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| Jensen, A.N.; Andersen, M.T.; Dalsgaard, A.; Baggesen, D.L.; Nielsen, E.M.. |
| Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for species-specific identification of C. jejuni, C. coli, C. lari, C. upsaliensis and of a hipO gene nucleotide probe for detection of C. jejuni by colony-blot hybridization were determined by testing a total of 75 reference strains of Campylobacter spp. and related organisms. The rt-PCR method allowed species-specific detection of Campylobacter spp. in naturally infected pig faecal samples after an enrichment step, whereas the hybridization approach enhanced the specific isolation of C. jejuni (present in... |
| Tipo: Journal paper |
Palavras-chave: Pigs. |
| Ano: 2005 |
URL: http://orgprints.org/4804/1/4804.pdf |
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