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Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples BJID
Marques,Fabiana Rangel; Cardoso,Luciana Ventura; Cavasini,Carlos Eugênio; Almeida,Magali Carmem de; Bassi,Nair Aparecida; Almeida,Margarete Teresa Gottardo de; Rossit,Andréa Regina Baptista; Machado,Ricardo Luiz Dantas.
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Parasites; Acquired immunodeficiency syndrome; Cryptosporidium diagnosis; Brazil.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100002
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Efficacy of amphotericin B in a fat emulsion for the treatment of cryptococcal meningitis in AIDS patients BJID
Rubio,Fernando Góngora; Zanon,Jeferson Rodrigo; Almeida,Margarete Teresa Gottardo de; Góngora,Delzi Vinha Nunes de.
Several formulae have been developed in an attempt to reduce the toxicity of amphotericin B (AmB), but their high costs preclude widespread use. The aim of this study was to evaluate the efficacy of amphotericin B in a fat emulsion, i.e. Intralipid (AmB-IL), in 37 AIDS patients with cryptococcal meningitis (CM). We retrospectively reviewed data collected in a non-comparative open study between January 1999 and December 2001. The therapeutic cure was defined as complete resolution or improvement of the clinical symptoms or complete absence or improvement of the mycological alterations of the CSF. The outcomes were evaluated at 2 weeks, induction phase (IP), and at the end of treatment or consolidation phase (CP) with the last available CSF. Prior to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphotericin B; Fat emulsion; Cryptococcal meningitis; AIDS patients.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200006
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