|
|
|
Registros recuperados: 31 | |
|
|
Xavier-Vidal,Ricardo. |
The purpose of this work is to calibrate a not expensive microscope to be applied in Optical Disector estimation. The evaluation of "Z-axis" bias and the "Z-axis" calibration were made utilizing a cover slip and a "manual digimatic outside micrometer scale" (Mitutoyo, Japan). Calibrating the cover slip we performed the calibration of the "Z-axis" of a microscope. In the cover slip two lines were painted with different colors using a pen glass. A blue line was painted on the up surface and another line (red) on the bottom surface of the cover slip forming a cross. Two metal rings with one palette welded in each were adapted in the microscope. Other palette was welded in the gross focuses in order to restrict the route of the fine focuses (Z-axis study)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stereology; Disector; Optical disector; Morphometry; Z-axis. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200028 |
| |
|
|
Mandarim-de-Lacerda,Carlos A.. |
Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Morphometry; Stereology; Volume density; Surface density; Length density; Disector; Fractionator. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000400006 |
| |
|
|
HASSANZADEH-TAHERI,MOHAMMADMEHDI; HASSANZADEH-TAHERI,MAHSA; JAHANI,FARNAZ; ERFANIAN,ZAHRA; MOODI,HESAM; HOSSEINI,MEHRAN. |
Abstract: To date, most of studies have only focused on metabolic effects of dietary oils while recent evidence proposes that they can influence kidneys structure. Therefore, the aim of this study was to evaluate the impact of long-term consumption of olive, cottonseed and sesame oils on renal morphology in rats. 70 male Wistar rats randomly assigned into seven equal groups and treated with standard diet (control), the standard diet enriched with 10% or 20% (W/W) of either olive oil (OLI10%, OLI20%), cottonseed oil (COT10%, COT20%) or sesame oil (SES10%, SES20%) for 5 months. Quantitative features of the kidney including kidney and cortex volumes and the number of glomeruli were analyzed stereologically. Moreover, kidney sections histologically were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cottonseed oil; Histopathology; Kidney; Olive oil; Sesame oil; Stereology. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300702 |
| |
|
|
SIMÕES,LUCIANA S.; SASAHARA,TAIS H.C.; FAVARON,PHELIPE O.; MARQUES JÚNIOR,RICARDO; OLIVEIRA,MOACIR F. DE; MACHADO,MÁRCIA R.F.; MIGLINO,MARIA ANGELICA. |
ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germinative cells; Hystricomorpha; Reproduction; Stereology. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401745 |
| |
|
| |
|
| |
|
|
García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F.. |
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002 |
| |
|
|
Abreu,C.C.; Fernandes,T.N.; Henrique,E.P.; Pereira,P.D.C.; Marques,S.B.; Herdeiro,S.L.S.; Oliveira,F.R.R.; Magalhães,N.G.M.; Anthony,D.C.; Melo,M.A.D.; Guerreiro-Diniz,C.; Diniz,D.G.; Picanço-Diniz,C.W.. |
Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary exercise, whereas the IE had none of the above. A third plus-maze aquarium was used for spatial and object recognition tests. Different visual clues in 2 of its 4 arms... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carassius auratus; Spatial learning and memory; Object recognition; Stereology; Telencephalon; Environmental enrichment. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500602 |
| |
|
|
Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A.. |
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P < 0.0001), and exercise reduced it by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008 |
| |
|
| |
|
|
Diniz,C.G.; Magalhães,N.G.M.; Sousa,A.A.; Santos Filho,C.; Diniz,D.G.; Lima,C.M.; Oliveira,M.A.; Paulo,D.C.; Pereira,P.D.C.; Sherry,D.F.; Picanço-Diniz,C.W.. |
The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hippocampus; Neurons; Microglia; Shorebirds; Stereology; Morphometry. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100603 |
| |
|
|
Khorsandi,Layasadat; Nejad-Dehbashi,Fereshteh. |
The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Exendin-4/effects; Exendin-4/experimental study; Pancreas/effects fo Exendin-4; Islet/hypertrophy; Stereology. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400014 |
| |
|
|
Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M.. |
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in... |
Tipo: Text |
Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf |
| |
|
|
Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E.. |
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly... |
Tipo: Text |
Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf |
| |
|
|
Martins,Alex Tadeu; Azoubel,Reinaldo; Lopes,Ruberval A.; di Matteo,Miguel A. Sala; de Arruda,José Germano Ferraz. |
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision... |
Tipo: Journal article |
Palavras-chave: Sodium cyclamate; Fetal liver; Karyometry; Stereology. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005 |
| |
|
| |
|
| |
|
|
Nuket Gocmen-Mas,H; Karabekir,Selim; Yilmaz Kusbeci,Ozge; Sahin,Bunyamin; Ertekin,Tolga; Bas,Orhan; Canan Yazici,A; Senan,Sevda. |
We aimed to evaluate the relevant methods of stereology to estimate hemicerebellar asymmetry according to sex in both adult right handed vertigo cases and comparing with healthy cases. The study included 14 adult control subjects and 18 patients with vertigo. The volumes of the cerebellar hemispheres were determined by MRI using the point-counting approach of stereological methods. The mean (±SD) of the right cerebellar hemispheres in the patients with vertigo were 52.49±5.42 cm3 in males, 50.11±4.02 cm3 in females. The mean (±SD) of the left cerebellar hemispheres in the patients with vertigo were 53.11±3.70 cm3 in males, 49.73±4.69 cm3 in females. There was not significant quantitative evidence detected in terms of cerebella asymmetry between sagittal... |
Tipo: Journal article |
Palavras-chave: Cavalier principle; Stereology; Hemicerebellar asymmetry; Magnetic resonance imaging; Vertigo. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200048 |
| |
|
|
Boer,Nagib Pezati; Viterbo,Fausto; Faleiros,Humberto Regis; Batigalia,Fernando; Boer,Ana Luísa Ricci; Carvalho Jr,Altair Antonio. |
Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose of this study was to compare, among rats, stereological results (axons volume density) after end-to-side neurorrhaphy and after end-to-side loop neurorrhaphy. Thirty Wistar rats were used, divided into six groups (five animals per group), consisting of two control groups (for the fibular and tibial nerves), two study groups for the fibular nerve (one with an end-to-side neurorrhaphy, and the other with an end-to-side loop neurorrhaphy) and two study groups for the... |
Tipo: Journal article |
Palavras-chave: Nerve; Axon; Stereology; Neurorrhaphy. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300032 |
| |
|
|
Fernandes-Santos,Caroline; Mendonça,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto. |
We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse... |
Tipo: Journal article |
Palavras-chave: Losartan; Angiotensin II; L-NAME; Hypertension; Renin-angiotensin system; Stereology; L-NAME. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400003 |
| |
Registros recuperados: 31 | |
|
|
|