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| Lima,Dirlene P.; Azevedo,Jorge F. de; Hermes-Uliana,Catchia; Alves,Gilberto; Sant'ana,Débora M.G.; Araújo,Eduardo J.A.. |
| The objective of this study was to analyze morphometrically the colon wall strata of malnourished rats supplemented with probiotics. Sixteen recently weaned Wistar rats (Rattus norvegicus) were distributed into four groups: animals that received commercial chow (G1, n = 4); animals that received the same feed as G1 and were supplemented with probiotics (G2, n = 4); animals that received chow with 4% of proteins (G3, n = 4); animals that received the same feed as G3 and were supplemented with probiotics (G4, n = 4). After 12 weeks, the proximal colon was collected and submitted to histological processing. Three-µm cuts were stained with H.E., Periodic Acid Schifff (P.A.S.) + diasthasis solution and Alcian Blue (A.B.) pH 2.5 and pH 1.0. The morphometric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Histology; Large intestine; Malnourishment; Morphometry; Probiotics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300015 |
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| Papazian-Cabanas,Rodrigo M.; Araújo,Eduardo J.A.; Silva,Aristeu V. da; Sant'Ana,Débora M.G.. |
| The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4); Acute Experimental Group (AEG, n=4); Chronic Control Group (CCG, n=5); and Chronic Experimental Group (CEG, n=5). Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain) tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Infection; Parasite; Toxoplasmosis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300016 |
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| Sant'Ana,Débora M.G.; Araújo,Eduardo J.A.; Ramos,Diego H.; Hermes-Uliana,Catchia; Natali,Maria Raquel M.. |
| The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Malnutrition; Myenteric plexus; Nitrergic neurons. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300022 |
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