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Biological evaluation of recombinant human erythropoietin in pharmaceutical products BJMBR
Ramos,A.S.; Schmidt,C.A.; Andrade,S.S.; Fronza,M.; Rafferty,B.; Dalmora,S.L..
The potencies of mammalian cell-derived recombinant human erythropoietin pharmaceutical preparations, from a total of five manufacturers, were assessed by in vivo bioassay using standardized protocols. Eight-week-old normocythemic mice received a single subcutaneous injection followed by blood sampling 96 h later or multiple daily injections with blood sampling 24 h after the last injection. Reticulocyte counting by microscopic examination was employed as the end-point using the brilliant cresyl blue or selective hemolysis methods, together with automated flow cytometry. Different injection schedules were investigated and dose-response curves for the European Pharmacopoeia Biological Reference Preparation of erythropoietin were compared. Manual and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recombinant human erythropoietin; Bioassay; Polycythemia; Reticulocyte counting; Normocythemic mice.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100014
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New multilocus sequence typing of MRSA in São Paulo, Brazil BJMBR
Carmo,M.S.; Inoue,F.; Andrade,S.S.; Paschoal,L.; Silva,F.M.; Oliveira,V.G.S.; Pignatari,A.C.C..
An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular sequence typing; Resistance; Molecular typing.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000006
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