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In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil BJM
Aguiar,Daniel M.; Hagiwara,Mitika K.; Labruna,Marcelo B..
An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
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Anti-Leptospira spp and anti-Brucella spp antibodies in humans from rural area of Monte Negro municipality, state of Rondônia, Brazilian Western Amazon BJM
Aguiar,Daniel M.; Cavalcante,Guacyara T.; Camargo,Luis M. A.; Labruna,Marcelo B.; Vasconcellos,Silvio A.; Souza,Gisele O.; Gennari,Solange M..
Sera from 276 humans living in 71 farms located in Monte Negro Municipality, RO., Western Amazon, Brazil were examined for anti-Leptospira spp antibodies by Microscopic Agglutination Test and for anti-Brucella spp antibodies by Tube Agglutination Test. Leptospira spp antibodies were detected in 28 (10.2%) of them with at least one positive case in 23 farms (32.4%). The most frequent leptospira serovars were Patoc (46.7%), Autumnalis (10.0%) and Shermani (10.0%). The proportion of positive males (14.5%) were higher than females (5.0%; P <0.05) and the contact with river water presented association with Leptospira spp infection (OR: 27; P=0.01). A total of 04 (1.4%) humans reacted against Brucella antigens; three farms (4.2%) presented at least one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human; Leptospirosis; Brucellosis; Amazon; Brazil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100019
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