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	Provedor de dados BJMBR (2) Autor Alespeiti,G. (2) Hendrikx,J. (2) Nardi,N. (2) Pranke,P. (2) Rubinstein,P. (2) Visser,J. (2) Debnath,G. (1) Mais... Palavra-chave Human umbilical cord blood (2) Adhesion molecules (1) CD34+ (1) CD34+CD38- cells (1) Ex vivo expansion (1) Hematopoietic stem cell (1) Hematopoietic stem cells (1) ISHAGE (1) ProCount (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (1) 2005 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Immunophenotype of hematopoietic stem cells from placental/umbilical cord blood after culture BJMBR Pranke,P.; Hendrikx,J.; Debnath,G.; Alespeiti,G.; Rubinstein,P.; Nardi,N.; Visser,J.. Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin (TPO), FLT-3 ligand (FL) and kit ligand (KL; or stem cell factor) in different combinations: TPO + FL + KL, TPO + FL and TPO, at concentrations of 50 ng/mL each. Cells were immunophenotyped by four-color fluorescence using antibodies against CD11c, CD31, CD49e, CD61, CD62L, CD117, and HLA-DR. Low-density cord blood contained 1.4 ± 0.9% CD34+ cells, 2.6 ± 2.1% of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoietic stem cells; CD34+CD38- cells; Human umbilical cord blood; Ex vivo expansion; Adhesion molecules. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200006 Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the ProCount™-BD and ISHAGE protocols BJMBR Pranke,P.; Hendrikx,J.; Alespeiti,G.; Nardi,N.; Rubinstein,P.; Visser,J.. The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/µL and with the ProCount™ method... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoietic stem cell; Human umbilical cord blood; CD34+; ISHAGE; ProCount. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700008 Registros recuperados: 2 Primeira ... 1 ... Última

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