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	Provedor de dados BJM (1) Autor Ali,Syed Shahid (1) Arshad,Rubina (1) Farooq,Shafqat (1) Palavra-chave Escherichia coli (1) Mutation (1) Penicillin G acylase expression (1) UV irradiation (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Improvement of penicillin G acylase expression in Escherichia coli through UV induced mutations BJM Arshad,Rubina; Farooq,Shafqat; Ali,Syed Shahid. We used ultraviolet (UV) radiation to induce mutation in three locally isolated strains of Escherichia coli. Different dilutions of bacterial cultures were exposed to UV lamp of 254 nm wavelength for different time intervals at varied distances ranging from 5 to 210 sec and 5 to 100 cm. Viable colonies were screened for mutants with an increased production of penicillin G acylase (PGA) and a reduced production of β-lactamase, which are the desired properties of PGA producing industrial strains. A survival curve was made to get optimum exposure time and distance. The survival percentage for each exposure period was calculated and 1-5% survival was found useful for obtaining mutants with desired change. Screening for PGA and β-lactamase constitutive and/or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Mutation; Penicillin G acylase expression; UV irradiation. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400035 Registros recuperados: 1 Primeira ... 1 ... Última

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