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Registros recuperados: 35
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Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis Electron. J. Biotechnol.
Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu.
A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize...
Tipo: Journal article Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007
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Plant growth-promoting bacteria as inoculants in agricultural soils Genet. Mol. Biol.
Souza,Rocheli de; Ambrosini,Adriana; Passaglia,Luciane M.P..
Abstract Plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. Plant growth-promoting bacteria (PGPB) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, ACC deaminase activity, and production of siderophores and phytohormones, can be assessed as plant growth promotion (PGP) traits. Bacterial inoculants can contribute to increase agronomic efficiency by reducing production costs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen fixation; Siderophore production; Plant-bacteria interaction; Inoculant; Rhizosphere.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400401
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Disponibilização de K de rochas silicáticas para plantas de soja inoculadas com fungos micorrízicos arbusculares (FMA). Infoteca-e
ANDRADE, L. R. M. de; FALEIRO, A. S. G.; SANTOS SOBRINHO, D. A.; NASCIMENTO, M. T. DO; SOUZA, A. L. DE.
bitstream/item/75813/1/bolpd-303.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Fungo micorrízico arbuscular; Nutrientes; Arbuscular mycorrhizal fungus.; Rizosfera; Soja.; Nutrients; Soybeans.; Rhizosphere.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/931541
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Effect of plant photosynthesis, carbon sources and ammonium availability on nitrogen fixation rates in the rhizosphere of Zostera noltii ArchiMer
Welsh, Dt; Bourgues, S; Dewit, R; Auby, Isabelle.
Rates of nitrogen fixation (measured as acetylene reduction) in the rhizosphere of the seagrass Zostera noltii were highly dependent upon plant photosynthetic activity being significantly stimulated at elevated CO2 concentrations and by light, both in the short-term and over diurnal cycles. Stimulation by light became insignificant when 5 mM sucrose was added to the sediment porewater, indicating that in the absence of added carbon sources, light stimulation was due to direct inputs of plant photosynthate to the rhizosphere. Addition of a range of carbon sources to the rhizosphere sediment stimulated rates of acetylene reduction, with this stimulation being significant for sucrose and lactate. Surprisingly, whilst low additions of ammonium to the sediment...
Tipo: Text Palavras-chave: Acetylene reduction; Nitrogen fixation; Rhizosphere; Zostera noltii; Plant photosynthesis; Root exudates; Carbon sources; Ammonium.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00151/26266/24350.pdf
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MECHANISMS AND PRACTICAL CONSIDERATIONS INVOLVED IN PLANT GROWTH PROMOTION BY RHIZOBACTERIA J. Soil Sci. Plant Nutr.
Martínez-Viveros,O; Jorquera,M.A; Crowley,D.E; Gajardo,G; Mora,M.L.
Rhizobacteria are capable of stimulating plant growth through a variety of mechanisms that include improvement of plant nutrition, production and regulation of phytohormones, and suppression of disease causing organisms. While considerable research has demonstrated their potential utility, the successful application of plant growth promoting rhizobacteria (PGPR) in the field has been limited by a lack of knowledge of ecological factors that determine their survival and activity in the plant rhizosphere. To be effective, PGPR must maintain a critical population density of active cells. Inoculation with PGPR strains can temporarily enhance the population size, but inoculants often have poor survival and compete with indigenous bacteria for available growth...
Tipo: Journal article Palavras-chave: Agricultural inoculant; Phytohormone; Phytopathogen biocontrol; Plant nutrition; Rhizosphere.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100006
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Effect of Bacillus thuringiensis on microbial functional groups in sorghum rhizosphere PAB
Brasil,Carlos; Matsumoto,Leopoldo Sussumu; Nogueira,Marco Antonio; Spago,Flavia Regina; Rampazo,Luís Gustavo; Cruz,Marcio Ferreira; Andrade,Galdino.
The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500022
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Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
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Silicon-mediated alleviation of Cadmium toxicity on Tujopsis dolabrata Phyton
Huang,YC; Chen,H; Zhao,WJ; Li,WD; Yang,HY; Sun,Y; Wang,L; Cao,SH.
We conducted pot experiments on the cypress Thujopsis dolabrata (Linn. f.) Sieb. et Zucc. in order to study the interaction of silicon (Si) and root exudates on cadmium (Cd) bioavailability in the rhizosphere,. Each variety was planted with 100 mg/kg Cd and/or 400 mg/kg Si for 210 days. The results showed that adding Si increased Cd tolerance in T. dolabrata, but that the mechanism was specifical. In T. dolabrata, Si did not prevent Cd translocation from roots to shoots, and it significantly enhanced Cd accumulation without inhibiting growth. Moreover, Si mobilized Cd from the rhizospheric soil by stimulating phenolic exudation from the roots, suggesting that Cd-chelation combined with Si-induced phenolics were involved in Cd detoxification.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Rhizosphere; Root exudation; Silicon; Thujopsis dolabrata.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200016
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Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays) Phyton
Vital-López,L; Cruz-Hernández,MA; Fernández-Dávila,S; Mendoza-Herrera,A.
Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Glufosinate; Metagenomic DNA; Rhizosphere; Single-strand conformation polymorphism.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200006
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Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis Phyton
Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
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Plant availability of trace elements in sewage sludge-treated soils: methodology¹ Rev. Bras. Ciênc. Solo
Marchi,Giuliano; Guilherme,Luiz Roberto Guimarães; Chang,Andrew C.
Synthetic root exudates were formulated based on the organic acid composition of root exudates derived from the rhizosphere of aseptically grown corn plants, pH of the rhizosphere, and the background chemical matrices of the soil solutions. The synthetic root exudates, which mimic the chemical conditions of the rhizosphere environment where soil-borne metals are dissolved and absorbed by plants, were used to extract metals from sewage-sludge treated soils 16 successive times. The concentrations of Zn, Cd, Ni, Cr, and Cu of the sludge-treated soil were 71.74, 0.21, 15.90, 58.12, and 37.44 mg kg-1, respectively. The composition of synthetic root exudates consisted of acetic, butyric, glutaric, lactic, maleic, propionic, pyruvic, succinic, tartaric, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heavy metals; Soil extractant; Organic acids; Rhizosphere; Root exudates.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400039
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Metal-Resistant Rhizobacteria Change Soluble-Exchangeable Fraction in Multi-Metal-Contaminated Soil Samples Rev. Bras. Ciênc. Solo
Boechat,Cácio Luiz; Quadros,Patricia Dörr de; Giovanella,Patrícia; Brito,Ana Clecia Campos; Carlos,Filipe Selau; Sá,Enilson Luiz Saccol de; Camargo,Flávio Anastácio de Oliveira.
ABSTRACT There is a complex interaction between various components of the soil ecosystem, including microbial biomass and soil chemical contaminants such as heavy metals and radionuclides, which may greatly affect the efficiency of bioremediation techniques. The aim of this study was to investigate microbial capacity to change pH, changes in the metal soluble-exchangeable fraction, and effects of initial heavy metal contents on soil samples in microbial solubilization/immobilization capacity. The soil samples used in this study were collected at a known metal-contaminated site. Three highly metal-resistant bacteria were isolated from rhizosphere soil samples collected on weed species identified as Senecio brasiliensis, Senecio leptolobus, and Baccharis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trace elements; Bioremediation technology; Rhizosphere; Environmental contamination.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100514
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Interaction between Thermotolerant Coliforms and Rhizobacteria in Soil Fertilized with Treated Domestic Wastewater Rev. Bras. Ciênc. Solo
Fortes Neto,Paulo; Fortes,Nara Lúcia Perondi; Silva,Eliana Maria de Araújo Mariano da; Brambatti,Fabiana.
ABSTRACT Studies on the survival of pathogenic microorganisms in the soil after use of wastewater for fertilization of agricultural crops report the effects of moisture, pH, organic matter, and soil temperature on microorganisms. There are few studies that assess the survival of these microorganisms in the rhizosphere of plants fertilized with wastewater. Thus, the aim of this study was to quantify the number of fecal coliforms and rhizobacteria (fluorescent Pseudomonas spp., Bacillus spp,) in the rhizosphere of winter and summer crops fertilized with wastewater. In the experiment, we used 20 plots, and each plot occupied an area of 200 m2. The treatments used in the winter crop consisted of uncultivated plots and single crops of wheat, triticale, black...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Reclaimed water; Wastewater.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100414
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Soil nitrogen dynamics after Brachiaria desiccation Rev. Bras. Ciênc. Solo
Castoldi,Gustavo; Reis,Jhônatas Gomes dos; Pivetta,Laércio Augusto; Rosolem,Ciro Antonio.
Brachiaria species, particularly B. humidicola, can synthesize and release compounds from their roots that inhibit nitrification, which can lead to changes in soil nitrogen (N) dynamics, mainly in N-poor soils. This may be important in crop-livestock integration systems, where brachiarias are grown together with or in rotation with grain crops. The objective of the present study was to determine whether this holds true in N-rich environments and if other Brachiaria species have the same effect. The soil N dynamics were evaluated after the desiccation of the species B. brizantha, B. decumbens, B. humidicola, and B. ruziziensis, which are widely cultivated in Brazil. The plants were grown in pots with a dystroferric Red Latosol in a greenhouse. Sixty days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria brizantha; Brachiaria decumbens; Brachiaria humidicola; Brachiaria ruziziensis; Nitrification; Rhizosphere.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000600018
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Soil-Root-Microbe Interactions in the Rhizosphere: A Key to Understanding and Predicting Nutrient Bio availability to Plants R.C. Suelo Nutr. Veg.
Hinsinger,P; Bravin,M.N; Devau,N; Gerard,F; Le Cadre,E; Jaillard,B.
Tipo: Journal article Palavras-chave: Rhizosphere; Soil-root-microbe interactions; Nutrient bioavailability.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400008
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Soil Physicochemical and Biological Interfacial Interactions in the Rhizosphere: Impacts on Food Security and Ecosystem Integrity R.C. Suelo Nutr. Veg.
Huang,P.M.
Tipo: Journal article Palavras-chave: Rhizosphere; Climate change; Nanoparticle.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400005
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Exploring interactions of plant microbiomes Scientia Agricola
Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir.
A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013
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Plant proton pumps as markers of biostimulant action Scientia Agricola
Zandonadi,Daniel Basílio; Santos,Mirella Pupo; Caixeta,Lisanne Santos; Marinho,Eduardo Barros; Peres,Lázaro Eustáquio Pereira; Façanha,Arnoldo Rocha.
ABSTRACT A standard protocol to evaluate the effects of biostimulants on plant physiology is still lacking. The proton pumps present in the vacuolar and plasma membranes are the primary agents responsible for the regulation of the electrochemical gradient that energizes the nutrient uptake system and acid growth mechanism of plant cells. In this study, two of these enzymes were characterized as biochemical markers of biostimulant activity. A simple and fast protocol based on the degree of root acidification using a pH sensitive dye and the Micro-Tom tomato as a plant model is proposed as an efficient methodology to prove the efficacy of biostimulants that are claimed to improve nutrient acquisition and root growth. The results agree with the data from more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micro-Tom; Acidification; Auxin; Rhizosphere; Bromocresol.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000100024
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Soil microbial and chemical properties influenced by continuous cropping of banana Scientia Agricola
Sun,Jianbo; Zou,Liangping; Li,Wenbin; Wang,Yuguang; Xia,Qiyu; Peng,Ming.
ABSTRACT: The impacts of continuous cropping of banana on soil microbiological and biochemical properties are little understood. In this study, we evaluated the variations in soil bacterial community abundance and diversity, microbial biomass carbon (MBC) and nitrogen (MBN) as well as soil enzyme activities involved in C, N and P cycles as affected by continuous cropping of banana. An initial increase in bacterial 16S rRNA copy and soil microbial biomass was observed in the second cropping and then decreased until the fourth cropping. The diversity of bacterial community showed a continuous decrease throughout the experiment. In addition, continuous cropping of banana caused shifts in bacterial community composition and structures. Soil urease and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Soil bacterial abundance; Bacterial community diversity; Soil microbial biomass; Soil enzymes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000500420
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Occurrence of diazotrophic bacteria in Araucaria angustifolia Scientia Agricola
Neroni,Rafaela de Fátima; Cardoso,Elke Jurandy Bran Nogueira.
Araucaria angustifolia is an environmentally threatened tree and the whole biota of the Araucaria Forest should be investigated with the aim of its preservation. Diazotrophic bacteria are extremely important for the maintenance of ecosystems, but they have never been studied in Araucaria Forests. In this study, diazotrophic bacteria were isolated from Araucaria roots and soil, when grown in semi-specific, semi-solid media. The diazotrophic character of some recovered isolates could be confirmed using the acetylene reduction assay. According to their 16S rRNA sequences, most of these isolates belong to the genus Burkholderia.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Biological nitrogen fixation; Endophytic bacteria; Rhizosphere; Forest.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000300015
Registros recuperados: 35
Primeira ... 12 ... Última
 

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