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Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar BABT
Montañez Sáenz,Julio; Téllez,Alejandro; de la Garza,Heliodoro; de la Luz Reyes,María; Contreras-Esquivel,Juan Carlos; Aguilar,Cristóbal Noé.
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extraction; Purification; Properties; Pectinesterase; Potato.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007
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Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods BJM
Paz,Jorge Enrique Wong; Contreras,Carolina Rubio; Munguía,Abigail Reyes; Aguilar,Cristóbal Noé; Inungaray,María Luisa Carrillo.
Abstract Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S. paratyphi, and by the Minimum Bactericidal Concentration, the percentage of activity and the Index of Bacterial Susceptibility of each extract. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial activity; Hamelia patens; Polyphenols; HPLC-MS.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300656
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