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| Dusi,André N.; Carvalho,César; Torres,Antônio Carlos; Ávila,Antonio Carlos de. |
| Two transgenic potato clones of cv. Achat, denominated 1P and 63P were challenged with two Potato virus Y strains (PVY O and PVY N), under greenhouse conditions, to be evaluated for resistance to these strains. Optical density values of the Elisa readings of samples from the transgenic plants were compared to readings from samples of the inoculated non-transformed plants. Clone 1P was extremely resistant to both PVY strains, reflected by not being systemically infected. Clone 63P, however, presented partial resistance to both PVY strains as local or systemic infection was delayed in some days. These results confirm the previously reported extreme resistance to PVY of clone 1P. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; GMO; Extreme resistance. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000300012 |
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| Fonseca,Leonardo N.; Inoue-Nagata,Alice K.; Nagata,Tatsuya; Singh,Rudra P.; Ávila,Antonio Carlos de. |
| O Potato virus Y (PVY) tornou-se o maior problema nas áreas de produção de batata semente do Brasil. Somente as estirpes comum (PVYº) e necrótica (PVY N) eram detectadas infectando batata no Brasil. Esta situação mudou drasticamente a partir de 1997 quando um surto epidêmico de uma variante da estirpe necrótica de PVY causando arcos e anéis necróticos na superfície do tubérculo (PVY NTN) foi observado no país. Este estudo visou avaliar e validar uma metodologia de diferenciação de estirpes que causam necrose em tubérculos, proposta por Weilguny e Singh (1998). Vinte e oito isolados de PVY originários de tubérculos infectados de batata, provenientes de quatro estados brasileiros, foram analisados em sua reação em plantas de fumo, por Elisa, utilizando... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Vírus Y da batata; Reação em cadeia da polimerase; Potyvírus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362005000400008 |
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| Inoue-Nagata,Alice Kazuko; Martin,Darren Patrick; Boiteux,Leonardo Silva; Giordano,Leonardo de Britto; Bezerra,Isabel Cristina; Ávila,Antonio Carlos de. |
| Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with Tomato mottle leaf curl virus (TMoLCV), isolates 34, PA-05, and Ta4 were 88% identical to Tomato yellow vein streak virus and isolate DF-BR3 shared 77% identity with TMoLCV. Recombination analysis indicated that isolate DF-BR3 was a chimaera, and it provided evidence that there is a complex and actively recombining population of tomato infecting begomoviruses in Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Begomovirus; Geminivirus; Virus recombination. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000800018 |
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