| Seven wheat cultivars having some degree of resistance to Bipolaris sorokiniana, Magnaporthe grisea or Xanthomonas campestris pv. undulosa (Xcu) provided somaclonal variation for disease resistance. Callus induction varied from 69.4 to 100% across the cultivars, whereas regeneration frequency of R1 plants (regenerated plants of first generation) varied between 2.7 and 23.1%. Resistance variation in the R2-regenerated second generation plants was observed for B. sorokiniana and M. grisea but not for Xcu. Attempts were made to fix the resistance characteristics of R3-regenerated third generation somaclones through wheat x maize hybrids. Wheat and maize hybridization of seven wheat somaclones yielded 81 embryos. A total of 11,624 somaclone florets were... |
