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Inhibition of lipid peroxidation and iron (II)-dependent DNA damage by extracts of Pothomorphe peltata (L.) Miq BJMBR
Leaves of Pothomorphe peltata (L.) Miq. (Piperaceae) are used locally as anti-inflammatory, antipyretic, hepatoprotective and diuretic infusions and to treat external ulcers and local infections in several parts of the Peruvian, Bolivian and Brazilian Amazon region. The antioxidant activity of different extracts of P. peltata was studied using the hydroperoxide-initiated chemiluminescence assay in liver homogenates, and the methanolic extract was found to have the highest antioxidant activity, with an IC50 = 4 µg/ml. Aqueous and dichloromethane extracts did not show antioxidant activity. The extracts were further evaluated using the thiobarbituric acid-reactive substances (TBARS) assay. Finally, an assay of DNA sugar damage induced by Fe (II) salt was used...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pothomorphe peltata; Antioxidant activity; Hydroperoxide-initiated chemiluminescence; Thiobarbituric acid-reactive substances; DNA-binding activity; Lipid peroxidation.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100014
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Antioxidant and free radical scavenging effects in extracts of the medicinal herb Achyrocline satureioides (Lam.) DC. ("marcela") BJMBR
Desmarchelier,C.; Coussio,J.; Ciccia,G..
Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010
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Stimulation of antioxidant system and lipid peroxidation by abiotic stresses in leaves of Momordica charantia Braz. J. Plant Physiol.
Agarwal,Sheela; Shaheen,Robina.
The purpose of the present work was to evaluate both the antioxidant response system and oxidative stress in leaves from bitter gourd (Momordica charantia L) subjected to NaCl, UV-B and water stresses at three different stages of plant growth: pre-flowering, flowering and post-flowering. Except for peroxidase (POX), all enzyme activities including superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO), glutathione reductase (GR), as well as concentrations of ascorbate (ASA), hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) exhibited maximum values at the flowering stage under all three stresses. All the enzyme activities, SOD, CAT, POX, PPO, GR and the concentrations of ASA, H2O2 and TBARS were elevated under NaCl...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidative enzymes; Environmental stresses; H2O2; Ion concentration; Thiobarbituric acid-reactive substances.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000200007
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