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	Provedor de dados Genet. Mol. Biol. (1) Autor Almeida,Daniela Volcan (1) Figueiredo,Márcio de Azevedo (1) Lanes,Carlos Frederico Ceccon (1) Marins,Luis Fernando (1) Palavra-chave Genetically modified fish (1) Growth hormone cDNA (1) Microinjection (1) Transgenesis (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2007 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy Genet. Mol. Biol. Figueiredo,Márcio de Azevedo; Lanes,Carlos Frederico Ceccon; Almeida,Daniela Volcan; Marins,Luis Fernando. In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenesis; Genetically modified fish; Microinjection; Growth hormone cDNA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008 Registros recuperados: 1 Primeira ... 1 ... Última

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