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Variación de fenotipos de crecimiento y de virulencia en cepas mutantes de Beauveria bassiana (Bals.) Vuill. resistentes a 2-desoxi-D-glucosa Agrociencia
Montesinos-Matías,Roberto; Viniegra-González,Gustavo; Alatorre-Rosas,Raquel; Gallardo-Escamilla,Francisco; Loera,Octavio.
En los procesos de mejoramiento de cepas de hongos entomopatógenos es necesario conocer cuáles son y cómo inciden las variables más importantes en las cepas más virulentas. En este estudio se evaluó una serie de variables de crecimiento y de virulencia de una cepa parental de Beauveria bassiana (Bals.) Vuill. (Hypocreales: Cordycipitaceae) (88) y mutantes derivadas resistentes al compuesto tóxico 2-desoxi-D-glucosa (2DG). La 2DG es un análogo tóxico de la glucosa, y se ha usado con frecuencia para aislar diversas mutantes de microorganismos desregulados en el metabolismo del carbono. De las variables evaluadas, previo al bioensayo de virulencia sobre larvas de Tenebrio molitor (Coleoptera: Tenebrionidae), el porcentaje de germinación de conidios (G) se...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Control biológico; Virulencia; Germinación; Tenebrio molitor; Análisis de Componentes Principales.
Ano: 2011 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000800006
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Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran BABT
Nuñez-Gaona,Oscar; Saucedo-Castañeda,Gerardo; Alatorre-Rosas,Raquel; Loera,Octavio.
The aim of the present work was to study the effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran (WB).The highest growth rate of B. bassiana 885.2 on WB media was obtained at a w=1.0, with no detected growth at a w < 0.97. Solid-state fermentation (SSF) using WB (66% moisture; a w=1.0) achieved a maximal yield of 1.18x10 10 conidia per gram of dry substrate (gds). This yield decreased one order of magnitude with higher moisture contents or the addition of sugarcane bagasse (SCB) as a texturiser. In SSF using WB (66% humidity),the time to obtain a yield of 1x10(10) conidia/gds, referred to as t10, could be predicted using a model considering common inoculum levels and maximal yields. For...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beauveria bassiana; Wheat bran; Water activity; Texturiser; Conidial yields.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400004
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Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae Ciencia e Investigación Agraria
Suárez-Sánchez,Juan; Alatorre-Rosas,Raquel; Poggi-Varaldo,Héctor M; Barrera-Cortés,Josefina.
The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of...
Tipo: Journal article Palavras-chave: Axenic culture; Hemoglobin; In vitro culture; Steinernema carpocapsae; Transparent medium.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011
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