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Última Seleção de leveduras da Região Amazônica para produção de protease extracelular 36 Neves,Kilma Cristiane Silva; Porto,Ana Lúcia Figueiredo; Teixeira,Maria Francisca Simas. O objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10(6) UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte. Tipo: Info:eu-repo/semantics/article Palavras-chave: Leveduras; Região Amazônica; Protease. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672006000300002 Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L.) - doi: 10.4025/actasciagron.v33i3.6734 1 Rodarte, Mirian Pereira; Ufla; Dias, Disney Ribeiro; UNILAVRAS - Centro Universitário de Lavras; Vilela, Danielle Marques; Ufla; Schwan, Rosane Freitas; Ufla. One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea Arabica) were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positive isolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH values (5.0, 7.0 and 9.0) at 30 oC. Bacillus megaterium, B. subtilis, Enterobacter agglomerans, Kurthia sp, Pseudomonas paucimobilis and Tatumella ptyseos demonstrated the highest proteolytic activities at pH 9.0. One yeast isolate, Citeromyces matritensis, had a proteolytic activity of 2.40 U at pH 5.0. Aspergillus dimorphicus, A. ochraceus, Fusarium moniliforme, F.... Tipo: Pesquisa de campo; Laboratório Palavras-chave: 5.01.02.04-4 microbial enzyme; Protease; Coffee fermentation Microbiologia Agrícola microbial enzyme; Protease; Coffee fermentation. Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6734 Schistosome serine protease inhibitors: parasite defense or homeostasis? 42 Quezada,Landys A. Lopez; McKerrow,James H. Serpins are a structurally conserved family of macromolecular inhibitors found in numerous biological systems. The completion and annotation of the genomes of Schistosoma mansoni and Schistosoma japonicum has enabled the identification by phylogenetic analysis of two major serpin clades. S. mansoni shows a greater multiplicity of serpin genes, perhaps reflecting adaptation to infection of a human host. Putative targets of schistosome serpins can be predicted from the sequence of the reactive center loop (RCL). Schistosome serpins may play important roles in both post-translational regulation of schistosome-derived proteases, as well as parasite defense mechanisms against the action of host proteases. Tipo: Info:eu-repo/semantics/article Palavras-chave: Schistosomiasis; Inhibitor; Protease; Serpin; Genome. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200025 The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function 42 Santos,André L.S.; Branquinha,Marta H.; D'Avila-Levy,Claudia M.. Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of Leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosomatidae; Lower trypanosomatids; Gp63; Invertebrate host; Adhesion; Nutrition; Protease. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006 Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil 42 Santos,Anderson F.; Pires,Fabiano; Jesus,Hugo E.; Santos,André L.S.; Peixoto,Raquel; Rosado,Alexandre S.; D'Avila-Levy,Claudia M.; Branquinha,Marta H.. Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Algoriphagus antarcticus; Antarctica; Sporosarcina aquimarina; Protease. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109 Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte 45 Moura,F.A.S.; Dourado,L.R.B.; Farias,L.A.; Lopes,J.B.; Lima,S.B.P.; Fernandes,M.L.. RESUMO Objetivou-se avaliar o efeito de complexos enzimáticos sobre a energia metabolizável e o coeficiente de digestibilidade de nutrientes do milheto para frangos de corte. Quinhentos e setenta e seis frangos machos foram distribuídos em 36 gaiolas, com três tratamentos: T1 - composição de milheto sem complexo enzimático; T2 - composição de milheto com complexo enzimático (CES) e T3 - composição de milheto com complexo enzimático (CEV). Os tratamentos foram definidos com base em seis dietas (três dietas referências e três dietas testes). As dietas testes foram obtidas pela substituição de 40% da dieta referência por milheto inteiro, e a adição de enzimas consistiu de dois complexos enzimáticos: CES, constituído pelas enzimas fitase, protease, xilanase,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alimento alternativo; Dietas; Enzimas; Protease; Xilanase. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300990 Efeito da protease sobre o coeficiente de metabolizabilidade dos nutrientes em frangos de corte 45 Matias,C.F.Q.; Rocha,J.S.R.; Pompeu,M.A.; Baião,R.C.; Baião,N.C.; Lara,L.J.C.; Clímaco,W.L.S.; Pereira,L.F.P.; Caldas,E.O.; Teixeira,M.P.F.; Cardeal,P.C.. Avaliaram-se os efeitos da protease sobre o coeficiente de metabolizabilidade dos nutrientes em dietas contendo farinha de penas (2,0% na fase inicial e 3,0% na fase de crescimento) para frangos de corte machos, Cobb(r), de um a 32 dias de idade. Foram alojadas 336 aves em gaiolas metálicas equipadas com bandeja para coleta total de excretas. Foi utilizado delineamento inteiramente ao acaso, em arranjo fatorial 2x2 (com e sem adição da enzima protease 0,05% e duas valorizações da matriz nutricional da enzima), sendo sete repetições por tratamento, de 12 aves cada. Observou-se, na fase inicial, maior CMPB (P≤0,05) para as aves que, independentemente da adição de protease, consumiram ração com valorização da matriz nutricional da enzima. Não houve efeito dos... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coeficiente de metabolizabilidade; Enzimas exógenas; Frango de corte; Protease. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200492 Nematicidal activity of Paecilomyces marquandii proteases on infective larvae of Ancylostoma spp 52 Genier,Hugo Leonardo André; Queiroz,José Humberto de; Braga,Fabio Ribeiro; Soares,Filippe Elias de Freitas; Araújo,Jackson Victor de; Pinheiro,Iara Rebouças. ABSTRACT The present study aimed to evaluate the action of Paecilomyces marquandii proteases on Ancylostoma spp L3. White halos in the zymogram confirmed the proteolytic action. Difference (p <0.01) between the number of L3 in the differents groups was found, with 41.4% of reduction of Ancylostoma spp. L3 before 24 hours. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Nematophagous fungi; Protease; Zoonosis. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100423 Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae 52 Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain. In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175 Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity 52 Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de. The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003 Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents 52 Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil. ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462 Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation 52 Siqueira,Ana Claudia Rodrigues de; Rosa,Nathalia Gonsales da; Motta,Cristina Maria Souza; Cabral,Hamilton. The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the crude enzymatic extract showed the optimum pH and temperature of 6.5 and 55°C, respectively. The stability at different temperatures and pH values showed that the extract could endure different pH. The evaluation of the ions influence and inhibitors proved that the enzyme required an ion for better activity, which was corroborated with the inhibition of EDTA and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Solid-state fermentation; Keratinase; Wheat bran; Aspergillus terreus. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400514 Prevalence of resistance-associated mutations in Human Immunodeficiency Virus type 1-positive individuals failing HAART in Rio de Janeiro, Brazil 55 Varella,Rafael Brandão; Ferreira,Selma Baía; Castro,Márcia Braga de; Tavares,Marisa Dias; Zalis,Mariano Gustavo. We investigated the occurrence of HIV-1 antiretroviral resistance in individuals failing to respond to highly active antiretroviral therapy (HAART) attended by RENAGENO from 2001-2004. One hundred and seventeen patients were selected for this study; their plasma viral RNA was extracted and the PR and RT genes sequenced to examine subtype, genetic polymorphisms and mutations associated with resistance to antiretroviral drugs. HIV-1 sequence analysis showed that 86/100 (86%) were infected with subtype B, 7/100 (7%) with subtype F and 7/100 (7%) with RT/PR hybrid forms (2 D/B, 2 F/B, 2 B/F and 1 D/F). In 14 (12%) of the samples, the subtype was not determined. The prevalence of resistance mutations was high (93.1%), mainly in the RT gene. The most prevalent... Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV-1; HAART; Reverse transcriptase; Protease; Resistance. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500007 Functions of the extracellular matrix and matrix degrading proteases during tumor progression 56 Liaw,L.; Crawford,H.C.. Cell interactions with extracellular matrices are important to pathological changes that occur during cell transformation and tumorigenesis. Several extracellular matrix proteins including fibronectin, thrombospondin-1, laminin, SPARC, and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration, survival, or angiogenesis. Likewise, proteases including the matrix metalloproteinases (MMPs) are understood to not only facilitate migration of cells by degradation of matrices, but also to affect tumor formation and growth. We have recently demonstrated an in vivo role for the RGD-containing protein, osteopontin, during tumor progression, and found evidence for distinct functions in the host versus the tumor cells. Because of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Matrix metalloproteinase; Osteopontin; Carcinoma; Protease. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700002 Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent 58 Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal. Enzymes, and particularly proteases, have become an important and indispensable part of industrial processes such as laundry detergents, pharmaceuticals and food products. Detergents such as Tide®, Ariel® and Biz® contain proteolytic enzymes, most of them produced by members of the genus Bacillus. This paper describes the compatibility of protease produced by the thermophilic Bacillus sp, with commercial laundry detergent. Stability studies indicated that this enzyme retained about 95% and 74% of its maximum activity after 1h at 60ºC in the presence of glycine in combination with MnSO4 and CaCl2, respectively. No inhibitory effect was observed at 1.0-5.0 mM of EDTA. Triton X-100 inhibited the enzyme in all the concentrations tested. The enzyme was unstable... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.; Detergents. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300020 Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures 58 Moreira,Fabiana Guillen; Reis,Simone dos; Costa,Maria Aparecida Ferreira; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina. The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC. Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrases; Hydrolytic enzymes; Myrothecium verrucaria; Protease. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100002 Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease 58 Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo. Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen. have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase, polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%). Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009 Production and properties of an extracellular protease from thermophilic Bacillus sp 58 Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal. Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing trisodium citrate reached a maximum in 9h, with levels of 1.93U/mg protein. The microorganism utilized several carbon sources for the production of protease. Starch was the best substrate, followed by trisodium citrate, citric acid and sucrose. Among the various organic and inorganic nitrogen sources, ammonium nitrate was found to be the best. Studies on the protease characterization revealed that the optimum temperature of this enzyme was 60ºC. The enzyme was stable for 2h at 30ºC, while at 40ºC and 80ºC, 14% and 84% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be 8.0. After incubation of crude enzyme... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100015 Effect of the culture conditions on the production of an extracellular protease by thermophilic Bacillus sp and some properties of the enzymatic activity 58 Silva,Camila Rocha da; Delatorre,Andréia Boechat; Martins,Meire Lelis Leal. Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing 1% maltose as a carbon source and supplemented with whey protein (0.1%) and corn steep liquor (0.3%) reached a maximum at 14 h, with levels of 42 U/mg protein. The microorganism was capable of utilizing a wide range of carbon sources, but protease activity varied according the carbon source. Starch and maltose were the best carbon sources in the present study for protease secretion, while lactose and sucrose were less effective. Increasing maltose concentration in the medium until 1%, improved the growth of the organism and the enzyme activity. Regarding the amounts of corn steep liquor and whey protein in the medium, the concentrations of 0.2% and 0.1%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200012 Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach 58 Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M.. Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. Tipo: Info:eu-repo/semantics/article Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design (CCD). Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 Registros recuperados: 58 Primeira ... 1 2 3 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco