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The dispersion of adhered marine bacteria by pyrophosphate and ultrasound prior to direct counting. ArchiMer
Velji, M; Albright, L.
A technique has been investigated for dispersing adhered marine bacteria from various surfaces prior to direct counting. The bacterial cells were initially preserved and strengthened by use of 3.7% formaldehyde (v/v final concentration). Seawater samples were then treated with 0.001 M of tetrasodium pyrophosphate (final concentration), a sequestering and deflocculating agent. Surface sediment and kelp disc samples were treated with 0.01 M tetrasodium pyrophosphate (final concentration) after having been suspended in 0.44 M sodium chloride solution. These samples were incubated for 15-30 min. Subsequently, the samples were treated with ultrasound (100 W) for 30 to 45 s. This technique resulted in an even dispersion of most bacterial cells in the samples....
Tipo: Text Palavras-chave: Bacteria; Microbiological analysis; Flocculation; Ultrasonics; Bacterial counters.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-968.pdf
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