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Disector Z-axis mechanical method for stereology Anais da ABC (AABC)
Xavier-Vidal,Ricardo.
The purpose of this work is to calibrate a not expensive microscope to be applied in Optical Disector estimation. The evaluation of "Z-axis" bias and the "Z-axis" calibration were made utilizing a cover slip and a "manual digimatic outside micrometer scale" (Mitutoyo, Japan). Calibrating the cover slip we performed the calibration of the "Z-axis" of a microscope. In the cover slip two lines were painted with different colors using a pen glass. A blue line was painted on the up surface and another line (red) on the bottom surface of the cover slip forming a cross. Two metal rings with one palette welded in each were adapted in the microscope. Other palette was welded in the gross focuses in order to restrict the route of the fine focuses (Z-axis study)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stereology; Disector; Optical disector; Morphometry; Z-axis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200028
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Stereological tools in biomedical research Anais da ABC (AABC)
Mandarim-de-Lacerda,Carlos A..
Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Stereology; Volume density; Surface density; Length density; Disector; Fractionator.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000400006
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The impact of long-term consumption of diets enriched with olive, cottonseed or sesame oils on kidney morphology: A stereological study Anais da ABC (AABC)
HASSANZADEH-TAHERI,MOHAMMADMEHDI; HASSANZADEH-TAHERI,MAHSA; JAHANI,FARNAZ; ERFANIAN,ZAHRA; MOODI,HESAM; HOSSEINI,MEHRAN.
Abstract: To date, most of studies have only focused on metabolic effects of dietary oils while recent evidence proposes that they can influence kidneys structure. Therefore, the aim of this study was to evaluate the impact of long-term consumption of olive, cottonseed and sesame oils on renal morphology in rats. 70 male Wistar rats randomly assigned into seven equal groups and treated with standard diet (control), the standard diet enriched with 10% or 20% (W/W) of either olive oil (OLI10%, OLI20%), cottonseed oil (COT10%, COT20%) or sesame oil (SES10%, SES20%) for 5 months. Quantitative features of the kidney including kidney and cortex volumes and the number of glomeruli were analyzed stereologically. Moreover, kidney sections histologically were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cottonseed oil; Histopathology; Kidney; Olive oil; Sesame oil; Stereology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300702
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The quantification of testicular cells during the postnatal development in two Caviomorph rodents: the guinea pig (Cavia porcellus) and the cutia (Dasyprocta agouti) Anais da ABC (AABC)
SIMÕES,LUCIANA S.; SASAHARA,TAIS H.C.; FAVARON,PHELIPE O.; MARQUES JÚNIOR,RICARDO; OLIVEIRA,MOACIR F. DE; MACHADO,MÁRCIA R.F.; MIGLINO,MARIA ANGELICA.
ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Germinative cells; Hystricomorpha; Reproduction; Stereology.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401745
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Long lasting structural changes in primary motor cortex after motor skill learning: a behavioural and stereological study Biol. Res.
MORALES,PAOLA.
Many motor skills, once acquired, are stored over a long time period, probably sustained by permanent neuronal changes. Thus, in this paper we have investigated with quantitative stereology the generation and persistence of neuronal density changes in primary motor cortex (MI) following motor skill learning (skilled reaching task). Rats were trained a lateralised reaching task during an "early" (22-31 days oíd) or "late" (362-371 days oíd) postnatal period. The trained and corresponding control rats were sacrificed at day 372, immediately after the behavioural testing. The "early" trained group preserved the learned skilled reaching task when tested at day 372, without requiring any additional training. The "late" trained group showed a similar capacity to...
Tipo: Journal article Palavras-chave: Motor cortex; Plasticity; Persistence of structural changes; Aging; Motor skill learning (skilled reaching task); Stereology; Rat.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400005
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Testicular morphology of adult wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion BABT
Monteiro,Juliana Castro; Matta,Sérgio Luis Pinto da; Predes,Fabrícia Souza; Paula,Tarcízio Antônio Rego de.
The present study was carried out to investigate the effects of Rudgea viburnoides infusion on the body biometry and testicular morphometry and stereology of adult Wistar rats. Two groups received the infusion daily at the concentration of 3 or 6 mg/mL for 40 days. The control group received only water. Neither the biometrical parameters nor the tubular diameter and the height of the seminiferous epithelium showed any significant alterations in the treated animals. Leydig cells stereology did not show any significant alterations in the treated animals. These results indicated that R. viburnoides did not cause alterations in body biometry and testicular morphometry.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Congonha-de-bugre; Morphometry; Stereology; Leydig cell.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100013
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Early effects of estrogen on the rat ventral prostate BJMBR
García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F..
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002
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Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study BJMBR
Abreu,C.C.; Fernandes,T.N.; Henrique,E.P.; Pereira,P.D.C.; Marques,S.B.; Herdeiro,S.L.S.; Oliveira,F.R.R.; Magalhães,N.G.M.; Anthony,D.C.; Melo,M.A.D.; Guerreiro-Diniz,C.; Diniz,D.G.; Picanço-Diniz,C.W..
Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary exercise, whereas the IE had none of the above. A third plus-maze aquarium was used for spatial and object recognition tests. Different visual clues in 2 of its 4 arms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carassius auratus; Spatial learning and memory; Object recognition; Stereology; Telencephalon; Environmental enrichment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500602
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Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice BJMBR
Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A..
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P < 0.0001), and exercise reduced it by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008
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Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet BJMBR
Barros,L.A.; Costa-Silva,M.; Biolchini,C.L.; Neves,R.H.; Machado-Silva,J.R..
A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Schistosoma mansoni; Low-protein diet; Stereology; Praziquantel.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900007
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Microglia and neurons in the hippocampus of migratory sandpipers BJMBR
Diniz,C.G.; Magalhães,N.G.M.; Sousa,A.A.; Santos Filho,C.; Diniz,D.G.; Lima,C.M.; Oliveira,M.A.; Paulo,D.C.; Pereira,P.D.C.; Sherry,D.F.; Picanço-Diniz,C.W..
The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hippocampus; Neurons; Microglia; Shorebirds; Stereology; Morphometry.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100603
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Exendin-4 effects on islet volume and number in mouse pancreas BJPS
Khorsandi,Layasadat; Nejad-Dehbashi,Fereshteh.
The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exendin-4/effects; Exendin-4/experimental study; Pancreas/effects fo Exendin-4; Islet/hypertrophy; Stereology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400014
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Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy ArchiMer
Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M..
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in...
Tipo: Text Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf
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Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae ArchiMer
Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E..
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly...
Tipo: Text Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf
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Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study International Journal of Morphology
Martins,Alex Tadeu; Azoubel,Reinaldo; Lopes,Ruberval A.; di Matteo,Miguel A. Sala; de Arruda,José Germano Ferraz.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision...
Tipo: Journal article Palavras-chave: Sodium cyclamate; Fetal liver; Karyometry; Stereology.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005
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Effects of Preweaning Polysensorial Enrichment upon Development of the Parietal Cortical Plate of Undernourished Rats: A Stereological Study International Journal of Morphology
González,Héctor; Adaro,Luis; Hernández,Alejandro; Fernández,Víctor.
This investigation was undertaken in order to quantify the effects of early polysensorial enrichment on the development of cortical pyramids, located in the parietal cortex of rats simultaneously submitted to protein-energy undernutrition. A short period of stimulation during suckling significantly decreases the cellular density in the cortical plate (phylogenetic-ontogenetic evolutionary index). Results suggest that the cerebral cortex develops according to a sophisticated neuronal network, which exhibits a notable degree of structural specificity, regulated by genetic and environmental clues. The most obvious prediction is that environmental influences are probably transduced as a structural expression in the developing parietal cortical plate.
Tipo: Journal article Palavras-chave: Environmental enrichment; Cortical gradients; Undernutrition; Parietal cortex; Stereology.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400016
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MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL International Journal of Morphology
Cortez,C. M.; Costa,W. S.; Babinski,M. A.; Chagas,M. A..
The present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. The study was based on morphometric and stereological analysis of the perikarya. Eight rats were treated intraperitoneally with cortisol (1mg/Kg/day) during 36 days. Treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. There was a significant difference (<=P0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). The decrease of perikarya neuronal diameter was also demonstrated by stereological methods. Morphometrical findings may suggest alterations in superior cervical...
Tipo: Journal article Palavras-chave: Morphometry; Stereology; Glucocorticoid; Sympathetic ganglion; Rat.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300006
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Morphometric Analysis of Hemicerebellar Asymmetryn with Central Vertigo Cases: A Stereological Study International Journal of Morphology
Nuket Gocmen-Mas,H; Karabekir,Selim; Yilmaz Kusbeci,Ozge; Sahin,Bunyamin; Ertekin,Tolga; Bas,Orhan; Canan Yazici,A; Senan,Sevda.
We aimed to evaluate the relevant methods of stereology to estimate hemicerebellar asymmetry according to sex in both adult right handed vertigo cases and comparing with healthy cases. The study included 14 adult control subjects and 18 patients with vertigo. The volumes of the cerebellar hemispheres were determined by MRI using the point-counting approach of stereological methods. The mean (±SD) of the right cerebellar hemispheres in the patients with vertigo were 52.49±5.42 cm3 in males, 50.11±4.02 cm3 in females. The mean (±SD) of the left cerebellar hemispheres in the patients with vertigo were 53.11±3.70 cm3 in males, 49.73±4.69 cm3 in females. There was not significant quantitative evidence detected in terms of cerebella asymmetry between sagittal...
Tipo: Journal article Palavras-chave: Cavalier principle; Stereology; Hemicerebellar asymmetry; Magnetic resonance imaging; Vertigo.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200048
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End-To-Side Loop Neurorrhaphy: Axonal Comparative Stereological Study International Journal of Morphology
Boer,Nagib Pezati; Viterbo,Fausto; Faleiros,Humberto Regis; Batigalia,Fernando; Boer,Ana Luísa Ricci; Carvalho Jr,Altair Antonio.
Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose of this study was to compare, among rats, stereological results (axons volume density) after end-to-side neurorrhaphy and after end-to-side loop neurorrhaphy. Thirty Wistar rats were used, divided into six groups (five animals per group), consisting of two control groups (for the fibular and tibial nerves), two study groups for the fibular nerve (one with an end-to-side neurorrhaphy, and the other with an end-to-side loop neurorrhaphy) and two study groups for the...
Tipo: Journal article Palavras-chave: Nerve; Axon; Stereology; Neurorrhaphy.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300032
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Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade International Journal of Morphology
Fernandes-Santos,Caroline; Mendonça,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto.
We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse...
Tipo: Journal article Palavras-chave: Losartan; Angiotensin II; L-NAME; Hypertension; Renin-angiotensin system; Stereology; L-NAME.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400003
Registros recuperados: 31
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