|
|
|
|
|
| Angelo,Ana Luiza Dias; Angelo,Camila Dias; Torres,Alex José Leite; Ramos,André Maurício Costa; Lima,Márcia; Netto,Eduardo Martins; Brites,Carlos. |
| This study evaluated total lymphocyte count (TLC) as a substitute marker for CD4+ cell counts to identify patients who need prophylaxis against opportunistic infection (CD4 < 200 cells/mm³) and patients with CD4 < 350 cells/mm³ (Brazilian threshold value of CD4 count to define AIDS). We evaluated TLC and CD4+ cells count of 1,174 HIV-infected patients, in Salvador, Brazil, from May 2003 to September 2004. CD4+ cell counts were performed by flow cytometry, and TLC was measured with an automated hematological counter. The mean CD4 count was 430 cells/mm³ (range: 4 to 2,531 cells/mm³). Mean TLC was 1,900 cells/mm³ (range: 300 to 6,200 cells/mm³). Using a threshold value of 1,000 cells/mm³ for TLC, the positive predictive value (PPV) was 77% for CD4 <... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lymphocyte counts; CD4 counts; AIDS. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500005 |
| |
|
|
| Torres,Alex José Leite; Brígido,Luis Fernando de Macedo; Abrahão,Marcos Herculano Nunes; Angelo,Ana Luiza Dias; Ferreira,Gilcivaldo de Jesus; Coelho,Luana Portes; Ferreira,João Leandro; Jorge,Célia Regina Mayoral Pedroso; Martins Netto,Eduardo; Brites,Carlos. |
| Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a flow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identified by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identification of X4 and R5 tropic viruses was attempted by geno2pheno... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Tropism; Flow cytometry; Geno2pheno. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200163 |
| |
|
|
|
