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Registros recuperados: 60 | |
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Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky. |
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J... |
Tipo: Text |
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf |
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Berthe, Franck; Prou, Jean. |
French Polynesia is currently the world's largest producer of cultured black pearls with exports worth U5$150 millions annually. The activity has become of prime socioeconomic importance the archipelagos with strong support and control of the government. In the mid-1980s, mass mortality of the black-lipped pearl oyster, Pinctada margarltlfera, has occurred in several atolls and challenged the development of pearl farming activity. Consequent studies and surveys have described several pathological conditions. None of those is considered to pose significant threat to the industry. However, they are monitored by a surveillance program in order to prevent and control emerging diseases. The government of French Polynesia has developed efforts to maintain high... |
Tipo: Text |
Palavras-chave: Health management; Pinctada margaritifera; French Polynesia; Pathogens; Mass mortality. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3703.PDF |
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Pouvreau, Stephane; Tiapari, Jerome; Gangnery, Aline; Lagarde, Franck; Garnier, Matthieu; Teissier, Hinano; Haumani, G; Buestel, Dominique; Bodoy, Alain. |
Growth of the black-lip pearl oyster, Pinctada margaritifera var. cumingi, was studied for an annual cycle, from March 1997 to April 1998, in the lagoon of Takapoto atoll (Tuamotu archipelago, French Polynesia). Growth in shell and in tissue were measured every 15 days on three successive age groups of cultivated pearl oysters. At the same time, hydrobiological parameters (temperature, salinity, oxygen concentration, suspended particulate matter), known to have influence on bivalve growth, were followed each week during culture. No seasonal trend was observed in hydrobiological parameters, except for temperature which varied between 26 degrees C and 31 degrees C. The potential food for pearl oysters (particulate organic matter, POM, mg l(-1)) was slightly... |
Tipo: Text |
Palavras-chave: Pearl culture; Matter in suspension; Biochemical composition; Length weight relations ship; Tropical lagoon; Growth; Pinctada margaritifera. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-381.pdf |
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Ky, Chin-long; Broustal, Floriane; Potin, Dan; Lo, Cedrik. |
In French Polynesia, the P. margaritifera pearl aquaculture industry is spread over a vast area, as large as Europe. All the oysters for this the highly economically important activity are supplied from just a few collection lagoons, but they are grown in numerous sites across three archipelagos (Gambier, Society and Tuamotu). Many oyster transfers thus indirectly bring about grafting combinations mixing different geographic origins and production sites. This study aims to examine the impact of such graft combinations on cultured pearl quality traits. For this, six homogeneous and standardised experimental graft combinations (N = 6197) were conducted at commercial scale in the two growing locations the most frequently used in French Polynesia: Arutua atoll... |
Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Graft combinations; Pearl quality traits; Environment. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00477/58888/61444.pdf |
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Viard, Carole. |
L'industrie de la perle nmre étant florissante en Polynésie française, l'aquaculture de l'huître perlière Pinctada margaritifera (Linné, 1758) suscite de nombreuses recherches. L'objectif de cette étude était d'observer le contrôle qu'exerce la température sur la gamétogenèse de la nacre et de voir s'il était possible de synchroniser, grâce à ce paramètre, la maturité des individus en laboratoire. Les variations de températures annuelles observées dans le milieu naturel ont été reproduites sur huit semaines. Régulièrement, des lots de nacres ont été sacrifiés, plusieurs variables biométriques ont été mesurées et l'analyse histologique des gonades a permis d'identifier le stade de maturité. Les résultats ont montré que la température a eu une influence sur... |
Tipo: Text |
Palavras-chave: Huîtres perlières; Pinctada margaritifera; Température; Gamétogenèse; Conditionnement. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00446/55760/57421.pdf |
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Fournier, Jonathan; Dupuy, Celie; Bouvy, Marc; Couraudon-reale, Marine; Charpy, Loic; Pouvreau, Stephane; Le Moullac, Gilles; Le Pennec, Marie-annick; Cochard, Jean-claude. |
In atoll lagoons of French Polynesia, growth and reproduction of pearl oysters are mainly driven by plankton concentration. However, the actual diet of black-lip pearl oysters P. margaritifera in these lagoons is poorly known. To fill this gap, we used the flow through chamber method to measure clearance rates of P. margaritifera in Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia). We found : (i) that pearl oysters cleared plankton at a rate that was positively related to plankton biovolume, (ii) that nanoflagellates were the main source of carbon for the pearl oysters, (iii) that the quantity and origin of carbon filtrated by pearl oysters was highly dependent on the concentration and composition of plankton. These results provide essential... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Clearance rates; French Polynesia; Phytoplankton; Protists. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00078/18888/16599.pdf |
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Ky, Chin-long; Le Pabic, Lore; Koua, Manaarii Sham; Molinari, Nicolas; Nakasai, Seiji; Devaux, Dominique. |
The black-lipped pearl oyster, Pinctada margaritifera is the most important farmed mollusc species in French Polynesia. Donor oyster selection among wild P. margaritifera individuals, chosen according to their inner shell colour, makes it possible to obtain the broadest range of cultured pearl colours of any species. This study demonstrates the relative influence of using black [B] or red [R] outer shell phenotypes, combined with green [G] or yellow [Y] inner shell phenotypes, on pearl darkness level, colour categories and lustre. A large scale grafting experiment was designed and carried out over five grow-out locations, covering three archipelagos: Tuamotus, Society and Gambier. Results revealed that the [B + G] phenotypes may be used as donors to... |
Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Shell colour phenotype; Pearl colour; Environment. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00285/39637/38129.pdf |
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Tayale, Alexandre; Gueguen, Yannick; Treguier, Cathy; Le Grand, Jacqueline; Cochennec-laureau, Nathalie; Montagnani, Caroline; Ky, Chin-long. |
Producing high quality cultured black pearls from Pinctada margaritifera is one of the major challenges for the "pearl oyster" industry in French Polynesia. In order to assess donor effect on cultured pearl quality, wild Pinctada margaritifera originating from the Tuamotu Archipelago were used in a duplicated grafting experiment. After 12 months of culture, nucleus retention was assessed and seven pearl quality traits recorded on the 454 cultured pearls harvested from the experiment. The traits scored were nacre thickness and pearl weight, surface defects, lustre, grade, and the colour components: 1) darkness of cultured pearl colour, and 2) visual perception of colour class (bodycolor and/or overtone). Our results demonstrate for the first time that... |
Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Nacre; Donor mollusc; Genetic selection; Pteriidae; Blacklip pearl oyster; Pinctada margaritifera; French Polynesia. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00116/22757/20541.pdf |
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Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Beliaeff, Benoit; Andrefouet, Serge. |
In French Polynesia black pearl farming represents one of the dominant business sectors. However, it still entirely relies on unpredictable Pinctada margaritifera spat collection success, which is itself conditioned by larval development completion. To assess the relationship between larval development and recruitment success, we studied under controlled conditions the effect of food concentration on development, growth, ingestion rate, survival and metabolic rate at the larval stage. Larvae were exposed to four different phytoplankton densities (2,5; 7,5; 15 and 30 cell.μL−1). Larvae survived equally all over the range of phytoplankton concentration with an average survival rate of 16% at the end of experiments. Food concentration significantly affected... |
Tipo: Text |
Palavras-chave: Bivalve larvae; Pinctada margaritifera; Physiology; Energetics; Dynamic energy budget theory; Pearl farming; Aquaculture. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00476/58795/61446.pdf |
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Cochennec-laureau, Nathalie; Montagnani, Caroline; Saulnier, Denis; Fougerouse, Angelique; Levy, Peva; Lo, Cedrik. |
Pearl oyster grafting is a complex surgical operation that should lead to pearl formation after approximately eighteen months. Although this technique has been used for many years in French Polynesia, the grafting process is still not standardised. While studies have been carried out in order to improve graft performance and yield, these remain highly variable due to post-grafting mortality, nucleus rejection and unreliable pearl quality, all of which constrain pearl farm profitability. The present study uses histological analysis to monitor oysters that either rejected or retained their nuclei. Both groups of oysters are compared in terms of evolution of the graft, which could influence retention, and the development of a pearl sac in cases where grafting... |
Tipo: Text |
Palavras-chave: Grafting process; Pearl formation; Pearl oyster; Pteriidae; Pinctada margaritifera. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11288/7823.pdf |
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Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe. |
Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of... |
Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf |
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Comps, Marie-annick; Herbaut, Christian; Fougerouse, Angélique. |
Whitish parasite-like bodies (PLBs), 0.5-2 mu m in size, were detected within the pearl bag of some grafted pearl oysters, Pinctada margaritifera, from Takapoto island (French Polynesia). Histological and ultrastructural investigations have revealed that these bodies consisted of lamellar organic material locally secreted by some epithelial cells of the pearl bag. The fine structure and the formation of these bodies are described. The causes of this abnormal secretion of organic mater, which actually corresponds to periostracum, are not presently known. No parasite nor infectious micro-organism was found associated with the PLBs. |
Tipo: Text |
Palavras-chave: Periostracum; Pearl bag; Pinctada margaritifera. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-545.pdf |
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Lacoste, Elise; Le Moullac, Gilles; Levy, Peva; Gueguen, Yannick; Gaertner-mazouni, Nabila. |
In bivalve aquaculture, dominant fouling organisms are filter feeders which can compete for food with reared bivalves, sometimes causing mortality or reducing their growth rate. This study investigated the effect of biofouling on the farmed pearl oyster Pinctada margaritifera in two lagoons of French Polynesia. Survival, growth and reproduction of 2 year-old pearl oysters were monitored with regular sampling schedules, from the initial stage of colonization up to 20 months of biofouling accumulation. Control groups of pearl oysters were kept free of biofouling as is the current practice in pearl farms. After more than a year of monitoring, no significant difference was recorded in shell growth rate between pearl oysters reared with epibionts and the... |
Tipo: Text |
Palavras-chave: Pearl oyster culture; Pinctada margaritifera; Biofouling; Growth; Reproduction. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31188/29587.pdf |
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Ky, Chin-long; Nakasai, Seiji; Molinari, Nicolas; Devaux, Dominique. |
Cultured pearls quality produced by the mollusc Pinctada margaritifera var. cumingi is determined by a panel of criteria, of which shape is one of the most relevant. Cultured pearls with round and semi-round (RS) shapes are the most valuable. Decreasing the proportion of other pearl shapes (OT: baroque, button, drop, oval), and especially the circled pearls (CL) and rejects (rebuts: RT), is an important challenge for the pearl industry. The present study examined the effect of grafter skill and season of graft on the variation in proportions of CL, RS, OT and RT cultured pearl shape categories in a mono-site culture (Mangareva island lagoon). Six large-scale grafting experiments, carried out in the same way as commercial grafting sessions, were made by... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl; Grafter skill; Pearl shapes; Circled pearls; Grafting season. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00216/32745/31150.pdf |
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Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Lo, Cédrik; Cabral, Philippe. |
The top aquaculture species in French Polynesia is Pinctada margaritifera, a mollusc grown for the production of a unique gem: the black pearl. One of the challenges facing the pearl farming industry is to "produce less but better pearls" through genetic improvement. An experimental hatchery system was used to generate full-sib families to be tested for their potential as donor "oysters". A large-scale grafting experiments was done and seven cultured pearl quality traits: grade, surface defects, lustre, darkness level, visual colour categories, circles and shape categories were recorded. Our results revealed, for the first time, significant phenotypic relationships between these quality traits. The grade A cultured pearl class had the largest proportion of... |
Tipo: Text |
Palavras-chave: Pearl "oyster"; Pinctada margaritifera; Donor "oyster"; Cultured pearl quality; Pearl grade; Pearl colour; Pearl shape; Selective breeding. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30130/28592.pdf |
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Ky, Chin-long; Le Moullac, Gilles. |
Size is the most important and valuable quality trait of cultured pearls produced by the black-lipped pearl oyster, Pinctada margaritifera. In French Polynesia, several breeding programmes have been started that aim to improve this size trait, which is highly related to shell growth rate in both recipient and donor oysters. Shell growth rate dictates the time of grafting, size of implanted nuclei and bio-mineralisation potential of the mantle and pearl sac. We assessed shell growth rate through routine digital shell biometric analysis on 22 hatchery families produced between 2005 and 2008. These included full-sib families and half-sib families derived from polyandry (one dam crossed with two or more sires). Results showed that: 1) a significant family... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Hatchery-produced families; Growth performance; Shell diameter; Cultured pearl size. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49810/50384.pdf |
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Gueguen, Yannick; Saulnier, Denis; Santini, Adrien; Magre, Kevin; Garen, Pierre; Bernagout, Solene; Nohl, Marine; Bouisset, Patrick; Helme, Herehia; Planes, Serge; Le Moullac, Gilles. |
This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM,... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; French Polynesia; Cadmium; Chromium; Gene expression; Metallothionein. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00376/48707/49146.pdf |
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Registros recuperados: 60 | |
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