|
|
|
| Registros recuperados: 39 | |
|
| |
|
| |
|
|
| Lee,Ki-Won; Choi,Gi Jun; Kim,Ki-Yong; Yoon,Sei Hyung; Ji,Hee Chung; Park,Hyung Soo; Lim,Young Chul; Lee,Sang-Hoon. |
| In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium; Forage crop; Italian ryegrass; Transgenic plants. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008 |
| |
|
|
| Khan,Mohammad Sayyar; Ahmad,Dawood; Khan,Muhammad Adil. |
| Global agriculture in the context of growing and expanding populations is under huge pressure to provide increased food, feed, and fiber. The recent phenomenon of climate change has further added fuel to the fire. It has been practically established now that the global temperature has been on the increase with associated fluctuations in annual rainfall regimes, and the resultant drought and flood events and increasing soil and water salinization. These challenges would be met with the introduction and utilization of new technologies coupled with conventional approaches. In recent years, transgenic technology has been proved very effective in terms of production of improved varieties of crop plants, resistant to biotic stresses. The abiotic stresses such as... |
| Tipo: Journal article |
Palavras-chave: Osmoprotectants; Salt stress; Tolerance; Transgenic plants. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400001 |
| |
|
| |
|
|
| Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa. |
| There is evidence that overexpression of transgenes codifying antifungal proteins may confer protection to pathogen attack, and that this protection is increased due to the synergic effect of the expression of two or more genes. On the other hand it is well known that sunflower is a recalcitrant specie, highly difficult to be genetically transformed. In this context, the final aim of this project was to obtain sunflower plants expressing at least two antifungal genes, avoiding sequential transformation. The antifungal genes used encode for two enzymes that degrade the fungal wall (glucanase and chitinase), an osmotin and a ribosome inhibitor protein. Two types of transformation vectors were used: a more traditional system with a double cassette and a novel... |
| Tipo: Journal article |
Palavras-chave: Antifungal genes; Double expression cassettes; Polyprotein cassettes; Sunflower; Transgenic plants. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500008 |
| |
|
|
| Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena. |
| Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
| |
|
|
| Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.. |
| Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant development; Functional study; Transgenic plants; Mutants; Urease. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209 |
| |
|
| |
|
| |
|
|
| CARNEIRO, G. E. de S.; PIPOLO, A. E.; MELO, C. L. P. de; LIMA, D. de; FOLONI, J. S. S.; MIRANDA, L. C.; PETEK, M. R.; BORGES, R. de S.; GOMIDE, F. B.; DALBOSCO, M.; DENGLER, R. U.. |
| Estabilidade: a BRS 388RR é uma soja precoce com bom desempenho em diferentes épocas de semeadura e ambientes de produção. Apresenta tipo de crescimento indeterminado, arquitetura moderna e alta produtividade com estabilidade. Grupo de maturidade relativa. Regiões edafoclimáticas de adaptação. Produtividade. Sanidade. |
| Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) |
Palavras-chave: Soja; Variedade; Planta transgênica; Soybeans; Cultivars; Transgenic plants. |
| Ano: 2014 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1003665 |
| |
|
| |
|
| |
|
|
| MELO, C. L. P. de; PIPOLO, A. E.; MOREIRA, J. U. V.; LIMA, D. de; FOLONI, J. S. S.; MIRANDA, L. C.; PETEK, M. R.; BORGES, R. de S.; GOMIDE, F. B.; DALBOSCO, M.; DENGLER, R. U.. |
| BRS 413RR: soja precoce com estabilidade e sanidade. Grupo de maturidade relativa; Regiões edafoclimáticas de adaptação; ciclo, acamamento e densidade de plantas; características; Época de semeadura; Reação a doenças. |
| Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) |
Palavras-chave: Soja; Planta transgênica; Variedade; Soybeans; Transgenic plants; Cultivars. |
| Ano: 2016 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1052529 |
| |
|
| |
|
| |
|
| |
|
|
| CALVO, E. S.. |
| Biotecnologia e o melhoramento genético de plantas. Mas o que é engenharia genética? Como assim? Como é o DNA? Mas como desenvolver novas variedade de plantas via engenharia genética? Como é feita a transformação? Transformação via agrobactéria e regeneração de plantas por organogênese. Como é feita regeneração de plantas num tubo de ensaio? Transformação com o revólver de genes. De que outra maneira a biotecnologia pode auliar no desenvolvimento de novas variedades? Regeneração de plantas de soja via embriogênese somática. Mas como os genes são identificados? O que é um organismo mutante? Como criar um mutante? Existem riscos associados com o uso de plantas transgênicas? |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Soja; Planta transgênica; Biotecnologia; Soybeans; Transgenic plants. |
| Ano: 1998 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/460384 |
| |
| Registros recuperados: 39 | |
|
|
|
