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| Registros recuperados: 16 | |
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| Oliva,Maria Luiza V.; Sampaio,Misako U.. |
| Obtained from leguminous seeds, various plant proteins inhibit animal proteinases, including human, and can be considered for the development of compounds with biological activity. Inhibitors from the Bowman-Birk and plant Kunitz-type family have been characterized by proteinase specificity, primary structure and reactive site. Our group mostly studies the genus Bauhinia, mainly the species bauhinioides, rufa, ungulata and variegata. In some species, more than one inhibitor was characterized, exhibiting different properties. Although proteins from this group share high structural similarity, they present differences in proteinase inhibition, explored in studies using diverse biological models. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bowman-Birk; Chymotrypsin; Kunitz inhibitors; Plasma kallikrein; Primary structure; Trypsin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300023 |
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| SIMÃO,ANDERSON A.; MARQUES,TAMARA R.; MARCUSSI,SILVANA; CORRÊA,ANGELITA D.. |
| ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicinal plants; Obesity; Α-amylase; Α-glycosidase; Lipase; Trypsin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502155 |
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| Pereira,M.T.; Silva-Alves,J.M.; Martins-José,A.; Lopes,J.C.D.; Santoro,M.M.. |
| Serine-proteases are involved in vital processes in virtually all species. They are important targets for researchers studying the relationships between protein structure and activity, for the rational design of new pharmaceuticals. Trypsin was used as a model to assess a possible differential contribution of hydration water to the binding of two synthetic inhibitors. Thermodynamic parameters for the association of bovine ß-trypsin (homogeneous material, observed 23,294.4 ± 0.2 Da, theoretical 23,292.5 Da) with the inhibitors benzamidine and berenil at pH 8.0, 25ºC and with 25 mM CaCl2, were determined using isothermal titration calorimetry and the osmotic stress method. The association constant for berenil was about 12 times higher compared to the one for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Benzamidine; Berenil; Calorimetry; Protein modeling; Trypsin; Osmotic stress. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100005 |
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| Boechat,L.H.B.; Zollner,R.L.. |
| Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autoantibodies; Autoimmunity; Thyroglobulin; Trypsin; Thyroiditis; Proteases. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400012 |
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| Salbego,Joseânia; Pretto,Alexandra; Silva,Vera Maria Machado da; Loro,Vania Lucia; Lazzari,Rafael; Gioda,Carolina Rosa; Baldisserotto,Bernardo. |
| The effects of glyphosate, a nonselective herbicide (1.0 or 5.0mg L-1) on digestive enzymes activity (stomach and intestine) were evaluated in juveniles of piava (Leporinus obtusidens) after 90 days of exposure. The activity of acid protease, trypsin, chymotrypsin and amylase increased with the increase of glyphosate concentration. These results indicate that glyphosate affects digestive enzyme activities in this species, and may be an indicator of poor nutrient availability when fish survive in herbicide-contaminated water. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Herbicide; Acid protease; Amylase; Trypsin. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901603 |
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| Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia. |
| ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406 |
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| Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
| Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
| Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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| Bergeron, Jean-pierre. |
| The study examines the spatial variations over the French continental shelf of the southern Bay of Biscay (North-East Atlantic) in the nutritional condition of anchovy (Engraulis encrasicolus L.) larvae which was estimated through their DNA/C index. The same cruise yielded quantitative estimates of two metabolic descriptors of the functioning of mesozooplanktonic communities, the enzymes pyruvate kinase (PK) and trypsin, for the first time evaluated simultaneously. The aim was to appraise quality and quantity of matter assimilated by the mesozooplankton and to specify whether the main components of prey ingested by copepods are principally carbohydrates, through PK activity, or protein, under the action of the digestive endopeptidase trypsin. The larval... |
| Tipo: Text |
Palavras-chave: Anchovy larvae; Bay of Biscay; DNA/C index; Mesozooplankton; Pyruvate kinase; Trypsin. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6473.pdf |
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| Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R. |
| Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA... |
| Tipo: Text |
Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf |
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| Guyonnet, Vincent; Johnson, Joyce K.; Long, Peter L.. |
| The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS) added with 0.5% chicken bile extract in a 5% C02 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella (Expt.1). No excystation was observed under these conditions. Sporocysts were also incubated under the same conditions without pretreatment in C02. Excystation was observed for sporocysts incubated with either trypsin, chymotrypsin or pancreatic elastase, the best percentage of excystation being... |
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Palavras-chave: Chymotrypsin; Eimeria tenella; Elastase; Excystation; Trypsin. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/155 |
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| Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo. |
| ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322 |
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| Registros recuperados: 16 | |
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