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| Registros recuperados: 35 | |
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| Cheray, Mathilde; Etcheverry, Amandine; Jacques, Camille; Pacaud, Romain; Bougras-cartron, Gwenola; Aubry, Marc; Denoual, Florent; Peterlongo, Pierre; Nadaradjane, Arulraj; Briand, Josephine; Akcha, Farida; Heymann, Dominique; Vallette, François M.; Mosser, Jean; Ory, Benjamin; Cartron, Pierre-françois. |
| Background Literature reports that mature microRNA (miRNA) can be methylated at adenosine, guanosine and cytosine. However, the molecular mechanisms involved in cytosine methylation of miRNAs have not yet been fully elucidated. Here we investigated the biological role and underlying mechanism of cytosine methylation in miRNAs in glioblastoma multiforme (GBM). Methods RNA immunoprecipitation with the anti-5methylcytosine (5mC) antibody followed by Array, ELISA, dot blot, incorporation of a radio-labelled methyl group in miRNA, and miRNA bisulfite sequencing were perfomred to detect the cytosine methylation in mature miRNA. Cross-Linking immunoprecipiation qPCR, transfection with methylation/unmethylated mimic miRNA, luciferase promoter reporter plasmid,... |
| Tipo: Text |
Palavras-chave: MiRNA; Cytosine-methylation; Epigenetics; DNMT3A; AGO4; Glioblastoma; Epitranscriptomics. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00610/72196/70980.pdf |
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| Bean, Tp; Akcha, Farida. |
| Single cell gel electrophoresis (SCGE or comet) assay allows quantification of DNA damage in individual cells and is an ideal tool for use within biological monitoring programmes. Comet assay can be used on a range of cell types including somatic, reproductive (gametes) or circulatory cells in many different species including both marine bivalves and flatfish. The assay can be employed with simple equipment available in most laboratories, is sensitive to environmentally relevant levels of DNA damage (Frenzilli and Lyons, 2009), accurately demonstrates a linear dose response to exposure (Collins et al., 1996), and can be adapted for use on most nucleated cell types. This document concentrates on the simplest and most repeatable method of comet assay in... |
| Tipo: Text |
Palavras-chave: Comet assay; Single cell gel electrophoresis; DNA damage; Genotoxicity; Biological effects; Bivalves; Flatfish; Integrated monitoring. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00587/69902/67807.pdf |
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| Revel, Messika; Lagarde, Fabienne; Perrein-ettajani, Hanane; Bruneau, Mélanie; Akcha, Farida; Sussarellu, Rossana; Rouxel, Julien; Costil, Katherine; Decottignies, Priscilla; Cognie, Bruno; Châtel, Amélie; Mouneyrac, Catherine. |
| The impact of a microplastic (MP) mixture composed of polyethylene (PE) and polypropylene (PP) plastic particles, prepared from commercially available products, was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevant concentrations: 0.008 μg L−1 (low), 10 μg L−1 (medium), and 100 μg L−1 (high). Organisms were exposed for 10 days followed by 10 days of depuration in clean seawater under controlled laboratory conditions. The evaluation of MP effects on mussel clearance rate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNA integrity were investigated while the identification of plastic particles in mussel tissues (gills, digestive gland, and remaining tissues), and biodeposits (feces and... |
| Tipo: Text |
Palavras-chave: Microplastics; Polyethylene; Polypropylene; Mytilus; Oxidative stress; Biomarkers. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00489/60089/63427.pdf |
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| Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine. |
| Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf |
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| Buchet, Vincent; Akcha, Farida; Stachowski-haberkorn, Sabine. |
| Il s’agissait i) de mettre en place un réseau de suivi de la qualité de l’eau, ii) de déterminer la qualité de chaque élevage larvaire dans le but de mettre en évidence un lien éventuel entre la qualité physico-chimique de l’eau de mer et la production conchylicole en écloserie, iii) mettre en évidence, l'influence de l’eau utilisée pour l’élevage, à court-terme, sur la « qualité » physiologique de l’algue fourrage T-iso utilisée pour nourrir les larves et à plus long-terme vérifier si ses capacités de croissance ainsi que sa «qualité» physiologique sont susceptibles d’être modifiées en fonction de la qualité de l’eau issue du polder. Les résultats obtenus ont mis en évidence la présence permanente de contaminants chimiques dans les eaux du polder. Les... |
| Tipo: Text |
Palavras-chave: Contaminants chimiques; Huîtres; Écloserie; Polder ostréicole; T iso. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00477/58885/62812.pdf |
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| Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Kholbystov, Andrei N; Readman, James W; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR).... |
| Tipo: Text |
Palavras-chave: Trojan Horse effect; B[a]P; NC60; Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60946/64340.pdf |
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| Killelea, Tom; Palud, Adeline; Akcha, Farida; Lemor, Mélanie; L'Haridon, Stephane; Godfroy, Anne; Henneke, Ghislaine. |
| 8-oxodeoxyguanosine (8-oxodG), a major oxidised base modification, has been investigated to study its impact on DNA replication in hyperthermophilic Archaea. Here we show that 8-oxodG is formed in the genome of growing cells, with elevated levels following exposure to oxidative stress. Functional characterisation of cell-free extracts and the DNA polymerisation enzymes, PolB, PolD, and the p41/p46 complex, alone or in the presence of accessory factors (PCNA and RPA) indicates that translesion synthesis occurs under replicative conditions. One of the major polymerisation effects was stalling, but each of the individual proteins could insert and extend past 8-oxodG with differing efficiencies. The introduction of RPA and PCNA influenced PolB and PolD in... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00502/61372/64989.pdf |
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| Mauffret, Aourell; Wessel, Nathalie; Akcha, Farida; Munschy, Catherine; Chouvelon, Tiphaine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Aminot, Yann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Menard, Dominique; Burgeot, Thierry. |
| Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Des soles ont été prélevées en août et septembre 2017 et des moules en février 2018, dans le cadre du suivi ROCCH MV. Les biomarqueurs suivis chez ces organismes couvrent plusieurs mécanismes d’actions toxiques (reprotoxicité, génotoxicité, neurotoxicité) et incluent des mesures de la santé générale de l'organisme. Les contaminants suivis sont des éléments traces métalliques et des contaminants organiques... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00620/73215/72410.pdf |
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| Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry. |
| Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation... |
| Tipo: Text |
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf |
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| Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah. |
| Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg... |
| Tipo: Text |
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf |
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| Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume. |
| Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval... |
| Tipo: Text |
Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf |
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| Wessel, Nathalie; Santos, Raphael; Menard, Dominique; Le Menach, K.; Buchet, Vincent; Le Bayon, Nicolas; Loizeau, Veronique; Burgeot, Thierry; Budzinski, H.; Akcha, Farida. |
| Polycylic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environment Their toxicity is mainly linked to the ability of marine species to biotransform them into reactive metabolites PAHs are thus often detected at trace levels in animal tissues For biomonitoring purposes this findings have two main consequences (i) the determination of the PAH tissue concentration is not suitable for the evaluation of individual exposure to PAHs (ii) It can explain sometimes the lack of correlations obtained with relevant markers of toxicity such as genotoxicity biomarkers The aim of the present study was to better investigate the link between PAH exposure and genotoxicity in marine flatfish During a laboratory experiment juvenile soles were exposed... |
| Tipo: Text |
Palavras-chave: Ecotoxicology; Sole; PAH; Metabolites; EROD; Comet assay. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12970/10834.pdf |
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| Barranger, Audrey; Benabdelmouna, Abdellah; Degremont, Lionel; Burgeot, Thierry; Akcha, Farida. |
| Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in... |
| Tipo: Text |
Palavras-chave: Aneuploidy; FISH; Diuron; Crassostrea gigas. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00238/34911/33216.pdf |
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| Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida. |
| In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00250/36157/34712.pdf |
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| Perrichon, Prescilla; Akcha, Farida; Le Menach, Karyn; Goubeau, Manon; Budzinski, Helene; Cousin, Xavier; Bustamante, Paco. |
| In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3 ×, 1 × and 3 ×; 1 × represents an environmental concentration measured in French estuary).... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbons; Parental transmission; Fish early-life stages; PhotoMotor Response; Cardiac performance; PAH metabolites; Genotoxicity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00259/37046/37174.pdf |
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| Mauffret, Aourell; Wessel, Nathalie; Roubeix, Vincent; Akcha, Farida; Chouvelon, Tiphaine; Aminot, Yann; Munschy, Catherine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Mahe, Kelig; Godfrin, Yoann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Bruzac, Sandrine; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Burgeot, Thierry. |
| Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Le présent rapport concerne le volet SELIseine. Des poissons plats (194 soles, flets et limandes) ont été prélevés en septembre 2018 sur le N/O Antéa, et des moules ont été prélevées en février 2019 sur 6 stations dans le cadre du suivi ROCCH MV suivant le protocole de la convention OSPAR (2012). Les biomarqueurs suivis chez ces organismes couvrent plusieurs types d’effets toxiques (reprotoxicité, génotoxicité,... |
| Tipo: Text |
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| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00688/80024/83012.pdf |
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| Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier. |
| Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf |
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| Revel, Messika; Châtel, Amélie; Perrein-ettajani, Hanane; Bruneau, Mélanie; Akcha, Farida; Sussarellu, Rossana; Rouxel, Julien; Costil, Katherine; Decottignies, Priscilla; Cognie, Bruno; Lagarde, Fabienne; Mouneyrac, Catherine. |
| The aim of the present study was to evaluate the presence and potential toxic effects of plastic fragments (<400 μm) of polyethylene and polypropylene on the Pacific oyster Crassostrea gigas. Oysters were exposed to environmentally relevant concentrations (0, 0.008, 10, 100 μg of particles/L) during 10 days, followed by a depuration period of 10 days in clean seawater. Effects of microplastics were evaluated on the clearance rate of organisms, tissue alteration, antioxidant defense, immune alteration and DNA damage. Detection and quantification of microplastics in oyster's tissues (digestive gland, gills and other tissues) and biodeposits using infrared microscopy were also conducted. Microplastics were detected in oyster's biodeposits following... |
| Tipo: Text |
Palavras-chave: Microplastics; Crassostrea gigas; Biodeposits; Biomarkers; Bivalves. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00588/70055/68018.pdf |
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| Registros recuperados: 35 | |
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