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Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar. |
Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007 |
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