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| Crystian,Dennis; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero. |
| ABSTRACT: The aim of this study was to analyze the expression of putative reference genes in sugarcane under drought stress. The varieties RB72454 and RB72910 were cultivated and the treatments control and drought stress applied to 135-day-old plants grown under field conditions. The stress level of the plants was measured by rate of photosynthesis, transpiration, and stomatal conductance. For each biological replicate, expression analyses were conducted using quantitative polymerase chain reaction for the genes α-tubulin, β-tubulin, β-actin, cyclophilin, eukaryotic elongation factor 1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), histone H3 and ubiquitin. Stability was evaluated using the geNorm, NormFinder, and BestKeeper software packages. Among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Drought stress; Reference gen; Saccharum. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100203 |
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| Andrade,Júlio César Farias de; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero. |
| Abstract Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (gs) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Plant breeding; Quantitative RT-PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400465 |
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| Santos,Vanessa; Almeida,Cícero. |
| Abstract This study reports the complete chloroplast sequences of three Spondias species. The genome sequences were obtained for Spondias tuberosa, Spondias bahienses, and Spondias mombin using the Illumina sequencing technology by a combination of de novo methods and a reference-guided assembly using Sapindus mukorossi as reference. The genomes of S. tuberosa, S. bahiensis, and S. mombin had 162,036, 162,218, and 162,302 bp, respectively. The coding regions exhibited 130 genes, including 34–35 tRNAs and 4 rRNAs. The results revealed synteny among the genomes, with high conservation in the gene order and content and CG content. The inverted repeat regions (IRA and IRB) and the large and small single copies were very similar among the three genomes. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plastome; Evolution; Spondias. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100132 |
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| Souza,Marislane Carvalho Paz de; Silva,Jéssica Naiana; Almeida,Cícero. |
| Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analyze the repetition of DNA sequences in S. officinarum and S. spontaneum. For this purpose, genomic DNA from S. officinarum was digested with restriction enzymes and the fragments cloned. Sixty-eight fragments, approximately 500 bp, were cloned, sequenced and had their identity analyzed in NCBI, and in the rice, maize, and sorghum genome databases using BLAST. Twelve clones containing partial transposable... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugarcane; Dot-blot hybridization; LTR retrotransposons; Repetitive DNA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300016 |
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| Nobre,Lydayanne Lilás de Melo; Santos,José Daniel Oliveira dos; Leite,Rychard; Almeida,Cícero. |
| Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nucleotide polymorphism (SNP) analysis, characterization of intragenomic nuclear ribosomal DNA (nrDNA), and nuclear and chloroplast phylogenomic analyses. The SNP analysis revealed a high number of SNPs in the S. bahiensis genome, and with respect to nrDNA, S. bahiensis shared approximately half of the SNP alleles with S. tuberosa, but not with S. mombin. Combining the SNP analysis with nrDNA phylogeny... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RDNA; Phylogenetics; Evolution; Hybrid offspring. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500878 |
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| Almeida,Cícero; Amaral,Adriane leite do; Barbosa Neto,José fernandes; Sereno,Maria Jane Cruz de Melo. |
| O armazenamento de pólen é uma importante ferramenta para programas de melhoramento de milho, permitindo preservar, sob condições artificiais, a viabilidade de gametas masculinos e ampliar as possibilidades de cruzamentos, independente da época do florescimento das variedades genitoras. O presente trabalho teve como objetivo avaliar meios de cultivo para a germinação in vitro de pólen de milho e analisar condições de conservação. Para avaliar viabilidade, foram testados seis meios de cultivo, contendo sacarose, ácido bórico, cloreto de cálcio hidratado e ágar. Para a conservação de pólen foram analisadas duas temperaturas (4 ºC e -20 ºC) e dois agentes desidratantes de pólen (sílica gel e cloreto de cálcio hidratado). Os altos valores de viabilidade do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cruzamentos; Estocagem de pólen; Melhoramento genético. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000400003 |
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| Maia,André V.P.; Almeida,Cícero; Santoro,Kleber R.; Melo,João L.A.; Oliveira,José V.; Guedes,Raul N.C.; Badji,César A.. |
| ABSTRACT Neoleucinodes elegantalis is an important tomato pest in Brazil, occurring throughout the country and resulting in economic losses in agriculture. In several species, biogeographic studies in Brazil indicate the structuring of populations, following the refuge model, with a split between the populations of the northeast and the southeast regions of Brazil. The objective of this work was to analyze the phylogeography of N. elegantalis in Brazil, understanding its population structure and the demographic patterns. Larvae were collected from eight locations throughout Brazil, and the mitochondrial cytochrome c oxidase subunit 1 gene was analyzed. A total of 628 bp in 51 individuals were obtained, showing 12 haplotypes with a haplotype diversity of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insect pest; Phylogeography; Small tomato borer. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000300206 |
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