Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJMBR (2) Autor Almeida,A.M.P. (2) Carvalho Jr.,L.B. (2) Araujo,A.M. (1) Azevedo,W.M. (1) Barbosa,G.H.T.S. (1) Fatibello-Filho,O. (1) Lima Barros,A.E. (1) Santana,E.M. (1) Mais... Palavra-chave ELISA (2) Polyvinyl alcohol (2) Dot-ELISA (1) Filter paper (1) Glutaraldehyde (1) Immunodetection (1) Plague (1) Polysiloxane (1) Solid phase (1) Yersinia pestis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2002 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA BJMBR Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.. F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this... Tipo: Info:eu-repo/semantics/article Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013 Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA BJMBR Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M.. We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen... Tipo: Info:eu-repo/semantics/other Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco