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	Provedor de dados Ciência Rural (2) BJID (1) Autor Almeida,Renata Servan de (3) Arns,Clarice Weis (3) Spilki,Fernando Rosado (2) Baracat,Emílio Carlos Elias (1) Ferreira,Helena Lage (1) Ribeiro,José Dirceu (1) Riccetto,Adriana Gut Lopes (1) Santos,Márcia Mercês Aparecida Bianchi dos (1) Silva,Marcos Tadeu Nolasco da (1) Mais... Palavra-chave Acute respiratory infection (1) Adjuvants (1) Avian metapneumovirus G (1) BRSV (1) Bovine respiratory syncytial virus (1) F (1) Inactivated vaccines (1) Murine model (1) N genes real time RT-PCR RT-PCR (1) Respiratory syncytial virus (1) Risk factors (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2010 (1) 2009 (1) 2006 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Respiratory syncytial virus (RSV) in infants hospitalized for acute lower respiratory tract disease: incidence and associated risks BJID Riccetto,Adriana Gut Lopes; Ribeiro,José Dirceu; Silva,Marcos Tadeu Nolasco da; Almeida,Renata Servan de; Arns,Clarice Weis; Baracat,Emílio Carlos Elias. Respiratory syncytial virus (RSV) is one of the main causes of acute lower respiratory tract infections worldwide. We examined the incidence and associated risks for RSV infection in infants hospitalized in two university hospitals in the state of São Paulo. We made a prospective cohort study involving 152 infants hospitalized for acute lower respiratory tract infections (ALRTI) in two university hospitals in Campinas, São Paulo, Brazil, between April and September 2004. Clinical and epidemiological data were obtained at admission. RSV was detected by direct immunofluorescence of nasopharyngeal secretions. Factors associated with RSV infection were assessed by calculating the relative risk (RR). The incidence of RSV infection was 17.5%. Risk factors... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute respiratory infection; Respiratory syncytial virus; Risk factors. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500011 Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants Ciência Rural Spilki,Fernando Rosado; Almeida,Renata Servan de; Arns,Clarice Weis. Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine respiratory syncytial virus; BRSV; Adjuvants; Murine model; Inactivated vaccines. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013 Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A Ciência Rural Ferreira,Helena Lage; Spilki,Fernando Rosado; Santos,Márcia Mercês Aparecida Bianchi dos; Almeida,Renata Servan de; Arns,Clarice Weis. Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F- based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10¹ TCID50 mL-1). The present study suggests that the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian metapneumovirus G; F; N genes real time RT-PCR RT-PCR. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500022 Registros recuperados: 3 Primeira ... 1 ... Última

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