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Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil BABT
Minozzo,João Carlos; Moura,Juliana de; Almeida,Sérgio Monteiro; Thomaz-Soccol,Vanete.
Neurocysticercosis (NCC), the cerebral presence of Taenia solium metacestode (Cysticercus cellulosae), is responsible for neurological disorders worldwide. In order to validate an immunodiagnosis for public-health patients in the State of Parana-Brazil, crude antigen of Taenia crassicepsmetacestode (Cysticercus longicollis) was used as an alternative heterologous antigen to be used in ELISA and in electroimmunotransfer blotting (EITB) for active and inactive NCC diagnosis. Indirect ELISA was able to discriminate between active and inactive samples and presented high specificity and sensitivity. Any immunodominant band was able to distinguish the NCC stages, although the EITB showed 100% specificity. The immunological results proved to be an important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurocysticercosis; Immunodiagnosis; ELISA; EITB; Cysticercus longicollis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600007
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Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients BJID
Ferreira,Carla Elizabeth de Oliveira; Raboni,Sonia Mara; Pereira,Luciane Aparecida; Nogueira,Meri Bordignon; Vidal,Luine Rosele Renaud; Almeida,Sérgio Monteiro.
BACKGROUND: Acute gastroenteritis (AGE) is a common disorder that affects children worldwide. It is usually caused by viral agents, including rotavirus, enteric adenovirus, norovirus, and astrovirus groups. Currently, there are few reports about co-infection among these viruses, mainly in Brazil. METHODS: This is a retrospective study in which 84 rotavirus-positive samples from hospitalized patients at a teaching hospital in Southern Brazil, collected in the 2001-2010 period, were analyzed by polymerase chain reaction (PCR) and reverse transcription - polymerase chain reaction (RT-PCR), for the investigation of enteric adenovirus, astrovirus, and norovirus. RESULTS: In total, 12 of the 84 (14%) samples were positive to enteric adenovirus or norovirus....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-infection; Viral gastroenteritis; Oiarrhea; Rotavirus A; Norovirus; Enteric adenovirus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300009
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