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| Santana Filho,F.C.; Silva,J.A.; Magalhães,D.F.; Meneses,J.N.C.; Haddad,J.P.A.; Morais,M.H.F.; Almeida,V.G.. |
| Os objetivos desta pesquisa foram caracterizar as recusas por parte da população em permitir a borrifação de seus imóveis, em quatro regiões de Belo Horizonte, no período de 2006 a 2008, e relacionar estas recusas com o surgimento de casos de leishmaniose visceral, no mesmo local e período. Utilizaram-se as localizações onde se encontraram 27 casos humanos de leishmaniose visceral obtidos do SINAN, todos referentes a quatro áreas de abrangência da região noroeste de Belo Horizonte, no período de 2006 a 2008. De um total de 33.579 residências visitadas pelo controle químico vetorial, 9636 (28,70%) aceitaram a borrifação interna e a externa do domicílio, 20741 (61,77%) concordaram com a borrifação apenas do peridomicílio, 141 (0,42%) aceitaram apenas a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Recusa de participação; Controle de vetores; Leishmaniose visceral. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400017 |
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| Almeida,V.G.; Avelar-Freitas,B.A.; Santos,M.G.; Costa,L.A.; Silva,T.J.; Pereira,W.F.; Amorim,M.L.L.; Grael,C.F.F.; Gregório,L.E.; Rocha-Vieira,E.; Brito-Melo,G.E.A.. |
| Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokines; Lymphocyte proliferation; Luteolin; Medicinal plant; Pseudobrickellia brasiliensis; Quinic acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800603 |
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| Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.. |
| Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307 |
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