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| Registros recuperados: 49 | |
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| Olabuenaga,Susana E.. |
| The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well... |
| Tipo: Journal article |
Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010 |
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| Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis. |
| Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf |
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| Gonzalez-aravena, Marcelo; Perez-troncoso, Carolina; Urtubia, Rocio; Branco, Paola; Machado Cunha Da Silva, Jose Roberto; Mercado, Luis; De Lorgeril, Julien; Bethke, Jorn; Paschke, Kurt. |
| In the Antarctic marine environment, the water temperature is usually between 2 and - 1.9 degrees C. Consequently, some Antarctic species have lost the capacity to adapt to sudden changes in temperature. The study of the immune response in Antarctic sea urchin (Sterechinus neumayeri) could help us understand the future impacts of global warming on endemic animals in the Antarctic Peninsula. In this study, the Antarctic sea urchins were challenged with lipopolysaccharides and Vibrio alginolitycus. The cellular response was evaluated by the number of coelomocytes and phagocytosis. A significant increase was observed in red sphere cells and total coelomocytes in animals exposed to LPS. A significant rise in phagocytosis in animals stimulated by LPS was also... |
| Tipo: Text |
Palavras-chave: Sea urchin; Antarctica; Sterechinus neumayeri; Coelomocytes; Phagocytosis; Gene expression. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00439/55012/56441.pdf |
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| Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco. |
| In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides... |
| Tipo: Text |
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf |
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| Auffret, Mathieu; Diouris, M; Herry, A. |
| Bivalve molluscs possess several types of hemolymph cells (hemocytes), involved in internal defense and other physiological functions. Studies in pathological and experimental conditions have shown their micro- and macrophagic ability. However, the mechanisms of intracellular digestion are incompletely known in these organisms. The lysosomal apparatus of hemocytes was therefore investigated in several species by hydrolase localization with the electron microscope. The cytoplasmic granules of granulocytes displayed a strong acid beta -glycerophosphatase activity. Previous observations had shown that these granules may contain phagocytized material. This indicates that intracellular digestion is achieved in these organelles. Acid beta -glycerophosphatase... |
| Tipo: Text |
Palavras-chave: Bivalvia; Phagocytosis; Cells; Ultrastructure; Haemolymph. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1631.pdf |
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| Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio. |
| Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland.... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf |
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| Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan. |
| Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf |
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| Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. |
| To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... |
| Tipo: Text |
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf |
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| Delaporte, Maryse; Soudant, P; Lambert, C; Moal, Jeanne; Pouvreau, Stephane; Samain, Jean-francois. |
| The aim of this study was to test the effect of food quantity on energy storage and defense capacities of oysters during a reproductive cycle. One-year-old Crassostrea gigas oysters were fed two different dietary rations (4% and 12% of oyster dry weight in algal dry weight per day) in controlled experimental conditions over an annual cycle. Oyster dry weights, carbohydrate and lipid contents, energetic adenylate charge, and hemocyte parameters of oysters were significantly affected by reproductive processes related to seasonal temperature variation and, to a lesser extent, by the dietary rations. Energy parameters decreased during gametogenesis as gonads developed then increased during the gonad resorption phase. The additional energy provided to oysters... |
| Tipo: Text |
Palavras-chave: Reactive oxygen species production; Reproduction; Phagocytosis; Hemocyte parameters; Energy storage; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1549.pdf |
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| Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie. |
| The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days.... |
| Tipo: Text |
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf |
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| Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine. |
| Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf |
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| Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry. |
| Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and... |
| Tipo: Text |
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf |
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| Bettencourt, R; Dando, P; Rosa, D; Riou, V; Colaco, A; Sarrazin, Jozee; Sarradin, Pierre-marie; Santos, R. |
| The deep-sea hydrothermal vent mussel Bathymodiolus azoricus has been the subject of several studies aimed at understanding the physiological adaptations that vent animals have developed in order to cope with the particular physical and chemical conditions of hydrothermal environments. In spite of reports describing successful procedures to maintain vent mussels under laboratory conditions at atmospheric pressure, few studies have described the mussel's physiological state after a long period in aquaria. In the present study, we investigate changes in mucocytes and hemocytes in B. azoricus over the course of several months after deep-sea retrieval. The visualization of granules of mucopolysaccharide or glycoprotein was made possible through their inherent... |
| Tipo: Text |
Palavras-chave: Vent; Hydrothermal vent; Bathymodiolus azoricus; Aquarium acclimatization; Phagocytosis; Alcian blue Periodic Acid Schiff staining; Mucopolysaccharide. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4357.pdf |
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| Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan. |
| Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster... |
| Tipo: Text |
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf |
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| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. |
| The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf |
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| Mutua,P. M; Gicheru,M. M; Makanya,A. N; Kiama,S. G. |
| In mammals, surface respiratory macrophages (SM) are known to play a foremost role in protecting the respiratory system by providing first line of defense through engulfing pathogens and particulate matter respired with air. It has been reported that the pulmonary cellular defense system of domestic birds is inadequate. In particular, low number of SM and even lack of the cells in a healthy avian respiratory system have been associated with susceptibility of domestic birds to respiratory diseases. In an endeavor to resolve the existing controversy, the quantitative and qualitative attributes of the surface respiratory macrophages of the domestic duck and of the domestic rabbit were compared under similar experimental conditions. Quantitatively, the rabbit... |
| Tipo: Journal article |
Palavras-chave: Avian; Cellular defense; Lung; Phagocytosis; Rabbit; Surface macrophages. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200008 |
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| Registros recuperados: 49 | |
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