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Última Plant- and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: a review 42 Arcand,Melissa M.; Schneider,Kim D.. Deficiency in plant-available phosphorus is considered to be a major limiting factor to food production in many agricultural soils. Mineral resources are necessary to restore soil phosphorus content. In regions where conventional fertilizers are not used due to cost limitations or to mitigate adverse environmental effects, local sources of phosphate rock are being increasingly recognized for potential use as alternative phosphorus fertilizers. The main obstacle associated with using directly applied ground phosphate rock is that the phosphate released is often unable to supply sufficient plant-available phosphorus for crop uptake. Plantand microbial-based mechanisms are low-cost, appropriate technologies to enhance the solubilization and increase the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agriculture; Microorganisms; Phosphate rock; Phosphorus; Rhizosphere; Solubilization. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400013 Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi 42 Souchie,Edson L.; Azcón,Rosario; Barea,Jose M.; Silva,Eliane M.R.; Saggin-Júnior,Orivaldo J.. This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger... Tipo: Info:eu-repo/semantics/article Palavras-chave: Araxá apatite; Phosphate solubilization; Co-inoculation; Plant growth promotion; Rhizosphere. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300023 Aspergillus niveus Blochwitz 4128URM: new source for inulinase production 52 Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de. Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003 Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes 52 Bumunang,Emmanuel Wihkochombom; Babalola,Olubukola Oluranti. This study was done to examine the rhizobacteria from field grown Genetically Modified (GM) maize and its non-GM counterpart. Rhizospheric soil samples were collected at 30 days after sowing (DAS) and at post-harvest from two experimental fields in Gauteng, South Africa. Total rhizobacteria (cfu/g) in GM and non-GM soil samples was not significantly different across the different media 30 DAS and at post-harvest. Rhizobacterial isolates obtained were biochemically characterized using the analytical profile index. Species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Stenotrophomonas, Achromobacter, Ewingella and Bacillus were screened in vitro for plant growth promoting traits such as, ammonia production, catalase activity, indole acetic acid... Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetically modified maize; Plant growth promoting traits; Rhizosphere; Rhizobacteria. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100001 Rhizosphere Bacterial Composition of the Sugar Beet Using SDS-PAGE Methodology 52 Aeini,Milad; Khodakaramian,Gholam. ABSTRACT The rhizosphere zone has been defined as the volume of soil directly influenced by the presence of living plant roots or soil compartment influenced by the root. During the growing season of 2014, the rhizobacteria of 23 sugar beet plants sampled from 12 sites in the west and north west of Iran were inventoried. Using a cultivation-dependent approach, a total of 217 bacteria were isolated from the rhizosphere. The bacterial isolates were tentatively grouped and documented based on polyacrylamide gel electrophoresis of whole-cell proteins and were found to represent 43 different protein electrotypes. The majority of the fingerprint types were found only on a single occasion. Fifty-nine percent of the strains belonged to the five bacterial species... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Sugar beet; SDS-PAGE; Protein fingerprint. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100451 Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate 58 Tahir,Muhammad; Khalid,Umaira; Ijaz,Muhammad; Shah,Ghulam Mustafa; Naeem,Muhammad Asif; Shahid,Muhammad; Mahmood,Khalid; Ahmad,Naveed; Kareem,Fazal. Abstract This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150–100 NP kg ha−1) and half dose (75–50 NP kg ha−1) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments. The cultivar Punjab-2011 produced the higher chlorophyll contents, crop growth rate, and the straw yield at half dose of NP fertilizer; while... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphorous; Phosphorous solubilizing bacteria; Bioavailability; Rhizosphere; Low fertilizer input. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500015 Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing 58 Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad. In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031 Assessing the diversity of bacterial communities associated with plants 58 Andreote,Fernando Dini; Azevedo,João Lúcio; Araújo,Welington Luiz. Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant-bacteria interactions; Molecular techniques; Multivariate analysis; Endophytes; Rhizosphere. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300001 Fluorescent Pseudomonads associated with the rhizosphere of crops: an overview 58 Botelho,Gloria R.; Mendonça-Hagler,Leda Cristina. Environmental concerns have led to the need of sustainable use of natural resources. The conventional agriculture caused considerable impacts on soils and waters. It is important to change certain agricultural managements to environmental cleaner techniques. The sustainable agriculture has pointed many approaches and techniques to reduce environmental impact. One of those strategies is the utilization of the soil microbiota to induce plant growth, control of plant diseases and biodegradation of xenobiotic compounds. Studies on the relationship between roots and microbiota are essential to achieve viable agricultural applications. These studies indicate that one of the most abundant microorganisms in the rhizosphere (area around the roots) is fluorescent... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting; Biological control; Fluorescent Pseudomonads; Rhizosphere. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400001 Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin 58 Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Nascimento,João Paulo; Laranjeira,Delson. Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Fungi were evaluated according to their capacity to hydrolyse inulin and the variation in the diversity of these fungi during the plant's life cycle was also accessed. Forty-nine species of filamentous fungi were isolated. Penicillium and Aspergillus were the genera that presented higher number of species, nine and seven, respectively. At the end of the sunflower life cycle, cultivated both in field and in the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Rhizosphere; Soil; Inulinase; Sunflower. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300018 Trichoderma harzianum MTCC 5179 impacts the population and functional dynamics of microbial community in the rhizosphere of black pepper (Piper nigrum L.) 58 Umadevi,Palaniyandi; Anandaraj,Muthuswamy; Srivastav,Vivek; Benjamin,Sailas. Abstract Employing Illumina Hiseq whole genome metagenome sequencing approach, we studied the impact of Trichoderma harzianum on altering the microbial community and its functional dynamics in the rhizhosphere soil of black pepper (Piper nigrum L.). The metagenomic datasets from the rhizosphere with (treatment) and without (control) T. harzianum inoculation were annotated using dual approach, i.e., stand alone and MG-RAST. The probiotic application of T. harzianum in the rhizhosphere soil of black pepper impacted the population dynamics of rhizosphere bacteria, archae, eukaryote as reflected through the selective recruitment of bacteria [Acidobacteriaceae bacterium (p = 1.24e-12), Candidatus koribacter versatilis (p = 2.66e-10)] and fungi [(Fusarium... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Population abundance; Functional abundance. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300463 An optmized protocol for simultaneous extraction of DNA and RNA from soils 58 Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia. In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order... Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011 Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile 118 Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S... Tipo: Journal article Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018 Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis 69 Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu. A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize... Tipo: Journal article Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007 Plant growth-promoting bacteria as inoculants in agricultural soils 74 Souza,Rocheli de; Ambrosini,Adriana; Passaglia,Luciane M.P.. Abstract Plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. Plant growth-promoting bacteria (PGPB) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, ACC deaminase activity, and production of siderophores and phytohormones, can be assessed as plant growth promotion (PGP) traits. Bacterial inoculants can contribute to increase agronomic efficiency by reducing production costs... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen fixation; Siderophore production; Plant-bacteria interaction; Inoculant; Rhizosphere. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400401 Ocorrência de nematóides na rizosfera de banana (Musa spp.) e abacaxi (Ananas comosus L. Merril) em Rio Branco, AC. 14 CAVALCANTE, M. de J. B.; SHARMA, R. D.; GONDIM, T. M. de S.. O presente trabalho teve como objetivo verificar a presença de nematóides em bananeiras e abacaxizeiros, cultivados no Campo Experimental da Embrapa Acre, que se apresentavam com início de declínio. Tipo: Folhetos Palavras-chave: Piñas; Amazonia Occidental; Granjas de demostración; Nematodo de la raíz; Embrapa Acre; Rio Branco (AC); Acre; Amazônia Ocidental; Western Amazon; Abacaxi; Ananás Comosus; Banana; Plátano; Rizosfera; Campo Experimental; Musa sp; Nematóide; Pineapples; Demonstration farms; Root-knot nematodes; Rhizosphere. Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/500845 Disponibilização de K de rochas silicáticas para plantas de soja inoculadas com fungos micorrízicos arbusculares (FMA). 14 ANDRADE, L. R. M. de; FALEIRO, A. S. G.; SANTOS SOBRINHO, D. A.; NASCIMENTO, M. T. DO; SOUZA, A. L. DE. Tipo: Folhetos Palavras-chave: Fungo micorrízico arbuscular; Nutrientes; Arbuscular mycorrhizal fungus; Rizosfera; Soja; Nutrients; Rhizosphere; Soybeans. Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/931541 Métodos quantitativos para determinação de características bioquímicas e fisiológicas associadas com bactérias promotoras do crescimento vegetal. 14 CATTELAN, A. J.. Metodologia para as análises em laboratório; Produção de sideróforos; Produção de ácido indol acético (AIA); Produção de citocininas e giberelinas; Fixação assimbiotica de N2; Produção de quitinase; Producao de B-1,3-glucanase; Produção de 1-aminociclopropano-1-carboxylato deaminase; Produção de ácido cianidrico; Solubilizacao de fosfatos; Produção de pectinase; Produção de celulase; Antagonismo direto a fungos; Antagonismo indireto a fungos (compostos volateis); Antagonismo entre bacterias. Tipo: Folhetos Palavras-chave: Rizobacteria; Crescimento de planta; Fisiologia de planta; Plant; Growth; Bactéria; Fisiologia Vegetal; Inoculação; Rhizobium; Brazil; Inoculation methods; Plant physiology; Rhizosphere. Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/461542 Ocorrência de nematóides na rizosfera de pimenta longa (Piper hispidinervum). 14 CAVALCANTE, M. de J. B.; SHARMA, R. D.. Esta publicação relata o ocorrência de Meloidogyne javanica nos cultivos de pimenta longa. Tipo: Folhetos Palavras-chave: Nematodo de la raíz; Pimenta Longa; Piper Hispidinervum; Helminto; Nematóide; Meloidogyne Javanica; Meloidoginose; Rizosfera; Root-knot nematodes; Piper longum; Rhizosphere; Helminths. Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/500847 Effect of plant photosynthesis, carbon sources and ammonium availability on nitrogen fixation rates in the rhizosphere of Zostera noltii 5 Welsh, Dt; Bourgues, S; Dewit, R; Auby, Isabelle. Rates of nitrogen fixation (measured as acetylene reduction) in the rhizosphere of the seagrass Zostera noltii were highly dependent upon plant photosynthetic activity being significantly stimulated at elevated CO2 concentrations and by light, both in the short-term and over diurnal cycles. Stimulation by light became insignificant when 5 mM sucrose was added to the sediment porewater, indicating that in the absence of added carbon sources, light stimulation was due to direct inputs of plant photosynthate to the rhizosphere. Addition of a range of carbon sources to the rhizosphere sediment stimulated rates of acetylene reduction, with this stimulation being significant for sucrose and lactate. Surprisingly, whilst low additions of ammonium to the sediment... Tipo: Text Palavras-chave: Acetylene reduction; Nitrogen fixation; Rhizosphere; Zostera noltii; Plant photosynthesis; Root exudates; Carbon sources; Ammonium. Ano: 1997 URL: http://archimer.ifremer.fr/doc/00151/26266/24350.pdf Registros recuperados: 36 Primeira ... 1 2 ... Última

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