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Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability ArchiMer
Peruzzi, Stefano; Chatain, Beatrice.
The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1 degrees C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm(-2)) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was...
Tipo: Text Palavras-chave: Parental variability; Pressure and cold shocks; Dicentrarchus labrax; Sea bass; Triploidy; Meiotic gynogenesis.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-507.pdf
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Genetic investigation of swimbladder inflation anomalies in the European sea bass, Dicentrarchus labrax L. ArchiMer
Peruzzi, Stefano; Westgaard, Jon-ivar; Chatain, Beatrice.
Although the actiology of swimbladder inflation anomalies in important aquaculture species such as European sea bass D. labrax is not fully determined, culture conditions are commonly suggested as main contributory factors. Little information is available on whether swimbladder inflation has a genetic basis for its expression too. In this work, 24 full-sibling sea bass families from a 4 dams x 6 sires factorial crossing were reared under communal conditions. The larvae developing normal and abnormal (uninflated or hyper-inflated) swimbladders were genotyped at four microsatellite loci, Labrax-3, Labrax-13, Labrax-17, Labrax29, and allocated to the individual breeders. Out of 273 offspring, 97% could be assigned to a single parental pair. The genotype and...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Genetics; Anomalies; Swimbladder.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2590.pdf
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Dietary TAG source and level affect performance and lipase expression in larval sea bass (Dicentrarchus labrax) ArchiMer
Morais, Sophia; Cahu, Chantal; Zambonino-infante, Jose-luis; Robin, Jean; Ronnestad, I; Dinis, M; Conceicao, L.
The influence of dietary TAG source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mRNA level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. Fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. Coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain TAG. Growth was not related to lipase specific activity, suggesting a production in excess to dietary needs. Body lipid content was positively related to dietary lipid level and was affected by lipid quality. In addition, larval FA composition generally reflected that of the diet. The...
Tipo: Text Palavras-chave: Lipase activity; Dietary; Growth; Larvae; Sea bass; Fish culture.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-785.pdf
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L'élevage du Loup tropical (Lates calcarifer, Bloch) en Polynésie Française. Approche technico-économique ArchiMer
Pierson, R; Thouard, Emmanuel; Nedelec, Georges.
Due to lack of knowledge about the seabass (Lates calcarifer) market in Polynesia, various production levels were considered to develop a managing tool for decision, making relevant development strategy and adapted rearing technology. This work is an analysis of the economical parameters of known rearing techniques depending on various production levels. The comparative study of the techniques, phase by phase, regarding the production cost and the investments prompted us to select the semi-intensive larval rearing technology under the current economical conditions. However, the larval rearing extensive technique could be of highest interest for productions lower than 60 metric tons a year. Growing-out in floating net-cages appeared to be the only...
Tipo: Text Palavras-chave: Aquaculture; Loup tropical; Lates calcarifer; Coût de production; Aquaculture; Sea bass; Lates calcarifer; Production cost.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00309/41998/41280.pdf
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Population, Temperature and Feeding Rate Effects on Individual Feed Efficiency in European Sea Bass (Dicentrarchus labrax) ArchiMer
Rodde, Charles; Vandeputte, Marc; Allal, Francois; Besson, Mathieu; Clota, Frederic; Vergnet, Alain; Benzie, John A. H.; De Verdal, Hugues.
Using breeding programs to improve feed efficiency, the ratio between fish body weight gain (BWG) and feed intake (FI), could increase aquaculture sustainability through reduced feed costs and environmental impact. To this end, individual phenotypic information is required. Individual FI can be measured by isolating each fish. Under these conditions, restricting the feeding rate has proved relevant to improve feed efficiency indirectly by selecting faster-growing animals. Moreover, a restricted feeding rate reduces the work load of collecting uneaten pellets after each meal. The approach assumes the most efficient fish at high and low feeding rates are the same, but this assumption remains untested. In European sea bass (Dicentrarchus labrax), feed...
Tipo: Text Palavras-chave: Aquaculture; Individual rearing; Feed efficiency; Feeding rate; Fasting tolerance; Sea bass.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00654/76614/77767.pdf
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Effect of chronic exposure to ammonia on growth, food utilisation and metabolism of the European sea bass (Dicentrarchus labrax) ArchiMer
Dosdat, Antoine; Person, Jeannine; Coves, Denis; Dutto, Gilbert; Gasset, Eric; Le Roux, Annick; Lemarie, Gilles.
The chronic effects of exposing sea bass (average initial weight 100 g) to ammonia in water at 22 degreesC were first evaluated over a 61-day period (period 1, P1) during which nine different groups were submitted to nine ambient ammonia levels ranging from 0.014 to 0.493 mg 1(-1) NH3-N (0.53-16.11 mg 1(-1) total ammonia nitrogen (TA-N)) and fed using self-feeders. At the end of P1, the fish were starved for 10 days (P2). Their recovery capacity was tested over 43 days (P3) after which the exogenous ammonia supply was stopped in all treatments and the fish were allowed to feed. After 20 days of exposure a highly significant effect of ammonia was evident from the decrease in feeding activity, voluntary feed intake (VFI) and specific growth rate (SGR), and...
Tipo: Text Palavras-chave: Sea bass; Self feeding; Compensatory growth; Chronic toxicity; Ammonia.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-595.pdf
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Long-term monitoring of individual fish triggering activity on a self-feeding system: An example using European sea bass (Dicentrarchus labrax) ArchiMer
Coves, Denis; Beauchaud, M; Attia, J; Dutto, Gilbert; Bouchut, C; Begout, Marie-laure.
In two experiments, a computerized on-demand feeding system coupled with a PIT tag monitoring device was used to continuously record the triggering activity by ca. 50 individual sea bass for 55 days (Exp. 1, initial average body weight and coefficient of variation, CV: 299 g, 15%) and 69 days (Exp. 2, 157 g, 13%). Each group was stocked in 1 m(3) tanks and exposed to a water temperature of 21 +/- 1 degrees C and a light regime of 16:8 LD. Only 5% of the triggering activity was not associated with simultaneous PIT tag detection. Although each individual was detected via PIT tag during the experiment, only 67% and 74% of the fish actuated the trigger at least once in Exp. 1 and Exp. 2, respectively. Moreover, only two fish in Exp. 1 and one fish in Exp. 2...
Tipo: Text Palavras-chave: PIT tag detection antenna; Individual triggering activity; Self feeding; Sea bass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1341.pdf
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Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus ArchiMer
Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf
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Different survival of three populations of European sea bass (Dicentrarchus labrax) following challenge with two variants of nervous necrosis virus (NNV) ArchiMer
Barsøe, Sofie; Allal, Francois; Vergnet, Alain; Vandeputte, Marc; Olesen, Niels Jørgen; Schmidt, Jacob Günther; Larsen, Cathrine Agnete; Cuenca, Argelia; Vendramin, Niccolò.
Viral Nervous Necrosis (VNN, also called viral encephalo- and retinopathy (VER)), is a widespread disease of marine aquaculture caused by betanodavirus (or nervous necrosis virus - NNV), a segmented positive sense RNA virus, member of the nodaviridae family. VNN affects predominantly marine fish and cause significant losses to the Mediterranean fish farming industry, including the production of European sea bass (Dicentrarchus labrax). Of the four circulating genotypes of betanodavirus, red-spotted grouper NNV (RGNNV) and the reassortant genotype red-spotted grouper/striped jack NNV (RG/SJNNV) are most prevalent in the Mediterranean. Inheritable resistance against VNN has been detected in sea bass, and selective breeding could be a mean to limit this...
Tipo: Text Palavras-chave: Nervous necrosis virus; Betanodavirus; Viral enchephalo-and retinopathy; Genetic resistance; Host-pathogen interaction; Sea bass; Neurological infection.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00678/79050/81498.pdf
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Contribution of dietary arginine to nitrogen utilisation and excretion in juvenile sea bass (Dicentrarchus labrax) fed diets differing in protein source ArchiMer
Tulli, F; Vachot, Christianne; Tibaldi, E; Fournier, Vincent; Kaushik, Sadasivam.
The role of dietary arginine in affecting nitrogen utilisation and excretion was studied in juvenile European sea bass (Dicentrarchus labrax) fed for 72 days with diets differing in protein sources (plant protein-based (PM) and fish-meal-based (FM)). Fish growth performance and nitrogen utilisation revealed that dietary Arg surplus was beneficial only in PM diets. Dietary Arg level significantly affected postprandial plasma urea concentrations. Hepatic arginase activity increased (P < 0.05) in response to dietary Arg surplus in fish fed plant protein diets; conversely ornithine transcarbamylase activity was very low and inversely related to arginine intake. No hepatic carbamoyl phosphate synthetase III activity was detected. Dietary arginine levels did...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Excretion; Arginase; Urea; Arginine.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-6317.pdf
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Influence of tank volume on vitellogenesis and spawning performances in sea bass Dicentrarchus labrax L. ArchiMer
Buchet, Vincent; Coquard, Elodie; Severe, Armelle; Barone, Herve.
Sea bass, Dicentrarchus labrax (mean weight: 748 +/- 13 g), were maintained before and during vitellogenesis in 1, 3, 8, 16 and 32 m(3) tanks, and then they were transferred to 2 m(3) tanks, for the spawning season. During the first 2 months of the experiment, the growth rates were significantly lower in smaller tanks (1 m(3)). In August, the oocyte diameters were significantly lower in smaller tanks (1, 3 and 8 m(3)) than in larger (16-32 m(3)) tanks. At the end of the experiment, the fish mean weight in the 1 m(3) tanks was significantly lower than in the 3 m(3) tanks, but oocyte diameters and plasma oestradiol concentrations were not significantly different between the volumes. This shows a longer acclimation requirement in smaller volume rearing....
Tipo: Text Palavras-chave: Volume; Aquaculture; Vitellogenesis; Reproduction; Sea bass.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4068.pdf
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La pisciculture marine : un secteur industriel en émergence ArchiMer
Paquotte, Philippe; Harache, Yves.
In a general context of low progression, European aquaculture has been characterized by the development of intensive fish farming. The most notable fact is the recent increase in intensive salmonid farming, with a turnover of almost two million ECU. More recently, the intensive farming of sea bass and sea bream has increased very rapidly in the Mediterranean Basin, although on a more reduced extent. Although research has started as soon as the seventies with a strong public incentive both for salmon, sea bass and sea bream, the development of these different types of aquaculture has not been done at the same pace. Some elements of sectorial dynamics are analysed such as research planning and financing, organization of the production and public policy. For...
Tipo: Text Palavras-chave: Europe; Environmental protection; Economic development; Production structure markets; Saltwater fishes; Sea bream; Sea bass; Atlantic salmon; Marine environment; Fish culture; SEM; Europe; Protection de l'environnement; Développement économique; Marché; Structure de production; Poisson de mer; Dorade; Bar; Saumon atlantique; Milieu marin; Pisciculture.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-4104.pdf
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Cardiac preload and venous return in swimming sea bass (Dicentrarchus labrax L.) ArchiMer
Sandblom, Erik; Farrell, Anthony; Altimiras, Jordi; Axelsson, Michael; Claireaux, Guy.
Cardiac preload (central venous; pressure, Pcv), mean circulatory filling pressure (MCFP), dorsal aortic blood pressure (P-DA) and relative cardiac output (Q) were measured in sea bass (Dicentrarchus labrax) at rest and while swimming at 1 and 2 BL s(-1). MCFP, an index of venous capacitance and the upstream venous pressure driving the return of venous blood to the heart, was measured as the plateau in Pcv during ventral aortic occlusion. Compared with resting values, swimming at 1 and 2BL s(-1) increased Q (by 15 +/- 1.5 and 38 +/- 6.5%, respectively), Pcv (from 0.11 +/- 0.01 kPa to 0.12 +/- 0.01 and 0.16 +/- 0.02 kPa, respectively), MCFP (from 0.27 +/- 0.02 kPa to 0.31 +/- 0.02 and 0.40 +/- 0.04 kPa, respectively) and the calculated pressure gradient for...
Tipo: Text Palavras-chave: Blood pressure; Cardiac rythm; Swimming aquaculture; Sea bass.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-421.pdf
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Effects of temperature, stocking density and farming conditions on fin damage in European sea bass (Dicentrarchus labrax) ArchiMer
Person, Jeannine; Le Bayon, Nicolas.
This paper presents a non invasive, rapid and reliable way to quantitatively assess fin erosion in sea bass (Dicentrarchus labrax). The method is based on a visual assessment of fin profile and area loss of all fins except the anterior dorsal, on a scale from 0 to 4 in comparison with a perfect fin. The effects of stocking density (SD) and temperature on fin damage were investigated under experimental conditions (100-250 g fish). Over a 4-month period, mean erosion index (mean erosion level of all fins) was 10 times higher at 120 than at 20 kg m(-3), where most fins were undamaged. Damage was also dependent on time and oxygen concentration (35% lower at 53% O-2 saturation than at 105%). Fin condition was also affected by temperature: mean erosion index was...
Tipo: Text Palavras-chave: Rearing systems; Stocking density; Temperature; Welfare; Fin damage; Sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6873.pdf
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Comparative effects of long-term hypoxia on growth, feeding and oxygen consumption in juvenile turbot and European sea bass ArchiMer
Pichavant, Karine; Person, Jeannine; Le Bayon, Nicolas; Severe, Armelle; Le Roux, Annick; Boeuf, Gilles.
When juvenile turbot Scophthulmus maximus and sea bass Dicentrarchus labrax were fed to satiation, growth and food intake were depressed under hypoxia (3.2 +/- 0.3 and 4.5 +/- 0.2 mg O-2 l(-1)). However, no significant difference in growth was observed between fishes maintained in hypoxia and fed to satiation and fishes reared in normoxia (7.4 +/- 0.3 mg O-2 l(-1)) and fed restricted rations (same food intake of fishes at 3.2 mg O-2 l(-1)). Routine oxygen consumption of fishes fed to satiation was higher in normoxia than in hypoxia due to the decrease in food intake in the latter. Of the physiological parameters measured, no significant changes were observed in the two species maintained in hypoxia. This study confirms the significant interaction between...
Tipo: Text Palavras-chave: Oxygen consumption; Growth; Hypoxia; Sea bass; Turbot.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-617.pdf
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Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis ArchiMer
Darias, Maria J.; Zambonino, Jose-luis; Hugot, K; Cahu, Chantal; Mazurais, David.
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were...
Tipo: Text Palavras-chave: Sea bass; Gene expression; Microarray; Development; Fish larvae.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4344.pdf
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Fish growth and health aspects of sea bass (Dicentrarchus labrax) reared in standard vs. high rate algal pond recirculation systems ArchiMer
Mladineo, Ivona; Bocina, Ivana; Przybyla, Cyrille; Fievet, Julie; Blancheton, Jean-paul.
Recirculation aquaculture system (RAS) is the type of land-based aquaculture production that interferes the least with the environment, particularly due to low effluent volume and overall water requirement. Water reuse, sometimes limited by the accumulation of waste products excreted by fish, can be improved by incorporating another biotechnical component in the form of high rate algal pond (RAS+HRAP), thus adding to the economy and self sustainability of the system. Our goal was to evaluate whether rearing fish for 1 year in a RAS or RAS+HRAP led to differences in fish production and health, by assessing: (1) fish growth; (2) impact on the morphological structure of sea bass target tissues (liver, kidney, and spleen); (3) susceptibility to monogenean...
Tipo: Text Palavras-chave: Health; High rate algal pond; Recirculation; Sea bass.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12228/9010.pdf
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Is total serum protein a good indicator for welfare in reared sea bass (Dicentrarchus labrax)? ArchiMer
Coeurdacier, Jean-luc; Dutto, Gilbert; Gasset, Eric; Blancheton, Jean-paul.
Reared sea bass were submitted to six stressful situations: hyperoxia with or without hypercapnia, increased stocking density in an open flow or recirculating system, transfer to another tank and nodavirus injection. The potentially negative impact of these factors on the lives of sea bass was investigated by measuring 9 water parameters and 19 fish parameters including total serum protein (TSP). TSP has already been used to evaluate stress. The present study investigates data of previous and new experiments, concentrating on the potential use of TSP as a routine indicator to assess welfare in sea bass reared on fish farms. In the current experiment, oxygen and carbon dioxide were seen to affect levels of TSP, but alterations were too erratic to enable...
Tipo: Text Palavras-chave: Fish stress; Fish welfare; Protein; Serum; Fish transfer; Nodavirus; Sea bass.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15382/12740.pdf
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Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. ArchiMer
Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert.
The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf
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Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes ArchiMer
Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P.
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage...
Tipo: Text Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf
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