|
|
|
| Registros recuperados: 53 | |
|
| |
|
| |
|
| |
|
| |
|
|
| Biagioli,Carolina; Rios,Raúl; Basso,Alicia; Franzone,Pascual; Prina,Alberto. |
| Although it is generally accepted that plant in vitro culture response is influenced by the donor genotype, the genetic and molecular bases of this phenomenon are barely known. As a consequence, the optimization of tissue culture protocols is mainly empirically done. Researchers of the IGEAF studied the genetic basis of the in vitro regeneration of various plant species, including the tissue culture response of artificially induced barley mutants. One barley mutant, MC 169, carries a nuclear gene, recently described controlling the root growth in hydroponic cultivation. Under this condition, the roots of MC 169 mutant plants were longer than those of the original wild type line MC 182, a fact that was associated with a reduced ethylene biosynthesis. On the... |
| Tipo: Journal article |
Palavras-chave: Callus induction; Ethylene; Habituated calli; Hydroponic culture; Regeneration. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300019 |
| |
|
| |
|
|
| Zhao,Qing; Jing,Ji; Wang,Gang; Wang,Jie Hua; Feng,Yuan Yuan; Xing,Han Wen; Guan,Chun Feng. |
| Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; Gene transfer; Green fluorescent protein; Regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500009 |
| |
|
|
| González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela. |
| Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC... |
| Tipo: Journal article |
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009 |
| |
|
| |
|
|
| Scotton,Danielle C; Benedito,Vagner Augusto; Molfetta,Jeanne B de; Rodrigues,Benedita Inês FP; Tulmann-Neto,Augusto; Figueira,Antonio. |
| Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium sativum; Bulb; Callus induction; Organogenesis; Regeneration; Tissue culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100013 |
| |
|
| |
|
| |
|
|
| Cossa, Daniel; Cotte Krief, Marie-hélène; Mason, Robert P.; Bretaudeau-sanjuan, Jane. |
| The purpose of this paper is to investigate the distribution and the relative importance of processes that affect mercury distribution in the water column at the shelf edge of the Celtic Sea, on the western European continental margin. The water column, down to 4500 m, was sampled during two cruises, one in winter 1994 and the other in June 1995, on eight stations. Total mercury concentrations ranged from 0.29 to 9.37 pM with the extreme values in the first 200 m. The highly variable concentrations in the surface layers are attributed to intense atmospheric-sea water exchange processes. The low concentrations encountered in the shelf water prelude any significant influence of continental waters in this area. In the mixed layer, biological uptake and... |
| Tipo: Text |
Palavras-chave: Regeneration; Atmospheric deposition; Deep profile; Upwelling; Atlantic ocean; Celtic Sea; Shelf break; Continental margin; Mercury. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-660.pdf |
| |
|
| |
|
|
| da Silva,Jairo Pinheiro; de Carvalho,Camila Albuquerque Mello; Romualdo,Andressa Rodrigues; Schiavoni,Vagner Sarraipo; Barbosa,Marcello Henrique Nogueira; de Moraes,Marcella Suélen Torrecilla; Tirapelli,Daniela Petri Cunha; Fazan,Valéria de Paula Sassoli; Thomazini,José Antônio; Tirapelli,Luis Fernando. |
| The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w 4 J/cm and 100 w 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone... |
| Tipo: Journal article |
Palavras-chave: Bone defect; Diabetes mellitus; Laser therapy; Regeneration; Apoptosis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100029 |
| |
|
|
| Koike,Tatiana Emy; Camargo,Regina Celi Trindade; Ozaki,Guilherme Akio Tamura; Castoldi,Robson Chacon; Seraphim,Patrícia Monteiro; Oikawa,Sérgio Minoro; Camargo Filho,José Carlos Silva. |
| The aim of this study was to analyze the effects of an association of cryotherapy and therapeutic ultrasound on the treatment of muscle injured by impact. Fifty-five Wistar rats were divided into five groups (n = 11), Acute Injury (AI), Injury (I), Cryotherapy (CR), Therapeutic Ultrasound (TU) and Association of Cryotherapy and Therapeutic Ultrasound (CRTU). The CR and CRTU groups received applications of Cryotherapy three times (immediately, 24 and 48 h after injury) of 20 minutes duration. The TU and CRTU groups received applications of Therapeutic Ultrasound for seven days, for five minutes, in pulsed mode, 0.5 w/cm intensity, frequency 1 MHz. Body mass and gastrocnemius mass were analyzed. In addition to histological slides stained with hematoxylin and... |
| Tipo: Journal article |
Palavras-chave: Fractal; Regeneration; Cryotherapy; Ultrasound Therapy; Collagen. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300042 |
| |
|
| |
|
| |
|
| |
|
|
| Ksouri, J.; Mensi, F.; Salhi-Hamza, K.. |
| Afin d’évaluer le potentiel de régénération de Gracilaria verrucosa du lac de Bizerte, une culture expérimentale, in situ sur cordes tendues sur le substrat, a été mise en place à partir de boutures de 200 g. Au terme de quatre mois de culture, de février à mai 2003, les cordes ont été soumises à une, deux, trois ou quatre coupes. L’analyse statistique montre que la meilleure situation est représentée par les touffes qui n’ont subi qu’une seule coupe à la fin du mois de mai; dans ce cas, ces touffes ont un poids final de 1771,4 g et un taux de croissance spécifique de 1,8. |
| Tipo: Journal Contribution |
Palavras-chave: Aquaculture systems; Cutting; Experimental culture; Regeneration; Seaweed culture; Gracilaria verrucosa; Http://aims.fao.org/aos/agrovoc/c_49675. |
| Ano: 2006 |
URL: http://hdl.handle.net/1834/4253 |
| |
| Registros recuperados: 53 | |
|
|
|
