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Registros recuperados: 43
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Demographic history of wood stork (Mycteria americana) Brazilian Pantanal colonies revealed by mitochondrial DNA analysis Genet. Mol. Biol.
Lopes,Iara Freitas; Brito,Reinaldo Alves de; Henrique-Silva,Flávio; Del Lama,Silvia Nassif.
We used mitochondrial DNA (mtDNA) sequences to investigate the demographic history of the wood stork (Mycteria americana) populations in the Brazilian Pantanal. Sequences of 390/460 bp fragment of the mtDNA control region were analyzed in 62 wood stork specimens from 8 colonies using neutrality tests, phylogeographic, and coalescent analyses. Population expansion was supported by the significantly negative values of Tajima’s (D = -2.071) and Fu’s (Fs = -14.544) statistics and the unimodal pattern of mismatch distribution. Nested clade analyses indicated a historic range expansion event and recurrent gene flow that was restricted by isolation by distance as explanations for the haplotype distribution among the sampled colonies. High genetic diversity and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wood stork; Mycteria americana; Pantanal; Mitochondrial DNA; Phylogeography.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200008
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Molecular evidence for a recent demographic expansion in the puma (Puma concolor) (Mammalia, Felidae) Genet. Mol. Biol.
Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo.
The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018
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Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA Genet. Mol. Biol.
Freire,Maíra C. M.; Oliveira,Luiz O. de; Almeida,Álvaro M. R. de; Schuster,Ivan; Moreira,Maurilio A.; Liebenberg,Merion M.; Mienie,Charlotte M. S..
Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences...
Palavras-chave: Phakopsora pachyrhizi; Diversity; Internal transcribed spacer; Asian soybean rust; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020
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Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic Genet. Mol. Biol.
Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p < 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p < 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026
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Phylogeography and RAPD-PCR variation in Hoplias malabaricus (Bloch, 1794) (Pisces, Teleostei) in southeastern Brazil Genet. Mol. Biol.
Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio.
In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Nei’s genetic distance) suggest the existence of two genetically different groups, one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005
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Mitochondrial DNA phylogeography of Brazilian populations of Drosophila buzzatii Genet. Mol. Biol.
Brito,Reinaldo A. de; Manfrin,Maura H.; Sene,Fabio M..
The Argentinean Chaco region has been considered the center of origin of Drosophila buzzatii in South America because it contains most of the chromosomal polymorphism detected in natural populations. Two hypotheses have been put forward to explain the distribution of D. buzzatii in Brazil, one proposing that it has only recently passively colonized Brazil via human dispersal and the other suggesting that D. buzzatii has actively migrated to Brazil some time ago. Data from chromosomal inversions support recent colonization, whereas data from allozymes and mtDNA variation indicate that D. buzzatii has been in Brazil longer, favoring an active dispersal hypothesis. In our present work we analyzed data on 56 South American flies, mostly from Brazil, sequenced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila buzzatii; Population structure; Mitochondrial DNA; Phylogeography; Brazil; Climatic cycles.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200009
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Population structure of the Monocelis lineata (Proseriata, Monocelididae) species complex assessed by phylogenetic analysis of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene Genet. Mol. Biol.
Sanna,Daria; Lai,Tiziana; Francalacci,Paolo; Curini-Galletti,Marco; Casu,Marco.
Monocelis lineata consists of a complex of sibling species, widespread in the Mediterranean and Atlantic Ocean. Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) of M. lineata, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence of at least two Mediterranean sibling species. Thus validated, the method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microturbellaria; Sequencing; Sibling species; Phylogeography; Phylogeny.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400031
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Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor Genet. Mol. Biol.
Colombi,Victor Hugo; Lopes,Silvia Ramira; Fagundes,Valéria.
Akodon cursor occurs in dense rainforest from northern (8º S) to southern (26º S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15º S) and the other southern (19-26º S) populations. The center of geographic distribution (15-20º S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19º S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Akodontini; Atlantic Forest; Cytochrome b; Gene flow; Phylogeography; Rodentia.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400029
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The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) Genet. Mol. Biol.
Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B..
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018
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Population genetic analysis of Caiman crocodilus (Linnaeus, 1758) from South America Genet. Mol. Biol.
Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P..
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006
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Phylogenetic relationships among Capuchin (Cebidae, Platyrrhini) lineages: An old event of sympatry explains the current distribution of Cebus and Sapajus Genet. Mol. Biol.
Martins-Junior,Antonio Marcio Gomes; Carneiro,Jeferson; Sampaio,Iracilda; Ferrari,Stephen F.; Schneider,Horacio.
Abstract Capuchin monkeys are currently represented by four species of Cebus and eight of Sapajus. This group is taxonomically complex and several questions still need to be clarified. In the current study, using mtDNA markers and a larger sample representation than in previous studies, we seek to understand the phylogenetic relationships among the capuchin lineages and their historical biogeography. All 12 species of capuchins were analyzed for the mitochondrial Control Region and Cytochrome b to test two biogeographical hypotheses: “Reinvasion of the Amazon (ROA)” and “Sympatric Evolution (SEV)”. The phylogenetic relationships among distinct lineages within genera is consistent with an evolutionary diversification pattern probably resulting from an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capuchins; Phylogeography; Phylogeny; Taxonomy; Biogeography.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400699
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Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history Genet. Mol. Biol.
da Silva Santos,Anelisie; Trigo,Tatiane Campos; de Oliveira,Tadeu Gomes; Silveira,Leandro; Eizirik,Eduardo.
Abstract The pampas cat is a small felid that occurs in open habitats throughout much of South America. Previous studies have revealed intriguing patterns of morphological differentiation and genetic structure among its populations, as well as molecular evidence for hybridization with the closely related L. tigrinus. Here we report phylogeographic analyses encompassing most of its distribution (focusing particularly on Brazilian specimens, which had been poorly sampled in previous studies), using a novel dataset comprising 2,143 bp of the mitogenome, along with previously reported mtDNA sequences. Our data revealed strong population strutucture and supported a west-to-east colonization process in this species’ history. We detected two population expansion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogeography; Population genetics; Mitochondrial DNA; Conservation genetics; Historical demography.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200273
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Genetic variability in mitochondrial and nuclear genes of Larus dominicanus (Charadriiformes, Laridae) from the Brazilian coast Genet. Mol. Biol.
Dantas,Gisele Pires de Mendonça; Meyer,Diogo; Godinho,Raquel; Ferrand,Nuno; Morgante,João Stenghel.
Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is a common species with a wide distribution in the Southern Hemisphere. In this study, we used mitochondrial and nuclear markers to examine the genetic variability of Kelp Gull populations on the Brazilian coast and compared this variability with that of sub-Antarctic island populations of this species. Kelp Gulls showed extremely low genetic variability for mitochondrial markers (cytb and ATPase) and high diversity for a nuclear locus (intron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytb; FIB7 intron; Phylogeography; Recent origin; Selective sweep.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500023
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Genetic structure of the snakehead murrel, Channa striata (channidae) based on the cytochrome c oxidase subunit I gene: influence of historical and geomorphological factors Genet. Mol. Biol.
Jamaluddin,Jamsari Amirul Firdaus; Pau,Tan Min; Siti-Azizah,Mohd Nor.
Nucleotide sequences of a partial cytochrome c oxidase subunit I gene were used to assess the manner in which historical processes and geomorphological effects may have influenced genetic structuring and phylogeographic patterns in Channa striata. Assaying was based on individuals from twelve populations in four river systems, which were separated into two regions, the eastern and western, of the biodiversely rich state of Perak in central Peninsular Malaysia. In 238 specimens, a total of 368-bp sequences with ten polymorphic sites and eleven unique haplotypes were detected. Data on all the twelve populations revealed incomplete divergence due to past historical coalescence and the short period of separation. Nevertheless, SAMOVA and F ST revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; MtDNA COI; Phylogeography; Population structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100026
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Molecular phylogeny and classification of the chemosymbiotic bivalve family Lucinidae (Mollusca: Bivalvia) ArchiMer
Taylor, John D.; Glover, Emily A.; Smith, Lisa; Dyal, Patricia; Williams, Suzanne T..
A new molecular phylogeny of the chemosymbiotic bivalve family Lucinidae is presented. Using sequences from the nuclear 18S and 28S rRNA genes and the mitochondrial gene cytochrome b, 105 specimens were analysed representing 87 separate species classified into 47 genera. Samples were collected from a wide range of habitats including mangroves, seagrass beds, shallow sands, offshore muds, and hydrocarbon seeps at depths ranging from the intertidal to over 2000 m. A chronogram, derived from the combined molecular tree, was calibrated using ten lucinid fossils. The trees show five well-supported clades and two single branches of Fimbria fimbriata (Linnaeus, 1758) and Monitilora ramsayi (Smith, 1885). A new classification of Lucinidae is proposed with seven...
Tipo: Text Palavras-chave: Chemosymbiosis; Classification; Fossil record; Habitats; Phylogeography.
Ano: 2011 URL: https://archimer.ifremer.fr/doc/00467/57833/60131.pdf
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Population structure enhances perspectives on regional management of the western Indian Ocean green turtle ArchiMer
Bourjea, Jerome; Mortimer, Jeanne A.; Garnier, Julie; Okemwa, Gladys; Godley, Brendan J.; Hughes, George; Dalleau, Mayeul; Jean, Claire; Ciccione, Stephane; Muths, Delphine.
To refine our understanding of the spatial structure of the green turtle (Chelonia mydas) populations in the South West Indian Ocean (SWIO), we analysed patterns of mitochondrial DNA (396 base pairs control region fragment) variation among 171 samples collected at five distinct locations (Kenya, Northern Mozambique, and three locations in the Republic of Seychelles: the Granitic, Amirantes, and Farquhar groups) and compared them to genetic data (n = 288), previously collected from 10 southern locations in the SWIO. We also analysed post-nesting satellite tracks (n = 4) from green turtles nesting in the Amirantes group. Pairwise comparisons of haplotype frequencies showed significant genetic differentiation amongst rookeries and suggest that the SWIO hosts...
Tipo: Text Palavras-chave: Indian Ocean; MtDNA; Satellite tracking; Phylogeography; Management unit; Chelonia mydas.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00266/37732/36062.pdf
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Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses ArchiMer
Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf
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Molecular evolution and phylogeography of infectious hematopoietic necrosis virus with a focus on its presence in France over the last 30 years ArchiMer
Bellec, Laure; Louboutin, Lenaig; Cabon, Joelle; Castric, Jeanne; Cozien, Joelle; Thiery, Richard; Morin, Thierry.
Infectious hematopoietic necrosis virus (IHNV) is among the most important pathogens affecting the salmonid industry. Here, we investigated the molecular evolution and circulation of isolates from 11 countries or regions all over the world, with a special focus on the epidemiological situation in France. The phylogeography, time to the most recent common ancestor (TMRCA) and nucleotide substitution rate were studied using 118 full-length glycoprotein gene sequences isolated from 9 countries (5 genogroups) over a period of 47 years. The TMRCA dates back to 1943, with the L genogroup identified as the likely root (67 %), which is consistent with the first report of this pathogen in the USA. A Bayesian inference approach was applied to the partial...
Tipo: Text Palavras-chave: Infectious hematopoietic necrosis virus; Evolutionary dynamics; Dates of divergence; Phylogeography; Molecular epidemiology.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00399/51015/51832.pdf
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New insights about the introduction of the Portuguese oyster, Crassostrea angulata, into the North East Atlantic from Asia based on a highly polymorphic mitochondrial region ArchiMer
Grade, Ana; Chairi, Hicham; Lallias, Delphine; Power, Deborah M.; Ruano, Francisco; Leitao, Alexandra; Drago, Teresa; King, Jonathan W.; Boudry, Pierre; Batista, Frederico M..
It is commonly presumed that the Portuguese oyster Crassostrea angulata was introduced into the North East (NE) Atlantic from Asia. The analysis of the nucleotide sequence of a highly polymorphic non-coding mitochondrial region (major noncoding region - MNR) of C. angulata samples collected in Europe (Portugal), Africa (Morocco) and Asia (Shantou and Taiwan) provided new insight into the introduction of this species into the NE Atlantic. Sixty haplotypes and a nucleotide diversity of 0.0077 were observed in 130 analyzed sequences. Higher nucleotide diversity levels were observed in NE Atlantic sites than in Asian sites and significant genetic differentiation was found between the two. Our results suggest that C. angulata might have been introduced to the...
Tipo: Text Palavras-chave: Crassostrea angulata; Phylogeography; Genetic signature; Biological invasions.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00371/48200/48316.pdf
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Sharp genetic break between Atlantic and English Channel populations of the polychaete Pectinaria koreni, along the North coast of France ArchiMer
Jolly, Mt; Jollivet, D; Gentil, F; Thiebaut, E; Viard, F.
This study uses enzymatic and mitochondrial genes to infer the relative importance of historical processes and contemporary hydrodynamic features on the observed patterns of genetic structure in subdivided populations of Pectinaria koreni (Polychaeta: Pectinariidae) along the coasts of Brittany and the English Channel. Nucleotide sequence variation of a 603-bp fragment of the mtDNA cytochrome oxidase subunit I gene revealed a surprisingly deep phylogeographic break of about 16% divergence separating the Brittany and Channel populations, which coincides with a biogeographic boundary along the western coast of Brittany. Deep sequence divergence with fixed haplotype differences and the inversion of allele frequencies at two enzyme loci suggests the occurrence...
Tipo: Text Palavras-chave: Phylogeography; Allozymes; MtDNA; Parapatric boundary; Cryptic species.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/00301/41250/40433.pdf
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