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Registros recuperados: 57 | |
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松山, 雄喜; 神尾, 恭平; 村上, 智亮; 下田, 崇; 古林, 与志安; 古岡, 秀文; 松本, 高太郎; 猪熊, 壽; MATSUYAMA, Yuuki; KAMIO, Kyouhei; MURAKAMI, Tomoaki; SHIMODA, Takashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUMOTO, Kohtaro; INOKUMA, Hisashi. |
5 歳6 カ月齢ホルスタイン種乳牛が,後躯麻痺による起立不能を呈した.浅頸リンパ節の腫大と直腸検査による腫瘤 の触知から牛白血病を疑ったが,血液検査とリンパ節の針吸引生検では確定診断できなかった.剖検では,60 × 40 × 20cm 大の多結節性腫瘤が背側の腹腔壁に接して存在していた.腫瘍組織は腸腰筋に浸潤し,一部は第5 および第6 腰 椎椎間孔より脊柱管内に連続的に波及し,脊髄および脊髄神経根を圧迫していた.腫瘍はCD3 陽性のT細胞性リンパ 腫で,牛白血病ウイルス抗体陰性であったため,散発型牛白血病と診断された. A Holstein cow (5 years and 3 months old) presented with hind limb paresis and was unable to stand. Bovine leukosis was suspected given the enlargement of cervical lymph nodes and existence of an abdominal mass detected by rectal palpation; however,it could not be confirmed by blood examination and fine needle aspiration of cervical lymph nodes. Pathological examination revealed a mu1tinodular mass (60 x 40 x 20 cm) on the dorsal abdomen that was invasive into the iliopsoas muscle. A portion of the... |
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Palavras-chave: 牛白血病; 脊髄浸潤; 後躯麻痺; Bovine leukosis; Spinal cord invasion; Hind limb paresis. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2809 |
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HORIUCHI, Noriyuki; KUMAGAI, Daishiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安. |
Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C>T in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to direct sequences. The homogeneous c.343C>T mutation was proved to occur in all the affected cows and not in the control cows. The present... |
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Palavras-chave: Cattle; Dilated cardiomyopathy; Nonsense mutation; OPA3 gene. |
Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4208 |
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YBAÑEZ, Adrian Patalinghug; SATO, Fumio; NAMBO, Yasuo; FUKUI, Takashi; MASUZAWA, Toshiyuki; OHASHI, Norio; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; INOKUMA, Hisashi; 松本, 高太郎; 猪熊, 壽. |
A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time. |
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Palavras-chave: Anaplasma sp.; Borrelia sp.; Rickettsia sp.; Thoroughbred horse; Tick-borne diseases. |
Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3840 |
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LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝. |
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination. |
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Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis. |
Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891 |
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TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan. |
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Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep. |
Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582 |
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齋藤, 亨; 中川, 大輔; 富田, 健介; 古林, 与志安; 松本, 高太郎; 猪熊, 壽. |
ホルスタイン雄子牛が起立不能、努力性呼吸、チアノーゼを呈した。また、心雑音も聴取されたことから先天性心疾患を疑った。心音図検査より左側第2~3肋間の心基底部を最強点とする拡張期雑音および収縮前期雑音が確認された。心エコー検査では大動脈騎乗と心室中隔欠損が認められ、また肺動脈領域には著しく拡張した流出路と弁の閉鎖不全が認められた。動脈血液ガス検査では、酸素分圧26.25mmHg、酸素飽和度は測定限界の60%未満という著しい低値が認められた。病理解剖により高位心室中隔欠損、卵円孔開存および右心室壁の高度肥厚が認められた。また、心臓からは左右の心室腔へ騎乗する拡張した大動脈だけが起始し、肺動脈は閉鎖していた。以上の所見から本症例はファロー五徴極型と診断された。 |
Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3551 |
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猪熊, 壽; 滄木, 孝弘; 松本, 高太郎; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; MATSUMOTO, Kotaro; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi. |
Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4145 |
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坂本, 礼央; 大林, 哲; 古林, 与志安; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽; SAKAMOTO, Leo; OHBAYASHI, Tetsu; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi. |
チミジンキナーゼはDNA合成に関わる酵素の一つであり,近年牛白血病の発症マーカーとして利用できることが報 告されている.今回,牛白血病ウイルス汚染農場において,本酵素の活性を測定することにより,牛白血病罹患牛の早期摘発を試みた.その結果,臨床症状は示さなかったものの,地方病性牛白血病に罹患していた10歳9カ月齢のホルスタイン種雌牛を摘発できた.牛白血病ウイルス汚染牛群における本酵素活性の測定が,地方病性牛白血病罹患牛の早期摘発に臨床上有用であることが本研究により示唆された. Thymidine kinase is an enzyme related to DNA synthesis. Recently,serum thymidine kinase (STK) activity has been reported as a useful marker of bovine leukosis. In an effort to achieve earlier detection of enzootic bovine leukosis (EBL),we measured STK activity in cows at a farm tainted by bovine leukemia virus (BLV). We found that a Holstein cow aged 10 years and 9 months without any clinical symptoms showed higher STK activity,and the cow was diagnosed as having EBL. Evaluation of STK activity in a herd contaminated... |
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Palavras-chave: 地方病性牛白血病; 早期摘発; 血清チミジンキナーゼ活性; Early case detection; Enzootic bovine leukosis; Serum thymidine kinase activity. |
Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2942 |
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CHIBA, Shiori; FUNATO, Shingo; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安. |
Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of... |
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Palavras-chave: Axonal degeneration; Gliosis; Japanese black cattle; Optic pathway degeneration. |
Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4123 |
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YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The... |
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Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL. |
Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839 |
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Sashika, Mariko; Abe, Go; Matsumoto, Kotaro; Inokuma, Hisashi; 松本, 高太郎; 猪熊, 壽. |
Rickettsial infection in feral raccoons (Procyon lotor) in Hokkaido, Japan was analyzed by molecular methods. Genus-specific nested polymerase chain reaction (PCR) analysis based on the Rickettsia citrate synthase (gltA) gene showed that 13 of 699 raccoons (1.9%) examined were positive for Rickettsia. Twelve of the 13 partial gltA sequence amplicons were successfully analyzed. The nucleotide sequence of one amplicon was identical to both Rickettsia heilongjiangensis and R. japonica, one was identical to R. felis, and the rest to R. helvetica. This is the first report on the detection of rickettsial agents in peripheral blood of raccoons. |
Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3087 |
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高垣, 勝仁; 池川, 晃世; 二宮, 理沙; 堀内, 雅之; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; HORIUCHI,Noriyuki; MATSUMOTO,Kotaro; KOBAYASHI,Yoshiyasu; INOKUMA,Yasushi. |
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Palavras-chave: 心室中隔欠損; 多発性疣贅性心内膜炎; 心膜炎; ホルスタイン種. |
Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4144 |
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岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka. |
牛臨床におけるComputed Radiography (CR) の有用性を確立するため,管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性,鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが,粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく, IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results,it was concluded that accidental variations in the tube-cassette... |
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Palavras-chave: 牛; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator. |
Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939 |
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竹内, 俊彦; 吉本, 薫; 駒形, 真; 福中, 守人; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; TAKEUCHI, Toshihiko; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; FUKUNAKA, Morito; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi. |
5歳5カ月齢のホルスタイン種雌牛が,発熱,食欲不振及び胎盤停滞を呈した.悪露排出及び子宮の腫脹は治療で改善がみられなかった.体表リンパ節の腫大はみられなかったが,直腸検査で子宮の硬結を認めたため,牛白血病を疑った.病理解剖では腹腔内リンパ節の腫大,胃漿膜面の腫瘤形成及び子宮壁の肥厚が認められた.また,組織所見ではこれら組織へのリンパ球様腫瘍細胞の浸潤が認められた.腫瘍細胞はB細胞マーカー陽性であった.牛白血病ウイルス(BLV)抗体は陰性であったが,末梢血のBLVプロウイルス検査では陽性が確認され,本症例におけるBLVの関与が示唆された. A 5-year-5-month old Holstein cow was presented with fever, anorexia, and retained placenta. Postnatal vaginal discharge and enlargement of the uterus did not improve after treatment. Although the superficial lymph nodes were not enlarged, enzootic bovine leukosis was suspected because of the enlarged and indurated uterus. An autopsy revealed enlargement of most abdominal lymph nodes, tumor mass formation on the omasum and abomasum, and thickening of the uterine wall. Histologically, there... |
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Palavras-chave: 牛白血病ウイルス; 慢性子宮内膜炎; 地方病性牛白血病; Bovine leukosis virus; Chronic endometritis; Enzootic bovine leukosis. |
Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3555 |
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Registros recuperados: 57 | |
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