus (NoV) sont responsables des épidémies hivernales de gastroentérites aiguës dans la population. Ils sont excrétés dans les fèces humains, puis se retrouvent, après épuration insuffisante, dans le milieu marin naturel où ils peuvent contaminer des coquillages, qui à leur tour risquent de déclencher des gastroentérites chez les consommateurs. Cette étude vise à améliorer les connaissances sur la contamination des coquillages en élevage en rivière d’Auray par les NoV, en lien avec les bactéries témoins de contamination fécale Escherichia coli (E. coli). L’objectif est de suivre les flux de NoV et d’E. coli apportés par différents rejets (dont une station d’épuration) impactant la zone conchylicole et leur influence sur la qualité des coquillages... |
| Tipo: Text |
Palavras-chave: Norovirus; Coquillages; Contamination; Station d’épuration; Flux; Rivière d’Auray; Escherichia coli.. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00267/37778/35829.pdf |
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| Le Guyader, Soizick; Maalouf, Haifa; Le Pendu, Jacques. |
| Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of genogroup I strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to... |
| Tipo: Text |
Palavras-chave: Huîtres; Norovirus; Ligand; Sélection de souches; Oysters; Norovirus; Ligand; Strain selection. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26357/24514.pdf |
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| Strubbia, Sofia; Phan, My V. T.; Schaeffer, Julien; Koopmans, Marion; Cotten, Matthew; Le Guyader, Soizick. |
| This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The third method was based on a non-specific biophysical precipitation with polyethylene glycol. Full genomes of a number of common human enteric viruses including norovirus, rotavirus, husavirus, enterovirus and astrovirus were obtained. In stool samples full norovirus genome were detected as well as partial enterovirus genome. A variety of norovirus sequences was... |
| Tipo: Text |
Palavras-chave: Human enteric viruses; Norovirus; Sewage; Metagenomic; Virome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00512/62332/66592.pdf |
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| Benabbes, Laila; Ollivier, Joanna; Schaeffer, Julien; Parnaudeau, Sylvain; Rhaissi, Houria; Nourlil, Jalal; Le Guyader, Soizick. |
| The aim of this study was to evaluate the presence of human enteric viruses in shellfish collected along the Mediterranean Sea and Atlantic Coast of Morocco. A total of 77 samples were collected from areas potentially contaminated by human sewage. Noroviruses were detected in 30 % of samples, with an equal representation of GI and GII strains, but were much more frequently found in cockles or clams than in oysters. The method used, including extraction efficiency controls, allowed the quantification of virus concentration. As in previous reports, results showed levels of contamination between 100 and 1,000 copies/g of digestive tissues. Sapoviruses were detected in 13 % of samples mainly in oyster and clam samples. Hepatitis A virus was detected in two... |
| Tipo: Text |
Palavras-chave: Shellfish; Norovirus; Hepatitis A virus; Environmental conditions; Quantification; Morocco. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00130/24102/22489.pdf |
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| Luz Vilarino, M.; Le Guyader, Soizick; Polo, David; Schaeffer, Julien; Krol, Joanna; Romalde, Jesus L.. |
| Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated... |
| Tipo: Text |
Palavras-chave: Seafood industry; Viral quantification; Viral prevalence; Norovirus; Hepatitis A virus; Enteric viruses; Molluscs. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6957.pdf |
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| Lowther, J. A.; Bosch, A.; Butot, S.; Ollivier, Joanna; Maede, D.; Rutjes, S. A.; Hardouin, G.; Lombard, B.; In'T Veld, P.; Leclercq, A.. |
| Hepatitis A virus (HAV) and norovirus are important agents of food-borne human viral illness, with common vehicles including bivalve molluscan shellfish, soft fruit and various vegetables. Outbreaks of viral illness due to contamination of the surfaces of foods, or food preparation surfaces by for example infected food handlers are also common. Virus analysis of food matrices can contribute towards risk management for these hazards and a two-part technical specification for determination of Hepatitis A virus and norovirus in food matrices (ISO/TS 15216:2013) was published jointly by the European Committee for Standardisation and the International Organization for Standardization in 2013. As part of the European Mandate No. M381 to validate 15 standards in... |
| Tipo: Text |
Palavras-chave: Norovirus; Hepatitis A virus; Real-time RT-PCR; Validation; Standardization; Bivalve molluscan shellfish; Soft fruit; Vegetables; Bottled water; Surfaces. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00476/58776/61442.pdf |
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| Desdouits, Marion; De Graaf, Miranda; Strubbia, Sofia; Oude Munnink, Bas B.; Kroneman, Annelies; Le Guyader, Soizick; Koopmans, Marion P. G.. |
| Foodborne viral infections rank among the top 5 causes of disease, with noroviruses and hepatitis A causing the greatest burden globally. Contamination of foods by infected food handlers or through environmental pollution are the main sources of foodborne illness, with a lesser role for consumption of products from infected animals. Viral partial genomic sequencing has been used for more than two decades to track foodborne outbreaks and whole genome or metagenomics next-generation-sequencing (NGS) are new additions to the toolbox of food microbiology laboratories. We discuss developments in the field of targeted and metagenomic NGS, with an emphasis on application in food virology, the challenges and possible solutions towards future routine application |
| Tipo: Text |
Palavras-chave: Next-generation sequencing; Metagenomics; Foodborne virus; Food virology; Human enteric virus; Norovirus. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00641/75330/75988.pdf |
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| Registros recuperados: 40 | |
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