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| Registros recuperados: 37 | |
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| Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男. |
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Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314 |
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| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南. |
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Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311 |
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| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
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Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
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| Sivakumar, T; Khukhuu, A; Igarashi, I; Xuan, X; Guo, G; Huang, X; Yokoyama, N; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| A molecular epidemiological survey on Theileria orientalis was conducted in a cattle population of Fujian province in China. The screening polymerase chain reaction assay showed that 23 (45%) of 51 blood samples were positive for T. orientalis. DNA sequencing of the major piroplasm surface antigen-encoding gene indicated the presence of 3 different genotypes in the study area(types 1，2，and 5) and identified T. orientalis types 1 and 2 as the major genotypes in the sample population. Because type 2 (Ikeda type in Japan) has been recognized as a relatively virulent genotype of T. orientalis，control and preventive measures to minimize the incidence of infectious diseases among cattle bred in China are necessary. |
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Palavras-chave: Theileria orientalis; Cattle; China; Epidemiology; Genotype. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3098 |
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| Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南. |
| The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection. |
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Palavras-chave: Babesia gibsoni; Diagnosis; ELISA. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925 |
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| YU, Longzheng; TERKAWI, Mohmad Alaa; CRUZ-FLORES, Mary Jane; G. CLAVERIA, Florencia; ABOGE, Gabriel Oluga; YAMAGISHI, Junya; GOO, Youn-Kyoung; CAO, Shinuo; MASATANI, Tatsunori; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| A total of 250 blood samples were collected from clinically healthy cattle in five provinces of Philippines. DNA was extracted from the samples and analyzed by nested PCR assays for an epidemiological survey of Babesia bovis and Babesia bigemina infections. Out of the 250 samples, 27 (10.8%) and 16 (6.4%) were positive for B. bovis infection and B. bigemina infection, respectively. Mixed infections were detected in a total of 4 samples (1.6%). Our data provide baseline information regarding the epidemiology of B. bovis and B. bigemina infections in cattle in Philippines, which can be utilized in developing proper strategies for disease control and management. |
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Palavras-chave: Babesia bigemina; Babesia bovis; NPCR; Philippines. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3919 |
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| Chahan, Bayin; Jian, Zijian; Xuan, Xuenan; Sato, Yukita; Kabeya, Hidenori; Tuchiya, Kotaro; Itamoto, Kazuhito; Okuda, Masaru; Mikami, Takeshi; Maruyama, Soichi; Inokuma, Hisashi; 玄, 学南; 猪熊, 壽. |
| Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. |
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Palavras-chave: Anaplasma; Ehrlichia domestic animals seroepidemiology Xinjiang Uygur Autonomous Region China. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/742 |
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| YU, Longzheng; ZHANG, Shoufa; LIANG, Wanfeng; JIN, Chunmei; JIA, Lijun; LUO, Yuzi; LI, Yan; CAO, Shinuo; YAMAGISHI, Junya; NISHIKAWA, Yoshifumi; KAWANO, Suguru; FUJISAKI, Kozo; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China. |
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Palavras-chave: Major piroplasm surface protein; PCR; Theileria. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3586 |
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| 久保田, 直樹; 坂田, 義美; 宮崎, 直美; 板本, 和仁; 坂内, 天; 西川, 義文; 玄, 学南; 猪熊, 壽; NISHIKAWA, Yoshifumi; XUAN, Xuenan; INOKUMA, Hisashi. |
| 組換抗原を用いた種特異的ELISAにより,犬のNeospora caninum感染について全国的血清疫学調査を実施した.1,206頭中全国30県から126頭(10.4%)が陽性を示した.陽性率は,雄よりも雌で有意に高く,また他品種と比較して有意に高い陽性率を示す品種がみられた.子宮蓄膿症および糖尿病の病歴を有する犬の陽性率は,他の疾病を有する犬または疾病のない犬の陽性率に比べて高値であった. A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other... |
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Palavras-chave: Canine; Nbospora caninum; Species-specific ELISA. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4003 |
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| Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男. |
| In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that... |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631 |
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| 玄, 学南; XUAN, XUENAN. |
| 本研究では、プロテオミックス法によるネオスポラ原虫の全虫体タン パク質の網羅的解析を行った。MALDI-TOF-MS 解析により約600 種類の可視化されたタンパク質 の中から約60 種類のタンパク質の同定に成功した。そのうちの一部は原虫の宿主細胞への吸 着・侵入ならびにエネルギー代謝に関わるタンパク質であることが示唆された。これらの成果 は、ネオスポラ原虫の新規ワクチン・治療薬開発に繋がるものとして期待される。 The aims of this study were to widen our view on the global expression of protein profile of Neospora caninum parasite and to discover vaccine and drug target molecules for control of neosporosis. Approximately 600 protein spots were detected on 2-DE, and 60 proteins were identified by MALDI-TOF-MS analysis. Some of the identified proteins were predicted to be involved in the invasion of the parasite into the host cells and in parasite metabolism. Therefore, some of these proteins could be potential vaccine candidates and drug targets. |
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Palavras-chave: ネオスポラ; トキソプラズマ; プロテオミックス; ワクチン; 治療薬; MALDI-TOF-MS; 抗原; 酵素. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2759 |
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| Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%).... |
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Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142 |
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| MASATANI, Tatsunori; ASADA, Masahito; ICHIKAWA-SEKI, Madoka; USUI, Miho; TERKAWI, Mohamad A.; HAYASHI, Kei; KAWAZU, Shin-ichiro; XUAN, Xuenan; 河津, 信一郎; 玄, 学南. |
| Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni. |
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Palavras-chave: Antioxidant activity; Babesia gibsoni; Canine; Peroxiredoxin. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3925 |
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| Terkawi, M. A.; Aboge, G.; Jia, H.; Goo, Y-K.; Ooka, H.; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawazu, Shin-ichiro; Fujisaki, K.; Xuan, Xuenan; 山岸, 潤也; 西川, 義文; 河津, 信一郎; 玄, 学南. |
| A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both... |
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Palavras-chave: Babesia gibsoni; Enzyme-linked immunosorbent assay; Deiagnostic performance. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2232 |
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| Registros recuperados: 37 | |
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