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	Provedor de dados BJMBR (2) Autor Amaral,L.M. (2) Costa,M.L. (2) Mermelstein,C.S. (2) Rebello,M.I.L. (2) Borojevic,R. (1) Reis,J.S.N. (1) Mais... Palavra-chave Extracellular Ca2+ (2) Cell shape (1) Cytoskeleton (1) EGTA Myoblasts Desmin Intermediate filaments RT-PCR (1) Integrin (1) Microfilaments (1) Microtubules (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation BJMBR Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.. Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018 Changes in cell shape and desmin intermediate filament distribution are associated with down-regulation of desmin expression in C2C12 myoblasts grown in the absence of extracellular Ca2+ BJMBR Mermelstein,C.S.; Amaral,L.M.; Rebello,M.I.L.; Reis,J.S.N.; Borojevic,R.; Costa,M.L.. Desmin is the main intermediate filament (IF) protein of muscle cells. In skeletal muscle, desmin IFs form a scaffold that interconnects the entire contractile apparatus with the subsarcolemmal cytoskeleton and cytoplasmic organelles. The interaction between desmin and the sarcolemma is mediated by a number of membrane proteins, many of which are Ca2+-sensitive. In the present study, we analyzed the effects of the Ca2+ chelator EGTA (1.75 mM) on the expression and distribution of desmin in C2C12 myoblasts grown in culture. We used indirect immunofluorescence microscopy and reverse transcription polymerase chain reaction (RT-PCR) to analyze desmin distribution and expression in C2C12 cells grown in the presence or absence of EGTA. Control C2C12 myoblasts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular Ca2+; EGTA Myoblasts Desmin Intermediate filaments RT-PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700005 Registros recuperados: 2 Primeira ... 1 ... Última

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