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| Registros recuperados: 16 | |
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| CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; ALEXANDRE,RODRIGO S.. |
| Abstract This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L; Malt extract; Myo-inositol; Polyethylene glycol. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703433 |
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| GOUVEA,DRIELLY S.; CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; SCHMILDT,OMAR; ARAUJO,CAROLINE P. DE; ALEXANDRE,RODRIGO S.. |
| Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Auxins; Carica papaya L.; Gibberellic acid; Hermaphrodite explants; Polyethylene glycol. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500856 |
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| Lucena,Antônio Edson de Souza; Sampaio,Divaldo de Almeida; Silva,Ednaldo Rosas da; Paiva,Virgínia Florêncio de; Santiago,Ana Cláudia; Leite,Ana Cristina Lima. |
| Highly purified intravenous immunoglobulin G concentrate (IV IgG) was produced with the use of polyethylene glycol associated to a single-stage precipitation by ethanol, instead of the classic Cohn-Oncley process, which employs cold alcohol as the precipitating agent, in a three-stage process. Precipitation of crude fraction containing more than 95% of immunoglobulin G was performed by liquid chromatography with a cation exchanger, CM-Sepharose, as a stationary phase. During the process, the product was subjected to two-stage viral inactivation. The first stage was performed by the action of sodium caprylate, 30 mM at pH 5.1+/- 0.1, and the second stage was performed by the action of a solvent-detergent mixture. The finished product was formulated at 5%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood derivatives; Immunoglobulins; Polyethylene glycol; Ultrafiltration; Ion exchange chromatography. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400020 |
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| Santos,João Henrique Picado Madalena; Torres-Obreque,Karin Mariana; Meneguetti,Giovanna Pastore; Amaro,Beatriz Panichi; Rangel-Yagui,Carlota Oliveira. |
| The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PEGylation; Biobetters; Biological drugs; Polyethylene glycol; Protein purification; Site-specific PEGylation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700408 |
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| Hussain,Amjad; Mumtaz,Ahmad Mehmood; Arshad,Muhammad Sohail; Abbas,Nasir; Latif,Abida; Shamim,Rahat; Bukhari,Nadeem Irfan; Hussain,Khalid. |
| ABSTRACT The present study describes the development of theophylline microcapsules by a non-solvent addition method and the effect of plasticizer addition on microencapsulation. The release was studied in distilled water and the data were analysed by various mathematical models for determining the mechanism of release. Prepared microcapsules were found to be spherical, free flowing and having more than 80% entrapped drug. The polymer - cellulose acetate phthalate and plasticizer - polyethylene glycol was considered to be affecting the properties of microcapsules including drug release (time for 50% drug release, T50). The formulation with the highest proportion of polymer and without plasticizer (F3) showed the slowest release with T50 = 4.3 h, while the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Theophylline/microcapsules; Microencapsulation; Cellulose acetate phthalate; Polyethylene glycol; Microcapsules/mechanism of release; Drugs/release.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100004 |
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| Faria,José M.R.; Davide,Lisete C.; Silva,Edvaldo A. A. da; Davide,Antonio C.; Pereira,Roselaine C.; van Lammeren,André A.M.; Hilhorst,Henk W.M.. |
| The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5ºC fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Polyethylene glycol; Recalcitrant seeds; Seed conservation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400008 |
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| Silva,Martha Freire da; Araújo,Eduardo Fontes; Silva,Laércio Junio da; Amaro,Hugo Tiago Ribeiro; Dias,Luiz Antônio dos Santos; Dias,Denise Cunha Fernandes dos Santos. |
| ABSTRACT Crambe is an oilseed plant whose oil is suitable for various industrial purposes, such as production of biodiesel, plastics, rubbers, and cosmetics. There are reports of the rusticity of this species and its potential for growth in arid and semi-arid environments, where water scarcity and soil salinity are common. However, studies regarding the tolerance of its seeds to these stresses and the physiological responses triggered during germination and seedling establishment under these conditions are incipient. The objective of this study was to evaluate the tolerance of crambe seeds to water deficit and salt stress during germination and early seedling development, as well as to investigate the role of antioxidant enzymes on tolerance to these... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abiotic stresses; Antioxidant enzymes; Polyethylene glycol; Potassium chloride; Oilseed plant.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100201 |
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| Bortolin,Gabriel Streck; Salinas-Arcienega,Alfredo José; Galviz-Fajardo,Yutcelia Carolina; Amarante,Luciano do; Pedroso,Carlos Eduardo da Silva; Oliveira,João Carlos Pinto; Köpp,Mauricio Marini; Martins,Andrea Bicca Noguez; Tunes,Lilian Vanussa Madruga de. |
| ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paspalum notatum Flügge; Ecotype; Polyethylene glycol; Drought stress; Oxidative stress. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500202 |
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| GLORIA,FERNANDA JANUZZI MENDES DA; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES; MENDES,BEATRIZ MADALENA JANUZZI. |
| A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultivar improvement; Polyethylene glycol; Rootstock; Tissue culture. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400008 |
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| Pereira,Isabella C.; Catão,Hugo C. R. M.; Caixeta,Franciele. |
| ABSTRACT The aim of this study was to evaluate seed physiological quality and initial pea (Pisum sativum) seedling development at different water and salt potentials. Polyethylene glycol 6000, sodium chloride and potassium chloride solutions with different osmotic potentials (0; -0.2; -0.4; -0.6; -0.8; -1.0, and -1.2 MPa) were used, and the experiment had a 3 × 7 factorial arrangement. The physiological quality and initial seedling growth characteristics (first germination count, final germination count, strong normal seedlings, epicotyl and taproot lengths, epicotyl dry matter, and root dry matter) were evaluated in a completely randomized experimental design, with four repetitions. Decreases in the observed variables showed that the simulated stress... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pisum sativum; Vegetable; Polyethylene glycol; Osmotic potential. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662020000200095 |
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| Machado,Fernando H. B.; David,Andréia M. S. de S.; Cangussú,Lucas V. S.; Figueiredo,Josiane C.; Amaro,Hugo T. R.. |
| ABSTRACT Water stress is a condition that causes physiological changes in different species and even genotypes of the same species. One of the osmotic agents most used to simulate this condition is polyethylene glycol 6000. The aim of this study was to evaluate the effects of water stress on the physiological quality of seeds and performance of seedlings of crambe genotypes. A completely randomized design was used, in a factorial scheme with two genotypes (one cultivar and one line) and five osmotic potentials simulated with aqueous solutions of polyethylene glycol 6000 (control = 0, -0.25, -0.50, -1.0, -1.50 MPa), with four replicates of 50 seeds per treatment. The effects of the treatments were evaluated by means of germination, first count, germination... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crambe abyssinica Hoechst; Germination; Polyethylene glycol. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000300175 |
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| Paiva,Emanoela P. de; Sá,Francisco V. da S.; Torres,Salvador B.; Brito,Marcos E. B.; Moreira,Romulo C. L.; Silva,Luderlândio de A.. |
| ABSTRACT The objective of this study was to evaluate the tolerance of cowpea cultivars to water stress, during seed germination and seedling establishment. For this, a completely randomized experimental design was used in a factorial scheme consisting of nine cultivars of cowpea (BRS Guariba, BRS Potengi, BRS Itaim, BRS 17 Gurguéia, BRS Aracê, Paulistinha, Maratanã, Costela-de-Vaca and Canapu-Branco) and two levels of osmotic potential induced by PEG 6000 (0.0 MPa (control) and -0.4 MPa) in four replicates of 50 seeds. The variables analysed were germination, germination first count, root and shoot lengths, and dry matter accumulation of shoots and roots. The dissimilarity between the genotypes was also determined by Euclidean distance. Germination and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Initial growth; Polyethylene glycol; Osmotic potential. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000600407 |
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| Registros recuperados: 16 | |
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