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| Pereira, P. M. C.; Lima, J. Y. O.; Barros, R. F.; Ferreira, J. S.; Ikeda, M. A. K.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Asano, K. M.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| Bats found in Brazil are classified in 9 families, 64 genus and 168 species. They are an important rabies reservoirs and all species can be infected or be the transmitter of rabies virus, independently of their specie classification or feeding habits. This work aimed a survey of bats species originated from counties of Sao Paulo state, which were sent to the diagnostic laboratory of the Pasteur Institute – Sao Paulo. Between August 2009 to October 2012; 9.109 bats specimens were received and submitted to recommended techniques for rabies diagnosis by World Health Organization. From these specimens, 8.421 (92,45%) were classified in family, genus and specie, by morphological and morphometric parameters using bats identification keys. Furthermore, they also... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17386 |
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| Ikeda, M. A. K.; Barros, R. F.; Lima, J. Y. O.; Ferreira, J. S.; Pereira, P. M. C.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| The bats are a major reservoir of rabies virus and have a relevant importance in the disease transmission. The aim of this study was to evaluate the bat population submitted for rabies diagnosis. Data were analyzed from 18.805 bats received by the diagnostic section of the Pasteur Institute of Sao Paulo, originated from several counties of the state of Sao Paulo over the period from 2007 to 2012. These specimens were morphologically classified according to their family, at the time of execution of the rabies diagnosis technique. The central nervous system from these animals was submitted to direct immunofluorescence test and, when viable, to viral isolation. From the total of bats received, 76.92% bats belonged to the Molossidae family, 12.14% to the... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17383 |
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| Achkar, S. M.; Fernandes, E. R.; Guedes, F.; Mori, E.; Del Fava, C.; Duarte, M. I.. |
| A raiva é uma meningoencefalomielite que acomete diferentes espécies. Estudos da literatura mencionam que possa haver uma dissociação entre a presença de infecção viral e inflamação. Objetivo: caracterizar as alterações histopatológicas no sistema nervoso central (SNC) de equinos e bovinos infectados pela raiva e comparar a presença de corpúsculos de Negri pela coloração de hematoxilina‐eosina (HE) e por técnica de imunohistoquímica, correlacionando a presença do antígeno viral e a inflamação. Métodos: 05 amostras de SNC de bovinos e 05 de equinos foram analisadas pela coloração de HE. O antígeno da raiva foi pesquisado pela técnica de imunohistoquímica (IHQ), utilizando‐se anticorpo policlonal antivírus da raiva e método Estreptavidina‐biotina peroxidase.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17384 |
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| Lima, J. Y. O.; Scheffer, K. C.; Achkar, S. M.; Kotait, I.; Carrieri, M. L.. |
| The diversity of bat species in Brazil is great, and there are 172 species distributed among nine families. The Phyllostomidae family is the most numerous, followed by Vespertilionidae and Molossidae. According to feeding habits, the majority of bats are insectivorous, followed by frugivorous, nectarivorous, carnivorous and hematophagous. As these animals are considered reservoirs of rabies virus it is essential to correctly identify the species and knowledge of the c rmv s p . g o v . b r mv&z 79 biology, behavior and adaptation of bats in urban environments. The objective of this study was to identify the several specimens of bats received for rabies diagnosis at the Pasteur Institute, meet the positive index of them and assess their distribution on... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3079 |
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| Carrieri, M. L.; Achkar, S. M.; Castilho, J. G.; Oliveira, R. N.; Carnieli Jr, P.; Macedo, C. I.; Omoto, T. M.; Takaoka, N. Y.; Kotait, I.. |
| The São Paulo State Rabies Control Program, coordinated by the Pasteur Institute of São Paulo, has been involved in endeavors to improve the epidemiological surveillance of rabies in dogs, cats and bats, particularly in urban areas. Since 1998, when the last case of canine rabies due to antigenic variant 2 occurred in the state, the Institute has received 66,352 canine central nervous system samples, 17,796 samples from cats and 38,133 from bat specimens. Using conventional techniques for rabies diagnosis, 11 dogs, 8 cats and 554 bats were found to be positive for this disease. When molecular techniques, such as antigenic typing and genetic sequencing, were used, antigenic variant 3, which is associated with Desmodus rotundus, was detected in 11 dogs and 7... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2566 |
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| Lima, J. Y. O.; Achkar, S. M.; Scheffer, K. C.; Castilho, J. G.; Rodrigues, A. C.; Kotait, I.; Carrieri, M. L.. |
| Bats are considered important reservoirs of rabies virus, which is paramount in the study of the pathogenesis of this zoonosis disease, through the use of sensitive systems. The presence of viral antigen in these species shows that non-neuronal viral spread is efficient in different organs that participate effectively in the elimination of rabies virus, such as, salivary glands and bladder. This study aimed to investigate the presence of rabies virus in samples of bats submitted for laboratory diagnosis, as well as, to study the pathogenesis of the disease through the use of laboratory animals. 3,930 routine diagnostic specimens of bats were processed during the period between January 2011 and May 2012 by direct immunofluorescence (DIF) and viral isolation... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3078 |
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| Jarcovis, R. L. M.; Gamon, T. H. M.; Nogi, K. I.; Fahl, W. O.; Asano, K. M.; Achkar, S. M.; Maiorka, P. C.; Mori, C. M. C.; Mori, E.. |
| Introdução e Objetivos: a prova biológica (PB) de inoculação intracerebral (IC) em camundongos com o vírus da raiva (RABV) apresenta sensibilidade diagnóstica superior ao teste de imunofluorescência direta (IFD). No entanto, o tempo necessário para o diagnóstico pela PB é maior que o consumido pela IFD, pois é dependente do período de incubação (PI) viral após inoculação IC. A determinação da duração desses períodos de observação (PO) em camundongos após inoculação IC com RABV foi estabelecida por observações empíricas. Com o intuito de estabelecer a duração do PO dos camundongos da linhagem Swiss inoculados IC com amostras clínicas (SNC, saliva e líquor) positivas para raiva, provenientes de diferentes espécies animais e humanos, oriundos do setor de... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25665 |
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