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| Massa, Sonia I.; Pearson, Gareth A.; Aires, Tania; Kube, Michael; Olsen, Jeanine L.; Reinhardt, Richard; Serrao, Ester A.; Arnaud Haond, Sophie. |
| Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed... |
| Tipo: Text |
Palavras-chave: EST library; High temperature stress; Abiotic stress response; Climate change. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00044/15559/13326.pdf |
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| Aires, Tania; Serrao, Ester A.; Kendrick, Gary; Duarte, Carlos M.; Arnaud-haond, Sophie. |
| Biological invasions rank amongst the most deleterious components of global change inducing alterations from genes to ecosystems. The genetic characteristics of introduced pools of individuals greatly influence the capacity of introduced species to establish and expand. The recently demonstrated heritability of microbial communities associated to individual genotypes of primary producers makes them a potentially essential element of the evolution and adaptability of their hosts. Here, we characterized the bacterial communities associated to native and non-native populations of the marine green macroalga Caulerpa racemosa through pyrosequencing, and explored their potential role on the strikingly invasive trajectory of their host in the Mediterranean. The... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26791/24886.pdf |
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| Aires, Tania; Marba, Nuria; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie. |
| Molecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photosynthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green... |
| Tipo: Text |
Palavras-chave: 16S; Chloroplasts; Elimination; Green algae; Metagenomics. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00076/18739/16464.pdf |
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| Aires, Tania; Moalic, Yann; Serrao, Ester; Arnaud-haond, Sophie. |
| The siphonous algae of the Caulerpa genus harbor internal microbial communities hypothesized to play important roles in development, defense and metabolic activities of the host. Here, we characterize the endophytic bacterial community of four Caulerpa taxa in the Mediterranean Sea, through 16S rRNA amplicon sequencing. Results reveal a striking alpha diversity of the bacterial communities, similar to levels found in sponges and coral holobionts. These comprise (1) a very small core community shared across all hosts (< 1% of the total community), (2) a variable portion (ca. 25%) shared by some Caulerpa taxa but not by all, which might represent environmentally acquired bacteria and (3) a large (> 70%) species-specific fraction of the community,... |
| Tipo: Text |
Palavras-chave: Endophytic bacteria; Bacterial community; Cooccurrence network; Modularity; Coevolution. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00277/38800/37699.pdf |
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| Aires, Tania; Marba, Nuria; Cunha, Regina L.; Kendrick, Gary A.; Walker, Diana I.; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie. |
| Seagrasses are the structural species of one of the most important coastal ecosystems worldwide and support high levels of biodiversity and biomass production. Posidonia is one of the most ancient seagrass genera and displays a contrasting disjunct biogeographic pattern. It contains one single species in the Northern Hemisphere, P. oceanica, which is endemic to the Mediterranean Sea, and has up to 8 recognized taxa in the Southern Hemisphere, which in Australia are divided into 2 complexes, P. ostenfeldii and P. australis. A phylogeny based on a nuclear marker (rRNA-ITS) revealed an ancient split between the northern (i.e. Mediterranean) and southern (i.e. Australian) taxa, followed by a separation of the 2 recognized Australian complexes. However, the... |
| Tipo: Text |
Palavras-chave: Posidonia; Phylogeny; Low evolutionary rates; Ancient diversification. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00027/13798/10937.pdf |
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